CAJ | 학술논문

目的探讨HE染片改做免疫组织化学染色时采用不同退色方法对组织抗原表达的影响.方法利用免疫组织化学过程中的抗原修复液(EDTA或柠檬酸溶液)对HE染片进行抗原热修复退色,并与盐酸溶液常温退色法进行比较.结果抗原热修复退色完全彻底,与其它方法相比,退色时间短(1～2 min),完全退色率均为90％以上,差异均有统计学意义(x2＝27.6、23.9,均P＜0.01),且不损伤染片,免疫组织化学结果准确,无背景着色和异常着色,抗原表达率及强度高.结论抗原热修复退色不但简化了染色程序,而且能较好地保护组织抗原活性,是目前较好的退色及保护抗原的方法,在病理诊断中具有一定的实用价值.
목적 탐토HE염편개주면역조직화학염색시채용불동퇴색방법대조직항원표체적영향.방법 이용면역조직화학과정중적항원수복액(EDTA혹저몽산용액)대HE염편진행항원열수복퇴색,병여염산용액상온퇴색법진행비교.결과 항원열수복퇴색완전철저,여기타방법상비,퇴색시간단(1～2 min),완전퇴색솔균위90％이상,차이균유통계학의의(x2＝27.6、23.9,균P＜0.01),차불손상염편,면역조직화학결과준학,무배경착색화이상착색,항원표체솔급강도고.결론 항원열수복퇴색불단간화료염색정서,이차능교호지보호조직항원활성,시목전교호적퇴색급보호항원적방법,재병리진단중구유일정적실용개치.
Objective To explore the different methods of decoloration for immunohistochemical HE slides,and their influence on tissue antigen expression.Methods Antigen retrieval solution (EDTA or citric acid solution) was used in immunohistochemistry to decolor HE slides,and was compared with the method of hydrochloric acid solution decoloration in normal temperature.Results Antigen heat retrieval decolors completely.Compared with other methods,it has shorter decoloration time (1 ～ 2min),more than 90％ of completely decoloration rate,the difference was significant(x2 =27.6,23.9,all P ＜ 0.01).Meanwhile,the method dose not damage slides,and the immunohistoehemical results are exact,without background coloring and unusual coloring,antigen expression rate and intensity are high.Conclusion Antigen heat retrieval decoloration not only simplifies the coloration process,but also protects tissue antigen activity better.It is the preferable method of decoloration and protecting antigen resource,which possesses some practical value in the pathology diagnosis.