目的 研究测定肠道黏膜屏障功能的几种方法及评价不同方法之间的相关性.方法 16只无特定病原体动物(SPF)级雄性SD大鼠,随机分为对照组(Control)和缺血/再灌注(L/R)组,每组8只.适应性饲养5d后,I/R组大鼠进行肠道缺血60 min,Control组只开腹60 min,然后继续饲养1d后取材.检测两组血浆二胺氧化酶(DAO)、D-乳酸(D-LAC)、内毒素、谷氨酰胺(Gln)的浓度,观察小肠黏膜形态学.术前1d和术后1d分别测定肠道通透性乳果糖/甘露醇(L/M).结果 I/R组血浆DAO、D-LAC和内毒素水平显著高于Control组[DAO:(0.498±0.032) U/ml比(0.247±0.051) U/ml,t=-11.790,P=0.000; D-LAC:(5.47±1.55) mg/L比(3.83 ±0.63) mg/L,t=-2.757,P=0.022;内毒素:(0.039 5±0.002 8) EU/ml比(0.025 6±0.004 5) EU/ml,t=-7.377,P=0.000];血浆Gln浓度显著低于Control组,为(646.12±34.75)μmol/L比(839.13±163.76) μmol/L(t=3.261,P=0.012);I/R组术后1 dL/M值明显高于术前1d,为3.63±2.09比1.22±0.66(t=-3.118,P=0.013).术后I/R组的空肠黏膜厚度、空肠绒毛高度、回肠黏膜厚度和回肠绒毛高度明显低于Control组,分别为(329.80±64.68) μm比(512.82±38.41)μm (t=6.881,P=0.000),(253.06±69.33) μm比(386.79 ±56.39) μm(t=4.232,P=0.001),(205.89±18.71) μm比(335.29±27.71) μm (t=10.945,P=0.000),(135.61±22.30) μm比(253.18 ±31.02) μm(t=8.705,P=0.000).I/R组肠道缺血/再灌注后血浆DAO、D-LAC、内毒素及L/M均增高,DAO与D-LAC(r=0.971,P＜0.01)、内毒素(r=0.915,P＜0.01)及L/M(r=0.957,P＜0.01)相互之间呈正相关;缺血/再灌注后血浆Gln浓度降低,与DAO(r=-0.964,P＜0.01)、D-LAC(r=-0.966,P＜0.01)、内毒素(r=-0.927,P＜0.01)和L/M(r=-0.993,P＜0.01)分别呈负相关.结论 肠道缺血/再灌注后,各项指标均有明显改变并具有良好的相关性,DAO与D-LAC、内毒素及L/M间呈显著正相关,与肠道屏障功能呈负相关.Gln和小肠黏膜形态之间呈正相关且分别与其他几项指标呈显著负相关.
目的 研究測定腸道黏膜屏障功能的幾種方法及評價不同方法之間的相關性.方法 16隻無特定病原體動物(SPF)級雄性SD大鼠,隨機分為對照組(Control)和缺血/再灌註(L/R)組,每組8隻.適應性飼養5d後,I/R組大鼠進行腸道缺血60 min,Control組隻開腹60 min,然後繼續飼養1d後取材.檢測兩組血漿二胺氧化酶(DAO)、D-乳痠(D-LAC)、內毒素、穀氨酰胺(Gln)的濃度,觀察小腸黏膜形態學.術前1d和術後1d分彆測定腸道通透性乳果糖/甘露醇(L/M).結果 I/R組血漿DAO、D-LAC和內毒素水平顯著高于Control組[DAO:(0.498±0.032) U/ml比(0.247±0.051) U/ml,t=-11.790,P=0.000; D-LAC:(5.47±1.55) mg/L比(3.83 ±0.63) mg/L,t=-2.757,P=0.022;內毒素:(0.039 5±0.002 8) EU/ml比(0.025 6±0.004 5) EU/ml,t=-7.377,P=0.000];血漿Gln濃度顯著低于Control組,為(646.12±34.75)μmol/L比(839.13±163.76) μmol/L(t=3.261,P=0.012);I/R組術後1 dL/M值明顯高于術前1d,為3.63±2.09比1.22±0.66(t=-3.118,P=0.013).術後I/R組的空腸黏膜厚度、空腸絨毛高度、迴腸黏膜厚度和迴腸絨毛高度明顯低于Control組,分彆為(329.80±64.68) μm比(512.82±38.41)μm (t=6.881,P=0.000),(253.06±69.33) μm比(386.79 ±56.39) μm(t=4.232,P=0.001),(205.89±18.71) μm比(335.29±27.71) μm (t=10.945,P=0.000),(135.61±22.30) μm比(253.18 ±31.02) μm(t=8.705,P=0.000).I/R組腸道缺血/再灌註後血漿DAO、D-LAC、內毒素及L/M均增高,DAO與D-LAC(r=0.971,P＜0.01)、內毒素(r=0.915,P＜0.01)及L/M(r=0.957,P＜0.01)相互之間呈正相關;缺血/再灌註後血漿Gln濃度降低,與DAO(r=-0.964,P＜0.01)、D-LAC(r=-0.966,P＜0.01)、內毒素(r=-0.927,P＜0.01)和L/M(r=-0.993,P＜0.01)分彆呈負相關.結論 腸道缺血/再灌註後,各項指標均有明顯改變併具有良好的相關性,DAO與D-LAC、內毒素及L/M間呈顯著正相關,與腸道屏障功能呈負相關.Gln和小腸黏膜形態之間呈正相關且分彆與其他幾項指標呈顯著負相關.
