CAJ | 학술논문

目的研究应用人参皂甙Rg1球周注射对视神经夹伤大鼠组织病理的影响.方法将SD大鼠随机分成两组,A组12只(24只眼),右眼不做任何处理作为对正常对照组,左眼行视神经夹伤作为损伤对照组.B组12只(24只眼),双眼均行视神经夹伤,右眼注射等体积生理盐水作为治疗对照组,左眼球周注射人参皂甙Rg1(每只眼2 mg/0.1 ml),1次/d,共21 d,作为实验组.用手术显微镜观察各组眼部临床症状学病程变化,用视网膜神经节细胞(RGCs)特异性标记物转录因子Brn-3a标记RGCs,分别于造模成功后3、7、14、28 d进行视网膜组织病理、免疫组织化学染色,比较各组之间差异.结果 (1)视神经钳夹伤3～7 d,损伤组、生理盐水组RGCs单层排列结构消失,组织细胞排列疏松、增厚;实验组视网膜水肿较以上两组程度减轻.(2) 3～28 d各时间点RGCs进行性丢失,细胞数目逐渐减少,晚期阶段视网膜神经节细胞层(GCL)可见空泡化改变;实验组也可见上述改变,但程度较损伤与生理盐水对照组轻.(3)损伤组与生理盐水组RGCs存活数量差异无统计学意义(P＞0.05);但实验组RGCs存活数量显著高于同时期生理盐水组对照组,差异具有统计学意义(P＜0.05).结论球周注射人参皂甙Rg1在视神经损伤的病理条件下能够维持眼部与视网膜组织的正常形态结构,提高钳夹伤后RGCs的存活率,减少手术损伤并发症.提示本方法对视神经钳夹伤大鼠的治疗作用安全、有效,有可能用于临床上视神经损伤疾病的治疗.
목적 연구응용인삼조대Rg1구주주사대시신경협상대서조직병리적영향.방법 장SD대서수궤분성량조,A조12지(24지안),우안불주임하처리작위대정상대조조,좌안행시신경협상작위손상대조조.B조12지(24지안),쌍안균행시신경협상,우안주사등체적생리염수작위치료대조조,좌안구주주사인삼조대Rg1(매지안2 mg/0.1 ml),1차/d,공21 d,작위실험조.용수술현미경관찰각조안부림상증상학병정변화,용시망막신경절세포(RGCs)특이성표기물전록인자Brn-3a표기RGCs,분별우조모성공후3、7、14、28 d진행시망막조직병리、면역조직화학염색,비교각조지간차이.결과 (1)시신경겸협상3～7 d,손상조、생리염수조RGCs단층배렬결구소실,조직세포배렬소송、증후;실험조시망막수종교이상량조정도감경.(2) 3～28 d각시간점RGCs진행성주실,세포수목축점감소,만기계단시망막신경절세포층(GCL)가견공포화개변;실험조야가견상술개변,단정도교손상여생리염수대조조경.(3)손상조여생리염수조RGCs존활수량차이무통계학의의(P＞0.05);단실험조RGCs존활수량현저고우동시기생리염수조대조조,차이구유통계학의의(P＜0.05).결론 구주주사인삼조대Rg1재시신경손상적병리조건하능구유지안부여시망막조직적정상형태결구,제고겸협상후RGCs적존활솔,감소수술손상병발증.제시본방법대시신경겸협상대서적치료작용안전、유효,유가능용우림상상시신경손상질병적치료.
Objective To make a study on the histopathological influence of ginsenosides Rg1 periocular injection after optic nerve crush in rats.Method SD rats were randomly divided into two groups,A group of 12 (24 eyes),the right eye without any treatment as a normal control group,left eye made optic nerve crush as the control group.Group B,12 (24 eyes),eyes underwent optic nerve crush,right eye injected with saline as a treatment control group,the left eye injected with ginsenosides Rg1 (2mg/0.1ml / eyes) as the experimental group.Clinical symptomatology changes of each group were observed with a surgical microscope,specific markers transcription factor Brn-3a labeled retinal ganglion cells (RGCs),pathology and immunohistochemistry were examined at 3,7,14,28 d after injury,respectively.Differences between groups were compared.Results (1) at 3-7d after optic crush,monolayer structure of injury and saline group disappeared,tissue loosely arranged thickening,retinal edema of the experimental group lessened compared with that of the two sets.(2)progressive loss of RGCs were observed at 3～28d,the number of cells reduced gradually,vacuoles appeared at the late stage.These changes were also seen in experimental group and degree of injury was lighter than those of two groups.(3)There was no significant difference between the survival in injury and saline group (P ＞0.05); survival number of RGCs in experimental group was significantly higher than that of saline control group,the difference was statistically significant(P ＜0.05).Conclusions Rg1 ginsenosides Rg1 periocular injection can maintain the normal morphology structure of optic retinal tissue,improve the survival rate after nerve crush damage.It hints that this method is safe,effective and may be used in clinical optic nerve diseases.