CAJ | 학술논문

目的观察不明原因复发性流产(URSA)患者蜕膜组织中蛋白激酶(SGK)的表达及其在介导细胞凋亡中的相关性.方法采用免疫组织化学SP法检测URSA患者组50例(流产组)和30例人工流产(对照组)蜕膜组织中SGK的表达情况,应用TUNEL法检测两组细胞凋亡指数.结果流产组中SGK的阳性表达率(24.0％)显著低于对照组(93.3％),差异有统计学意义(x2=6.78,P ＜0.05).流产组的平均细胞凋亡指数[(8.19±3.58)％]高于对照组[(2.87±1.07)％],差异有统计学意义(t=7.94,P＜0.05).结论蜕膜组织细胞凋亡明显增加、SGK表达降低可能在URSA中具有重要作用.
목적 관찰불명원인복발성유산(URSA)환자세막조직중단백격매(SGK)적표체급기재개도세포조망중적상관성.방법 채용면역조직화학SP법검측URSA환자조50례(유산조)화30례인공유산(대조조)세막조직중SGK적표체정황,응용TUNEL법검측량조세포조망지수.결과 유산조중SGK적양성표체솔(24.0％)현저저우대조조(93.3％),차이유통계학의의(x2=6.78,P ＜0.05).유산조적평균세포조망지수[(8.19±3.58)％]고우대조조[(2.87±1.07)％],차이유통계학의의(t=7.94,P＜0.05).결론 세막조직세포조망명현증가、SGK표체강저가능재URSA중구유중요작용.
Objective To observe the expression of SGK in decidua in patients with unexplained recurrent spontaneous abortion(URSA) and to investigate its role in the course of apoptosis.Methods The expression of SGK was detected by immunohistochemistry in decidua of URSA(abortion group,n =50) and normal first trimester pregnant women(control group,n =30).The apoptotic index was detected by TUNEL.Results Compared with the control group,the positive expression of SGK decreased significantly in the abortion group,and the difference was statistically significant(x2 =6.78,P ＜ 0.05).The average apoptotic index of the abortion group was (8.19 ± 3.58) ％,which was significantly higher than (2.87 ±1.07)％ of the control group(t =7.94,P＜0.05).Conclusion Intensive apoptosis,the decreasing positive expression rate of SGK in decidua cells may play critical roles in URSA.