中国基层医药
中國基層醫藥
중국기층의약
CHINESE JOURNAL OF PRIMARY MEDICINE AND PHARMACY
2013年
20期
3044-3046
,共3页
盛建华%吴建%龚燕萍%沈艳红%蒋廷旺
盛建華%吳建%龔燕萍%瀋豔紅%蔣廷旺
성건화%오건%공연평%침염홍%장정왕
脊柱炎,强直性%白细胞介素27%CD4淋巴细胞计数
脊柱炎,彊直性%白細胞介素27%CD4淋巴細胞計數
척주염,강직성%백세포개소27%CD4림파세포계수
Spondylitis,ankylosing%Interleukin-27%CD4 lymphocyte count
目的 探讨白细胞介素-27(IL-27)在强直性脊柱炎(AS)患者外周血的表达及其对CD4+T细胞的增殖分化作用机制.方法 分别收集AS患者、健康体检者(Health controls,HCs)的外周血,磁珠分离CD4+T细胞.ELISA测定IL-27及其相关细胞因子的表达.IL-27体外作用后,CCK-8测定细胞增殖情况,定量PCR分析T-bet和GATA3基因表达情况.结果 AS患者外周血IL-27显著高于HCs组(P＜0.01),IL-27作用后,AS组和HCs组CD4+T细胞增殖能力均增强,AS组CD4+T细胞增殖能力强于HCs组(t=12.1,P＜0.01),同时AS组细胞培养液中IFN-γ在IL-27作用后较HCs组均显著增高(P＜0.01),IL-2及IL-10表达无明显变化.IL-27诱导后AS组CD4+T淋巴细胞T-bet基因表达显著增加(t=14.3,P＜0.01),而GATA3差异无统计学意义.结论 IL-27在AS患者外周血表达升高,并可诱导CD4+T细胞增殖,可能通过上调T-bet的表达诱导CD4+T细胞向Th1分化,可能在AS免疫炎性反应过程中发挥重要作用.
目的 探討白細胞介素-27(IL-27)在彊直性脊柱炎(AS)患者外週血的錶達及其對CD4+T細胞的增殖分化作用機製.方法 分彆收集AS患者、健康體檢者(Health controls,HCs)的外週血,磁珠分離CD4+T細胞.ELISA測定IL-27及其相關細胞因子的錶達.IL-27體外作用後,CCK-8測定細胞增殖情況,定量PCR分析T-bet和GATA3基因錶達情況.結果 AS患者外週血IL-27顯著高于HCs組(P＜0.01),IL-27作用後,AS組和HCs組CD4+T細胞增殖能力均增彊,AS組CD4+T細胞增殖能力彊于HCs組(t=12.1,P＜0.01),同時AS組細胞培養液中IFN-γ在IL-27作用後較HCs組均顯著增高(P＜0.01),IL-2及IL-10錶達無明顯變化.IL-27誘導後AS組CD4+T淋巴細胞T-bet基因錶達顯著增加(t=14.3,P＜0.01),而GATA3差異無統計學意義.結論 IL-27在AS患者外週血錶達升高,併可誘導CD4+T細胞增殖,可能通過上調T-bet的錶達誘導CD4+T細胞嚮Th1分化,可能在AS免疫炎性反應過程中髮揮重要作用.
목적 탐토백세포개소-27(IL-27)재강직성척주염(AS)환자외주혈적표체급기대CD4+T세포적증식분화작용궤제.방법 분별수집AS환자、건강체검자(Health controls,HCs)적외주혈,자주분리CD4+T세포.ELISA측정IL-27급기상관세포인자적표체.IL-27체외작용후,CCK-8측정세포증식정황,정량PCR분석T-bet화GATA3기인표체정황.결과 AS환자외주혈IL-27현저고우HCs조(P＜0.01),IL-27작용후,AS조화HCs조CD4+T세포증식능력균증강,AS조CD4+T세포증식능력강우HCs조(t=12.1,P＜0.01),동시AS조세포배양액중IFN-γ재IL-27작용후교HCs조균현저증고(P＜0.01),IL-2급IL-10표체무명현변화.IL-27유도후AS조CD4+T림파세포T-bet기인표체현저증가(t=14.3,P＜0.01),이GATA3차이무통계학의의.결론 IL-27재AS환자외주혈표체승고,병가유도CD4+T세포증식,가능통과상조T-bet적표체유도CD4+T세포향Th1분화,가능재AS면역염성반응과정중발휘중요작용.
Objective To investigate the role of interleukin-27 (IL-27) in proliferation and differentiation of CD4+ T cells in ankylosing spondylitis(AS).Methods CD4+ T cells were separated from peripheral blood which collected from AS patients and health controls(HCs).Cells proliferation was detected by CCK-8 kit,and cytokines were analyzed by ELISA.Real-time PCR was used to determine the mRNA expression of T-bet and GATA3.Results Serum IL-27 level in patients with AS was obviously higher than that in HCs(P ＜0.01).The proliferation rate of CD4+ T cells and the level of IFN-γin cultured medium in AS were higher than those in HCs group after IL-27 stimulation (P ＜ 0.01).IL-27 could induce T-bet mRNA expression in CD4+ T cells in AS (t =14.3,P ＜ 0.01),but no change was found in GATA3 mRNA expression.Conclusions IL-27 could induce the proliferation of CD4+ T cells and the activation of T-bet pathway in AS.Furthermore,Th1 immune response and related cytokines could be induced by IL-27 in AS.These implicate that IL-27 may play an important role in AS related inflammation.