목적 연구측정장도점막병장공능적궤충방법급평개불동방법지간적상관성.방법 16지무특정병원체동물(SPF)급웅성SD대서,수궤분위대조조(Control)화결혈/재관주(L/R)조,매조8지.괄응성사양5d후,I/R조대서진행장도결혈60 min,Control조지개복60 min,연후계속사양1d후취재.검측량조혈장이알양화매(DAO)、D-유산(D-LAC)、내독소、곡안선알(Gln)적농도,관찰소장점막형태학.술전1d화술후1d분별측정장도통투성유과당/감로순(L/M).결과 I/R조혈장DAO、D-LAC화내독소수평현저고우Control조[DAO:(0.498±0.032) U/ml비(0.247±0.051) U/ml,t=-11.790,P=0.000; D-LAC:(5.47±1.55) mg/L비(3.83 ±0.63) mg/L,t=-2.757,P=0.022;내독소:(0.039 5±0.002 8) EU/ml비(0.025 6±0.004 5) EU/ml,t=-7.377,P=0.000];혈장Gln농도현저저우Control조,위(646.12±34.75)μmol/L비(839.13±163.76) μmol/L(t=3.261,P=0.012);I/R조술후1 dL/M치명현고우술전1d,위3.63±2.09비1.22±0.66(t=-3.118,P=0.013).술후I/R조적공장점막후도、공장융모고도、회장점막후도화회장융모고도명현저우Control조,분별위(329.80±64.68) μm비(512.82±38.41)μm (t=6.881,P=0.000),(253.06±69.33) μm비(386.79 ±56.39) μm(t=4.232,P=0.001),(205.89±18.71) μm비(335.29±27.71) μm (t=10.945,P=0.000),(135.61±22.30) μm비(253.18 ±31.02) μm(t=8.705,P=0.000).I/R조장도결혈/재관주후혈장DAO、D-LAC、내독소급L/M균증고,DAO여D-LAC(r=0.971,P＜0.01)、내독소(r=0.915,P＜0.01)급L/M(r=0.957,P＜0.01)상호지간정정상관;결혈/재관주후혈장Gln농도강저,여DAO(r=-0.964,P＜0.01)、D-LAC(r=-0.966,P＜0.01)、내독소(r=-0.927,P＜0.01)화L/M(r=-0.993,P＜0.01)분별정부상관.결론 장도결혈/재관주후,각항지표균유명현개변병구유량호적상관성,DAO여D-LAC、내독소급L/M간정현저정상관,여장도병장공능정부상관.Gln화소장점막형태지간정정상관차분별여기타궤항지표정현저부상관.
Objective To study several measuring methods of the intestinal mucosa barrier and evaluate the correlation between different methods for determining the intestinal damage.Methods Sixteen specific pathogen free (SPF) male Sprague-Dawley rats were randomly divided into two groups:the Control group (n =8) and the ischemia/reperfusion (I/R) group (n =8).After adaptive feeding for 5 days,I/R group was put into ischemia model for 60 min and the Control group was merely opened on its abdominal wall but without ischemia for 60 min.After having been fed for another day,all rats were killed and specimens were collected.The plasma diamine oxidase (DAO),D-lactate (D-LAC),endotoxin,and glutamine (Gln) levels were detected,and the intestinal mucosal morphology was observed.The intestinal permeability (L/M) was detected 1 day before and after the surgery.Results The plasma DAO,D-LAC,and endotoxin levels were significantly higher in I/R group compared with the Control group (DAO:(0.498 ±0.032) vs (0.247 ±0.051) U/ml,t=-11.790,P=0.000; D-LAC:(5.47±1.55) vs (3.83±0.63) mg/L,t=-2.757,P=0.022; endotoxin:(0.0395±0.002 8) vs (0.025 6 ±0.004 5) EU/ml,t =-7.377,P =0.000).The plasma Gln concentration was significantly lower than that in the Control group [(646.12 ± 34.75) vs (839.13 ± 163.76) μmol/L,t =3.261,P =0.012).The L/M value on the 1 st postoperative day was significantly higher than that in the I/R group [(3.63 ±2.09) vs (1.22 ±0.66),t =-3.118,P =0.013)].The jejunum mucosal thickness,jejunum villus height,ileal mucosal thickness,and ileal villus height were significantly lower in I/R group compared with the Control group after operation [(329.80 ±64.68) vs (512.82 ±38.41) μm,t=6.881,P=0.000; (253.06±69.33) vs (386.79±56.39) μm,t=4.232,P=0.001; (205.89± 18.71) vs (335.29±27.71) μm,t=10.945,P=0.000; (135.61 ±22.30) vs (253.18±31.02) μm,t =8.705,P =0.000].After intestinal ischemia/reperfusion,DAO,D-LAC,endotoxin and L/M were all increased and positively correlated with each other.The plasma concentration of Gln and the morphological changes of jejunum and ileum were negatively correlated with DAO,D-LAC,endotoxin,and L/M,respectively.Conclusions After intestinal ischemia/rcperfusion,the levels of all examination indicators obviously change and correlate with each other.The DAO,D-LAC,endotoxin,and L/M are positively related to each other and negatively correlated with the intestinal barrier function.Gln is positively correlated with small intestinal mucosal morphology and negatively correlated with others,respectively.
