CAJ | 학술논문

目的研究胶原海绵对创面愈合的影响及其对创面炎性反应干预机制.方法将24只大鼠按笼号奇偶数随机分为对照组、实验组,每组12只,造模大鼠背部机械性全层皮肤创面,以0.9％氯化钠溶液纱布(对照组)、胶原海绵(实验组)覆盖创面,隔天更换;造模时及造模后第3、7、14、28天测定创面面积,造模后第3、7天测炎症积分,造模时及造模后第3、7天取创面组织检测TNF-d与IL-6含量.结果治疗后7d,实验组创面面积[(0.49 ±0.08) cm2]显著小于对照组[(0.92±0.11)cm2];至28 d时,两组创面完全愈合.造模后3、7d,对照组创面肉芽炎症积分分别为(4.51±1.01)分、(2.25±0.62)分,实验组分别为(3.58±0.51)分、(1.66±0.49)分,两组差异均有统计学意义(t=2.85、2.59,均P＜0.01).对照组造模后7 dTNF-α含量为(512 ±62)ng·L-1·g-1,高于实验组的(434±37)ng·L-1·g-1(=3.74,P ＜0.05);对照组第3天、第7天时肉芽组织中IL-6含量分别为(2 215 ±314)ng·L-1·g-1、(1 562±174)ng·L-1·g-1,实验组分别为(1 672±161)ng·L-1·g-1、(669±75)ng · L-1·g-1,两组差异均有统计学意义(=5.33、16.33,均P＜0.01).结论胶原海绵可以发挥干预创面炎性反应及促进创面血管新生疗效,促进创面愈合.
목적 연구효원해면대창면유합적영향급기대창면염성반응간예궤제.방법 장24지대서안롱호기우수수궤분위대조조、실험조,매조12지,조모대서배부궤계성전층피부창면,이0.9％록화납용액사포(대조조)、효원해면(실험조)복개창면,격천경환;조모시급조모후제3、7、14、28천측정창면면적,조모후제3、7천측염증적분,조모시급조모후제3、7천취창면조직검측TNF-d여IL-6함량.결과 치료후7d,실험조창면면적[(0.49 ±0.08) cm2]현저소우대조조[(0.92±0.11)cm2];지28 d시,량조창면완전유합.조모후3、7d,대조조창면육아염증적분분별위(4.51±1.01)분、(2.25±0.62)분,실험조분별위(3.58±0.51)분、(1.66±0.49)분,량조차이균유통계학의의(t=2.85、2.59,균P＜0.01).대조조조모후7 dTNF-α함량위(512 ±62)ng·L-1·g-1,고우실험조적(434±37)ng·L-1·g-1(=3.74,P ＜0.05);대조조제3천、제7천시육아조직중IL-6함량분별위(2 215 ±314)ng·L-1·g-1、(1 562±174)ng·L-1·g-1,실험조분별위(1 672±161)ng·L-1·g-1、(669±75)ng · L-1·g-1,량조차이균유통계학의의(=5.33、16.33,균P＜0.01).결론 효원해면가이발휘간예창면염성반응급촉진창면혈관신생료효,촉진창면유합.
Objective To explore the effect of collagen sponge on wound healing and its mechanism of regulating the response of wound inflammation.Methods 24 rats were divided into the control group and the experimental group by even and odd numbers of cages,12 rats in each group.Collagen sponges were applied locally on the round mechanical full-thickness skin wounds and changed each other day as the experimental group,whereas the gauze full of saline were applied as the control group.The wound area of each wound was measured on the day of model made and the 3rd,7th,14th,28th day,thereafter,as well as the scores of inflammation response TNF-o and IL-6 levels in the wound tissues measured before and 3,7 days after model made.Results 7 days after treatment,the areas of the wound in the experimental group [(0.49 ± 0.08) cm2] was significantly smaller than that of the control group [(0.92 ± 0.11)cm2].The wounds in 2 groups were totally healed on the 28th day.On the 3rd,7th day after modeling,the score of inflammation response in the control group were about (4.51 ± 1.01) and (2.25 ± 0.62) respectively,whereas those of the experimental group were about (3.58 ± 0.51) and (1.66 ± 0.49) respectively.The differences of the two groups were statistically significant(t =2.85,2.59,all P ＜ 0.01).7 days after modeling,the amount of TNF-oin the tissues of the control group was (512 ±62)ng · L-1 · g-1,which was significantly higher than (434 ± 37) ng · L-1 · g-1 of the experimental group (t =3.74,P ＜ 0.05).The amount of IL-6 in the control group 3,7 days after modeling were (2 215 ±314)ng · L-1 · g-1 and (1 562 ± 174)ng · L-1 · g-1 respectively,which were significantly higher than (1672 ± 161)ng · L-1 · g-1 and (669 ±75)ng · L-1 · g-1 in the experimental group (t =5.33,16.33,all P ＜ 0.01).Conclusion Collagen sponge can intervene inflammatory reaction and promote the angiogenesis of the wound,promote wound healing.