CAJ | 학술논문

目的探讨建立小鼠肝癌原位模型并观察其生物治疗的效果.方法选取Balb/c小鼠来源的ML-3肝癌细胞经肝内直接注射接种至肝脏实质内,与经脾注射肝癌细胞相比较,对其成瘤情况进行观察；并予以生物治疗,观察其疗效.结果肿瘤细胞接种第6天起,经脾脏注射组以及经肝脏注射各组小鼠均不同程度出现体质量减轻、腹水、黄疸以及腹腔可触及包快.在经脾脏注射组小鼠中,5只小鼠中仅有1只小鼠长出肝脏肿块.其余均腹腔种植转移.在经肝脏直接注射小鼠中,15只小鼠中有10只长出肝癌,腹腔中没有种植转移8例.DC肿瘤疫苗联合OX86单抗治疗组小鼠的全身状况要优于疫苗单独治疗组以及PBS对照组.分别选取对照组小鼠以及治疗组小鼠的脾脏细胞以及腹水细胞样本进行流式细胞分析,治疗组脾脏和腹水细胞中MDSCs,即表达CD11b+ LyC6+细胞(2.77％)低于对照组(11.94％)(x2=5.36,P＜0.05).结论经肝脏直接注射肿瘤细胞较经脾脏注射法能更有效诱导肝癌原位模型,且生物治疗对于该肝癌实验模型有一定效果.
목적 탐토건립소서간암원위모형병관찰기생물치료적효과.방법 선취Balb/c소서래원적ML-3간암세포경간내직접주사접충지간장실질내,여경비주사간암세포상비교,대기성류정황진행관찰；병여이생물치료,관찰기료효.결과 종류세포접충제6천기,경비장주사조이급경간장주사각조소서균불동정도출현체질량감경、복수、황달이급복강가촉급포쾌.재경비장주사조소서중,5지소서중부유1지소서장출간장종괴.기여균복강충식전이.재경간장직접주사소서중,15지소서중유10지장출간암,복강중몰유충식전이8례.DC종류역묘연합OX86단항치료조소서적전신상황요우우역묘단독치료조이급PBS대조조.분별선취대조조소서이급치료조소서적비장세포이급복수세포양본진행류식세포분석,치료조비장화복수세포중MDSCs,즉표체CD11b+ LyC6+세포(2.77％)저우대조조(11.94％)(x2=5.36,P＜0.05).결론 경간장직접주사종류세포교경비장주사법능경유효유도간암원위모형,차생물치료대우해간암실험모형유일정효과.
Objective To establish an orthotropic tumor model for hepatocellular carcinoma in mice and to explore the effect of biotherapy on this tumor model.Methods ML-3 hepatocellular carcinoma cells derived from Balb/c mice were injected into the mice through intrahepatic injection and intra-spleen injection.The tumor formation and the survival of the mice were observed and the effect of tumor vaccine was studied preliminarily.Results The weight reduction,ascites,jaundice and abdominal mass were found on the 6th day of tumor cells injection.Liver tumor was oberserved in 1 mouse by introspleen injection,and implantation metastasis was found in the other 4 mice.Among the 15 mice through intrahepatic injection,liver tumor was found in 10 mice and 8 mice without implantation metastasis.The overall outcome of the mice received DC tumor vaccine combined with OX86 antibody was better than the vaccine alone and PBS control group.Spleen cell and ascites cell samples of control and treatment group were selected and analyzed by flow cytometry.MDSCs cell of the treatment group which expressed CD11b+ LyC6+ (2.77％) was lower than that of control group (11.94％) (x2 =5.36,P ＜ 0.05).Conclusion The orthotropic tumor model for hepatocellular carcinoma in mice was successfully established by intrahepatic injection rather than intra-spleen injection,and tumor biotherapy was to some extent effective to this orthotropic model.