中华临床营养杂志
中華臨床營養雜誌
중화림상영양잡지
CHINESE JOURNAL OF CLINICAL NUTRITION
2013年
2期
90-97
,共8页
刘娟%刘艳华%计晨琳%侯淼%赵春玫%李晓南
劉娟%劉豔華%計晨琳%侯淼%趙春玫%李曉南
류연%류염화%계신림%후묘%조춘매%리효남
哺乳期过度喂养%高脂饮食%ω-3不饱和脂肪酸%脑肠肽生长激素释放肽%瘦素
哺乳期過度餵養%高脂飲食%ω-3不飽和脂肪痠%腦腸肽生長激素釋放肽%瘦素
포유기과도위양%고지음식%ω-3불포화지방산%뇌장태생장격소석방태%수소
Early over-feeding%High-fat diet%ω-3 polyunsaturated fatty acids%Ghrelin%Leptin
目的 观察哺乳期过度喂养大鼠断乳后给予ω-3脂肪酸,其胃组织中脑肠肽生长激素释放肽(ghrelin)表达和循环中瘦素(leptin)浓度的变化,探讨哺乳期过度喂养及断乳后不同饮食干预对成年期代谢综合征的影响及调控作用.方法 雄性SD大鼠出生后分为正常喂养组(NF组,每窝10只)和过度喂养组(OF组,每窝3只),出生3周后断乳,OF组分别给予正常饮食(OF+ Con)、高脂饮食(OF+ HF)及ω-3不饱和脂肪酸饮食(ω-3 PUFAs,OF+ω-3),NF组给予正常饮食(NF+Con)和高脂饮食(NF+ HF).每周记录体重及摄食量.6和16周时,进行糖耐量试验；酶联免疫吸附法分别测定各组血清leptin、ghrelin及甘油三酯(TG)浓度,实时定量PCR及免疫组织化学法分别测定各组胃组织ghrelin mRNA和蛋白表达浓度.结果 16周时,OF+ HF组大鼠能量摄入、体重明显高于NF+ HF组(t=-3.453,P=0.014; t=-6.406,P=0.000),血清TG高于OF+ Con组(t=4.72,P=0.005),30 min血糖浓度明显高于其他各组(F=16.249,P=0.000),血清leptin持续高于其他各组,胃组织ghrelin mRNA亦明显高于OF+ Con组(t=8.486,P=0.000),但胃组织ghrelin阳性细胞面积及血清ghrelin差异无统计学意义.16周时OF+ Con组血清leptin水平高于NF+ Con组(t=-3.274,P=0.031),OF+ HF组血清leptin水平高于OF+ Con组(t=3.028,P=0.014).OF+ω-3组上述指标与正常饮食组相比差异无统计学意义.结论 哺乳期过度喂养对高脂饮食诱导的肥胖和糖脂代谢紊乱更加敏感,更易发生肥胖、糖脂代谢失调,血清leptin和胃组织ghrelin mRNA表达持续增加,加重leptin抵抗和摄食调控的紊乱.ω-3 PUFAs能改善哺乳期过度喂养造成的脂肪组织过度积聚、糖耐量受损、leptin抵抗,维持正常的leptin和ghrelin浓度.
目的 觀察哺乳期過度餵養大鼠斷乳後給予ω-3脂肪痠,其胃組織中腦腸肽生長激素釋放肽(ghrelin)錶達和循環中瘦素(leptin)濃度的變化,探討哺乳期過度餵養及斷乳後不同飲食榦預對成年期代謝綜閤徵的影響及調控作用.方法 雄性SD大鼠齣生後分為正常餵養組(NF組,每窩10隻)和過度餵養組(OF組,每窩3隻),齣生3週後斷乳,OF組分彆給予正常飲食(OF+ Con)、高脂飲食(OF+ HF)及ω-3不飽和脂肪痠飲食(ω-3 PUFAs,OF+ω-3),NF組給予正常飲食(NF+Con)和高脂飲食(NF+ HF).每週記錄體重及攝食量.6和16週時,進行糖耐量試驗；酶聯免疫吸附法分彆測定各組血清leptin、ghrelin及甘油三酯(TG)濃度,實時定量PCR及免疫組織化學法分彆測定各組胃組織ghrelin mRNA和蛋白錶達濃度.結果 16週時,OF+ HF組大鼠能量攝入、體重明顯高于NF+ HF組(t=-3.453,P=0.014; t=-6.406,P=0.000),血清TG高于OF+ Con組(t=4.72,P=0.005),30 min血糖濃度明顯高于其他各組(F=16.249,P=0.000),血清leptin持續高于其他各組,胃組織ghrelin mRNA亦明顯高于OF+ Con組(t=8.486,P=0.000),但胃組織ghrelin暘性細胞麵積及血清ghrelin差異無統計學意義.16週時OF+ Con組血清leptin水平高于NF+ Con組(t=-3.274,P=0.031),OF+ HF組血清leptin水平高于OF+ Con組(t=3.028,P=0.014).OF+ω-3組上述指標與正常飲食組相比差異無統計學意義.結論 哺乳期過度餵養對高脂飲食誘導的肥胖和糖脂代謝紊亂更加敏感,更易髮生肥胖、糖脂代謝失調,血清leptin和胃組織ghrelin mRNA錶達持續增加,加重leptin牴抗和攝食調控的紊亂.ω-3 PUFAs能改善哺乳期過度餵養造成的脂肪組織過度積聚、糖耐量受損、leptin牴抗,維持正常的leptin和ghrelin濃度.
