中国基层医药
中國基層醫藥
중국기층의약
CHINESE JOURNAL OF PRIMARY MEDICINE AND PHARMACY
2014年
18期
2724-2726
,共3页
冷沉淀制备%时间%温度
冷沉澱製備%時間%溫度
랭침정제비%시간%온도
Cryoprecipitate%Time%Temperature
目的 探讨不同血浆制备温度和时间对冷沉淀质量的影响.方法 将新鲜冰冻血浆分成三个温度组通过虹吸法制备冷沉淀,比较融化时间和冷沉淀中FⅧ活性、Fg的含量.结果 2℃、4℃和6℃的融化时间分别为(96.3±5.9) min、(89.6±4.3) min、(81.2±2.7) min.温度越高,所需的融化时间越短.冷沉淀中FⅧ活性分别为(95.36±4.8)IU、(91.21±3.3)IU、(89.8±3.1)IU;冰冻血浆在2℃条件下融化所得冷沉淀中的FⅧ活性最高,与其他两组比较差异具有统计学意义(t=6.94,P=0.009,t =8.96,P=0.004),但4℃和6℃条件下融化所得冷沉淀中FⅧ活性差异无统计学意义(t=0.669,P=0.19).冷沉淀中Fg含量分别为(258.6±12.6)mg、(253.3±8.2)mg、(255.9±9.7) mg;不同血浆融化温度对Fg的含量没有显著影响,三组间两两比较差异无统计学意义(t=0.321,P=0.37;t =0.270,P=0.56;=0.424,P=0.29).结论 2℃条件下血浆融化所制备的冷沉淀中FⅧ活性最高,但是所需时间也最长;6℃条件下融化血浆所制备的冷沉淀FⅧ活性较低,但是符合质量要求,而且所需时间最短,冷沉淀中Fg含量三个温度组之间没有区别.血站可以采用6 ℃融化冰冻新鲜血浆来制备冷沉淀.
目的 探討不同血漿製備溫度和時間對冷沉澱質量的影響.方法 將新鮮冰凍血漿分成三箇溫度組通過虹吸法製備冷沉澱,比較融化時間和冷沉澱中FⅧ活性、Fg的含量.結果 2℃、4℃和6℃的融化時間分彆為(96.3±5.9) min、(89.6±4.3) min、(81.2±2.7) min.溫度越高,所需的融化時間越短.冷沉澱中FⅧ活性分彆為(95.36±4.8)IU、(91.21±3.3)IU、(89.8±3.1)IU;冰凍血漿在2℃條件下融化所得冷沉澱中的FⅧ活性最高,與其他兩組比較差異具有統計學意義(t=6.94,P=0.009,t =8.96,P=0.004),但4℃和6℃條件下融化所得冷沉澱中FⅧ活性差異無統計學意義(t=0.669,P=0.19).冷沉澱中Fg含量分彆為(258.6±12.6)mg、(253.3±8.2)mg、(255.9±9.7) mg;不同血漿融化溫度對Fg的含量沒有顯著影響,三組間兩兩比較差異無統計學意義(t=0.321,P=0.37;t =0.270,P=0.56;=0.424,P=0.29).結論 2℃條件下血漿融化所製備的冷沉澱中FⅧ活性最高,但是所需時間也最長;6℃條件下融化血漿所製備的冷沉澱FⅧ活性較低,但是符閤質量要求,而且所需時間最短,冷沉澱中Fg含量三箇溫度組之間沒有區彆.血站可以採用6 ℃融化冰凍新鮮血漿來製備冷沉澱.
목적 탐토불동혈장제비온도화시간대랭침정질량적영향.방법 장신선빙동혈장분성삼개온도조통과홍흡법제비랭침정,비교융화시간화랭침정중FⅧ활성、Fg적함량.결과 2℃、4℃화6℃적융화시간분별위(96.3±5.9) min、(89.6±4.3) min、(81.2±2.7) min.온도월고,소수적융화시간월단.랭침정중FⅧ활성분별위(95.36±4.8)IU、(91.21±3.3)IU、(89.8±3.1)IU;빙동혈장재2℃조건하융화소득랭침정중적FⅧ활성최고,여기타량조비교차이구유통계학의의(t=6.94,P=0.009,t =8.96,P=0.004),단4℃화6℃조건하융화소득랭침정중FⅧ활성차이무통계학의의(t=0.669,P=0.19).랭침정중Fg함량분별위(258.6±12.6)mg、(253.3±8.2)mg、(255.9±9.7) mg;불동혈장융화온도대Fg적함량몰유현저영향,삼조간량량비교차이무통계학의의(t=0.321,P=0.37;t =0.270,P=0.56;=0.424,P=0.29).결론 2℃조건하혈장융화소제비적랭침정중FⅧ활성최고,단시소수시간야최장;6℃조건하융화혈장소제비적랭침정FⅧ활성교저,단시부합질량요구,이차소수시간최단,랭침정중Fg함량삼개온도조지간몰유구별.혈참가이채용6 ℃융화빙동신선혈장래제비랭침정.
Objective To investigate the effects of temperature and time on the quality of cryoprecipitate.Methods Fresh frozen plasmas were divided into the three groups,and the cryoprecipitate were prepared from fresh frozen plasma by siphonage method thawing at 2℃,4℃ or 6℃ water bat.Thawing time,FⅧ and fibfinogen (Fg) were compared among the three groups.Results The thawing time at 2℃,4℃ or 6℃ was (96.3 ± 5.9) min,(89.6 ± 4.3) min,(81.2 ± 2.7) min.The activity of F Ⅷ was (95.36 ± 4.8) IU,(91.21 ± 3.3) IU,(89.8 ±3.1)IU,respectively.The activity of FⅧ was the highest at 2℃ condition and there was significantly different compared with other two groups (t =6.94,P =0.009,t =8.96,P =0.004).The activity of FⅧ in 4℃ group and 6℃ group were comparable (t =0.669,P =0.19).The concentration of Fg at three groups were similar (258.6 ±12.6) mg,(253.3 ±8.2)mg,(255.9 ±9.7) mg (t =0.321,P =0.37;t =0.270,P =0.56;t =0.424,P =0.29).Conclusion The activity of FⅧ at 2℃ condition were the highest but the thawing time was the longest.The activity of FⅧ at 6℃ condition were lower but met quality requirements and the thawing time was very short.There was no significantly different at Fg concentration among three groups.Thus 6℃ is more suitable for blood center to prepare cryoprecipitate from fresh frozen plasmas.
