CAJ | 학술논문

目的研究芹菜素对于转骨形态发生蛋白(BMP-7)软骨细胞修复关节软骨缺损的作用.方法 IL-1β体外诱导兔软骨细胞分泌白细胞介素-8(IL-8)和可溶性细胞黏附因子-1(sICAM-1).在培养系统中加入不同浓度芹菜素(10 μmol/L、25 μmol/L和50 μmol/L),采用酶联免疫吸附法检测芹菜素对诱导兔软骨细胞分泌IL-8和sICAM-1的影响.将软骨细胞种植于Matrigel胶支架上,将体外培养出的软骨膜块植入家兔关节软骨缺损模型中,采用随机数字表法分为3组(n=4):假手术组、转BMP-7组和转BMP-7+芹菜素组.5周后进行组织学观察和Wakitani评分.结果 10 μmol/L、25 μmol/L和50 μmol/L芹菜素能显著抑制IL-1β诱导兔软骨细胞分泌IL-8和sICAM-1的作用[(6803.63±162.31) ng/g、(6005.74±201.49) ng/g、(5202.34 ±271.67) ng/g比(10011.84±239.29)ng/g,P=0.00],并促进软骨细胞增殖.转BMP-7组和转BMP-7+芹菜素组的Wakitani评分显著低于假手术组[(3.68±0.86)比(8.25 ±0.90),P=0.00；(3.21 ±0.78)比(8.25 ±0.90),P=0.00].另外,还发现芹菜素可减少修复过程中的炎症反应发生.结论芹菜素可促进转BMP-7软骨细胞膜块构建以及关节软骨缺损的修复.
목적 연구근채소대우전골형태발생단백(BMP-7)연골세포수복관절연골결손적작용.방법 IL-1β체외유도토연골세포분비백세포개소-8(IL-8)화가용성세포점부인자-1(sICAM-1).재배양계통중가입불동농도근채소(10 μmol/L、25 μmol/L화50 μmol/L),채용매련면역흡부법검측근채소대유도토연골세포분비IL-8화sICAM-1적영향.장연골세포충식우Matrigel효지가상,장체외배양출적연골막괴식입가토관절연골결손모형중,채용수궤수자표법분위3조(n=4):가수술조、전BMP-7조화전BMP-7+근채소조.5주후진행조직학관찰화Wakitani평분.결과 10 μmol/L、25 μmol/L화50 μmol/L근채소능현저억제IL-1β유도토연골세포분비IL-8화sICAM-1적작용[(6803.63±162.31) ng/g、(6005.74±201.49) ng/g、(5202.34 ±271.67) ng/g비(10011.84±239.29)ng/g,P=0.00],병촉진연골세포증식.전BMP-7조화전BMP-7+근채소조적Wakitani평분현저저우가수술조[(3.68±0.86)비(8.25 ±0.90),P=0.00；(3.21 ±0.78)비(8.25 ±0.90),P=0.00].령외,환발현근채소가감소수복과정중적염증반응발생.결론 근채소가촉진전BMP-7연골세포막괴구건이급관절연골결손적수복.
Objective To study the effect of apigen on articular cartilage repair involving chondrocytes transfected with bone morphogenic protein-7 (BMP-7).Methods lnterleukin (IL-8) and soluble intercellular adhesion molecule-1 (sICAM-1) were induced by IL-1 β in rabbit chondrocytes.After apigen at different concentrations (10 μmol/L,25 μmol/L,and 50 μmol/L) was added into the culture system,the effect of apigen on IL8 and sICAM-1 production was observed using enzyme linked immunosorbent assay.Then chondrocytes were seeded on improved matrigel gel bracket and culturedin vitro to construct the compound,which was then transplanted into the rabbit model of articular cartilage defection.The rabbits were randomly divided into sham group (n =4),trans-BMP-7 group (n =4),and trans-BMP-7 + apigen group (n =4).Histological observation was conducted and Wakitani score calculated after 5 weeks.Results The concentrations of IL-8 and sICAM-1 in the chondrocytes supernatant in vitro significantly decreased after apigen treatment at 10 μmol/L,25 μmol/L,and 50 μmol/L [(6803.63 ±162.31) ng/g,(6005.74 ±201.49) ng/g,and (5202.34 ±271.67) ng/gvs.(10011.84±239.29) ng/g ; P =0.00].Five weeks after the cartilage cells on matrigel gel bracket were transplanted into rabbit models,the Wakitani scores of the trans-BMP-7 group and the trans-BMP-7 + apigen group were significantly lower than that of the sham group [(3.68 ± 0.86) vs.(8.25 ± 0.90),P =0.00 ; (3.21 ± 0.78) vs.(8.25 ±=0.90),P =0.00].In addition,no inflammatory reaction was noted during the repair in the trans-BMP-7 + apigen group.Conclusion Apigen can promote the construction of compound and repair of articular cartilage defects by trans-BMP-7 chondrocyte.