목적 관찰포유기과도위양대서단유후급여ω-3지방산,기위조직중뇌장태생장격소석방태(ghrelin)표체화순배중수소(leptin)농도적변화,탐토포유기과도위양급단유후불동음식간예대성년기대사종합정적영향급조공작용.방법 웅성SD대서출생후분위정상위양조(NF조,매와10지)화과도위양조(OF조,매와3지),출생3주후단유,OF조분별급여정상음식(OF+ Con)、고지음식(OF+ HF)급ω-3불포화지방산음식(ω-3 PUFAs,OF+ω-3),NF조급여정상음식(NF+Con)화고지음식(NF+ HF).매주기록체중급섭식량.6화16주시,진행당내량시험；매련면역흡부법분별측정각조혈청leptin、ghrelin급감유삼지(TG)농도,실시정량PCR급면역조직화학법분별측정각조위조직ghrelin mRNA화단백표체농도.결과 16주시,OF+ HF조대서능량섭입、체중명현고우NF+ HF조(t=-3.453,P=0.014; t=-6.406,P=0.000),혈청TG고우OF+ Con조(t=4.72,P=0.005),30 min혈당농도명현고우기타각조(F=16.249,P=0.000),혈청leptin지속고우기타각조,위조직ghrelin mRNA역명현고우OF+ Con조(t=8.486,P=0.000),단위조직ghrelin양성세포면적급혈청ghrelin차이무통계학의의.16주시OF+ Con조혈청leptin수평고우NF+ Con조(t=-3.274,P=0.031),OF+ HF조혈청leptin수평고우OF+ Con조(t=3.028,P=0.014).OF+ω-3조상술지표여정상음식조상비차이무통계학의의.결론 포유기과도위양대고지음식유도적비반화당지대사문란경가민감,경역발생비반、당지대사실조,혈청leptin화위조직ghrelin mRNA표체지속증가,가중leptin저항화섭식조공적문란.ω-3 PUFAs능개선포유기과도위양조성적지방조직과도적취、당내량수손、leptin저항,유지정상적leptin화ghrelin농도.
Objective To observe ghrelin expression in gastric tissue and serum leptin level of the early over-fed rats given ω-3 polyunsaturated fatty acids (PUFAs) diets after weaning,and explore the effects of early over-feeding and diets intervention on the metabolic syndrome in adult rats.Methods Male Sprague-Dawley rats were divided into normal feeding group (NF group,10 per litter) or over-feeding group (OF group,3 per litter) in postnatal day 3,and then different diets were given after weaning (postnatal day 21).The OF group was separately given conventional diet (OF + Con group),high-fat diet (OF + HF group),or ω-3 PUFAs (OF + ω-3 group) ; while the NF group was separated into NF + Con group and NF + HF group.Body weight and food intake were recorded every week.In week 6 and week 16,glucose tolerance test was perforfmed.Serum leptin,ghrelin,and triglyceride were assayed by enzyme-linked immuno-absorbent assay.Ghrelin mRNA and protein levels in gastric tissue were quantified by real-time PCR and immunohistochemistry.Results At week 16,energy intake,body weight and glucose intolerance in OF + HF group were significantly higher than in NF + HF group (t =-3.453,P =0.014 ; t =-6.406,P =0.000 ; F =16.249,P =0.000),and serum triglycerides,ghrelin mRNA of gastric tissue were significantly higher than those of OF + Con group (t =4.72,P =0.005 ; t =8.486,P =0.000).At week 16,the serum leptin level in OF + Con group was higher than that in NF + Con group (t =-3.274,P =0.031),also higher in OF + HF group than that in OF + Con group (t =3.028,P =0.014).There were no significant differences in serum ghrelin and the area of ghrelin immuno-positive cells in the gastric tissue among groups.The above indicators in OF + ω-3 group were not different from those of NF + Con group.Conclusions Over-feeding during the lactation period may lead to high susceptibility to obesity and disordered glucose and lipid metabolism.It can also increase serum leptin and ghrelin mRNA expression in gastric tissue,aggravate leptin resistance and feeding control disorders.Dietary ω-3 PUFAs offered protection against excessive accumulation of adipose tissue,glucose intolerance,leptin resistance,and maintained normal levels of leptin and ghrelin.
