中国基层医药
中國基層醫藥
중국기층의약
CHINESE JOURNAL OF PRIMARY MEDICINE AND PHARMACY
2013年
5期
647-649
,共3页
宫颈肿瘤%PTEN%HR-HPV%免疫组织化学
宮頸腫瘤%PTEN%HR-HPV%免疫組織化學
궁경종류%PTEN%HR-HPV%면역조직화학
Cervical neoplasm%PTEN%High risk-human papillomavirus%Immunohistochemistry
目的 通过检测高危型HPV(HR-HPV)和抑癌基因PTEN在宫颈上皮内瘤样病变(CIN)和宫颈微小浸润鳞癌(MICA)中的表达,并对比治疗一年后表达变化,探讨HR-HPV感染和PTEN在宫颈上皮癌变过程中的作用及其相互关系.方法 应用免疫组织化学(SP)法检测PTEN蛋白在30例正常宫颈组织、37例宫颈轻度上皮内瘤样病变Ⅰ级(CIN Ⅰ)、50例宫颈中度上皮内瘤样病变Ⅱ级(CINⅡ)、50例宫颈重度上皮内瘤样病变Ⅲ级(CINⅢ)和25例宫颈微小型浸润鳞癌(MICA)中的表达.应用第二代杂交捕获法(HC-Ⅱ)检测每一病例的13种HR-HPV DNA.CIN Ⅰ、Ⅱ、Ⅲ组病例均在一年后随访再次检测PTEN蛋白和HR-HPVDNA.结果 HR-HPV DNA在CIN Ⅰ (15/37,40.50％),CINⅡ(28/50,56.00％),CINⅢ(32/50,64.00％)及MICA(24/25,96.00％)中表达均高于正常组织(1/30,3.33％),差异有统计学意义(P＜0.01).HR-HPV在CIN Ⅰ、Ⅱ、Ⅲ及MICA中的表达,病变级别越高,表达率越高,差异有统计学意义(P＜0.01).PTEN表达在正常宫颈组100％中、强阳性表达(30/30);阳性表达率各组分别为:CIN Ⅰ组100％,CINⅡ组48％,CINⅢ组40％,MICA组16％.PTEN蛋白在正常宫颈、CIN和MICA各组组织中阳性表达率逐渐降低.其中MICA组与其余各组差异均有统计学意义(P＜0.01)；CIN Ⅰ组与正常宫颈组差异无统计学意义(P ＞0.05)；CINⅡ组与CINⅢ组差异无统计学意义(P ＞0.05)；CIN Ⅲ组、CINⅡ组与CIN Ⅰ组及正常宫颈组比较,差异有统计学意义(P＜0.05).结论 HR-HPV感染的宫颈病变中,PTEN呈阴性或低表达,PTEN可能与HR-HPV DNA持续作用有关,其功能下调促进了宫颈的癌变进程.
目的 通過檢測高危型HPV(HR-HPV)和抑癌基因PTEN在宮頸上皮內瘤樣病變(CIN)和宮頸微小浸潤鱗癌(MICA)中的錶達,併對比治療一年後錶達變化,探討HR-HPV感染和PTEN在宮頸上皮癌變過程中的作用及其相互關繫.方法 應用免疫組織化學(SP)法檢測PTEN蛋白在30例正常宮頸組織、37例宮頸輕度上皮內瘤樣病變Ⅰ級(CIN Ⅰ)、50例宮頸中度上皮內瘤樣病變Ⅱ級(CINⅡ)、50例宮頸重度上皮內瘤樣病變Ⅲ級(CINⅢ)和25例宮頸微小型浸潤鱗癌(MICA)中的錶達.應用第二代雜交捕穫法(HC-Ⅱ)檢測每一病例的13種HR-HPV DNA.CIN Ⅰ、Ⅱ、Ⅲ組病例均在一年後隨訪再次檢測PTEN蛋白和HR-HPVDNA.結果 HR-HPV DNA在CIN Ⅰ (15/37,40.50％),CINⅡ(28/50,56.00％),CINⅢ(32/50,64.00％)及MICA(24/25,96.00％)中錶達均高于正常組織(1/30,3.33％),差異有統計學意義(P＜0.01).HR-HPV在CIN Ⅰ、Ⅱ、Ⅲ及MICA中的錶達,病變級彆越高,錶達率越高,差異有統計學意義(P＜0.01).PTEN錶達在正常宮頸組100％中、彊暘性錶達(30/30);暘性錶達率各組分彆為:CIN Ⅰ組100％,CINⅡ組48％,CINⅢ組40％,MICA組16％.PTEN蛋白在正常宮頸、CIN和MICA各組組織中暘性錶達率逐漸降低.其中MICA組與其餘各組差異均有統計學意義(P＜0.01)；CIN Ⅰ組與正常宮頸組差異無統計學意義(P ＞0.05)；CINⅡ組與CINⅢ組差異無統計學意義(P ＞0.05)；CIN Ⅲ組、CINⅡ組與CIN Ⅰ組及正常宮頸組比較,差異有統計學意義(P＜0.05).結論 HR-HPV感染的宮頸病變中,PTEN呈陰性或低錶達,PTEN可能與HR-HPV DNA持續作用有關,其功能下調促進瞭宮頸的癌變進程.
