CAJ | 학술논문

目的观察心力衰竭代偿期脂类代谢的变化,为心力衰竭的治疗提供依据.方法以微创手术小鼠主动脉弓狭窄模型作为研究对象,观察手术后小鼠不同时间点的动物整体脂肪含量的改变以及脂肪代谢有关基因的改变.结果主动脉弓狭窄术后3周小鼠体内脂肪的含量(0.49±0.13)g较假手术组(0.81±0.14)g显著降低(P =0.002);通过基因芯片分析代谢相关基因显示,心脏心钠肽的表达随着手术后存活时间的延长逐渐升高(R2=0.939,P=0.005),脂肪酸去饱和酶、长链脂肪酸延长酶5、N-脂酰鞘氨醇酰胺水解酶升高;脂肪酰辅酶A脱氢酶、肌糖原磷酸化酶、丙酮酸脱氢酶激酶降低,表现为脂肪动员增加而糖类利用降低.结论主动脉弓手术导致心脏复杂的代谢改变,激活了脂肪代谢,导致体内脂肪的动员,体脂含量减少.
목적 관찰심력쇠갈대상기지류대사적변화,위심력쇠갈적치료제공의거.방법 이미창수술소서주동맥궁협착모형작위연구대상,관찰수술후소서불동시간점적동물정체지방함량적개변이급지방대사유관기인적개변.결과 주동맥궁협착술후3주소서체내지방적함량(0.49±0.13)g교가수술조(0.81±0.14)g현저강저(P =0.002);통과기인심편분석대사상관기인현시,심장심납태적표체수착수술후존활시간적연장축점승고(R2=0.939,P=0.005),지방산거포화매、장련지방산연장매5、N-지선초안순선알수해매승고;지방선보매A탈경매、기당원린산화매、병동산탈경매격매강저,표현위지방동원증가이당류이용강저.결론 주동맥궁수술도치심장복잡적대사개변,격활료지방대사,도치체내지방적동원,체지함량감소.
Objective To observe the change of lipid metabolism during adaptive heart failure.Method The transverse aorta constriction mouse models established by minimal invasive surgery technique were evaluated for early small changes of body composition by EchoMRITM whole body composition analysis,and the different gene expression was analyzed by whole genome expression microarray.Results Three weeks after the surgery,the fat composition,in the mouse models were (0.49 ± 0.13) g,which was significantly lower than that in the sham surgery group [(0.81-±0.14) g] (P =0.002).The expression of the natriuretic peptide type A significantly increased over time (R2 =0.939,P =0.005).In addition,the levels of fatty acid desaturase,long-chain fatty acid elongase 5,and N-acylsphingosine amidohydrolase increased,and the levels of acyl-coenzyme A dehydrogenase,muscle glycogen phosphorylase,and pyruvate dehydrogenase kinase decreased,showing increased fat mobilization and decreased carbohydrate utilization.Conclusions Surgery for transverse aorta constriction can cause complex metablic changes in heart.The activated lipid mobilization maybe lead to the low body fat composition.