목적 통과검측고위형HPV(HR-HPV)화억암기인PTEN재궁경상피내류양병변(CIN)화궁경미소침윤린암(MICA)중적표체,병대비치료일년후표체변화,탐토HR-HPV감염화PTEN재궁경상피암변과정중적작용급기상호관계.방법 응용면역조직화학(SP)법검측PTEN단백재30례정상궁경조직、37례궁경경도상피내류양병변Ⅰ급(CIN Ⅰ)、50례궁경중도상피내류양병변Ⅱ급(CINⅡ)、50례궁경중도상피내류양병변Ⅲ급(CINⅢ)화25례궁경미소형침윤린암(MICA)중적표체.응용제이대잡교포획법(HC-Ⅱ)검측매일병례적13충HR-HPV DNA.CIN Ⅰ、Ⅱ、Ⅲ조병례균재일년후수방재차검측PTEN단백화HR-HPVDNA.결과 HR-HPV DNA재CIN Ⅰ (15/37,40.50％),CINⅡ(28/50,56.00％),CINⅢ(32/50,64.00％)급MICA(24/25,96.00％)중표체균고우정상조직(1/30,3.33％),차이유통계학의의(P＜0.01).HR-HPV재CIN Ⅰ、Ⅱ、Ⅲ급MICA중적표체,병변급별월고,표체솔월고,차이유통계학의의(P＜0.01).PTEN표체재정상궁경조100％중、강양성표체(30/30);양성표체솔각조분별위:CIN Ⅰ조100％,CINⅡ조48％,CINⅢ조40％,MICA조16％.PTEN단백재정상궁경、CIN화MICA각조조직중양성표체솔축점강저.기중MICA조여기여각조차이균유통계학의의(P＜0.01)；CIN Ⅰ조여정상궁경조차이무통계학의의(P ＞0.05)；CINⅡ조여CINⅢ조차이무통계학의의(P ＞0.05)；CIN Ⅲ조、CINⅡ조여CIN Ⅰ조급정상궁경조비교,차이유통계학의의(P＜0.05).결론 HR-HPV감염적궁경병변중,PTEN정음성혹저표체,PTEN가능여HR-HPV DNA지속작용유관,기공능하조촉진료궁경적암변진정.
Objective To detect the correlations of HR-HPV infection with expression of cancer suppressor gene-PTEN in CIN and MICA compared to a year after treatment,to investigate clinical significance in the carcinogenesis of cervical epithelium.Methods SP method was applied to detect the expression of PTEN in normal cervical tissues (30 cases),cervical intraepithelial neoplasia CIN Ⅰ 47cases,CIN Ⅱ 50 cases,CIN Ⅲ 50 cases and early cervical squamous cell carcinoma (25cases).13 types of HR-HPV DNA in these cases were detected by Hybrid Capture 2 (HC-Ⅱ) assay.The expression of PTEN and HR-HPV were all detected in cervical intraepithelial neoplasia (CIN Ⅰ,CIN Ⅱ,CIN Ⅲ) after one year.Results The positive rates of HR-HPV in CIN Ⅰ (31.91％),CIN Ⅱ (56.00％),CINⅢ (64.00％) and MICA (96.00％) were higher than those in normal tissues (96.00％,P ＜0.01).Cervical intraepithelial neoplasia with higher grade showed higher expression of HR-HPV (P ＜ 0.01),while the lower expression of PTEN,as moderate or strong expression in normal tissue,100％ in CIN Ⅰ,48％ in CIN Ⅱ,40％ in CIN Ⅲ,16％ in MICA.There was significance between MICA and other groups (P ＜ 0.01),so it was cervical intraepithelial neoplasia (Ⅰ,Ⅱ,Ⅲ) and normal tissue (P ＜ 0.01).Condusion In cervical changes with continous infection of HR-HPV,negative or low expressions of PTEN were found,PTEN might be concerned with the continous action of HR-HPV,which might promote cevical carcinogenesis due to its decreased function.
