中国实用眼科杂志
中國實用眼科雜誌
중국실용안과잡지
CHINESE JOURNAL OF PRACTICAL OPHTHALMOLOGY
2014年
5期
599-603
,共5页
视网膜母细胞瘤%免疫逃逸%CD4+CD25+Treg细胞
視網膜母細胞瘤%免疫逃逸%CD4+CD25+Treg細胞
시망막모세포류%면역도일%CD4+CD25+Treg세포
Retinoblastoma%Immune escape%CD4+CD25+Treg
目的 探讨CD4+ CD25+ Treg在视网膜母细胞瘤(retinoblastoma,RB)发生免疫逃逸中的作用.方法 收集2007年1～12月在中山眼科中心诊治的RB患者31例.临床资料,设立实验组与对照组.将RB患者作为实验组,共31例;阴性对照组:Coats病患儿1例及眼球破裂伤患儿7例.以流式细胞仪检测患者外周血及组织局部CD4+ CD25+ Treg的比例.又以同样方法检测了7例眼睑恶性肿瘤、11例泪腺恶性肿瘤、13例泪腺良性肿瘤及8例视神经良性肿瘤患者肿瘤局部及外周血中CD4+CD25+ Treg的比例.结果 RB患者肿瘤局部较阴性对照组组织局部CD4+ CD25+ Treg的比例明显增高,分别为(21.56±6.83)％、(9.33±4.45)％,2个组差异具有统计学意义(t=4.51,P=0.032);2个组患者外周血中CD4+ CD25+ Treg的比例分别为(9.51±3.98)％、(8.48±3.43)％,差异无统计学意义(t =0.079,P=0.217).眼眶恶性肿瘤(眼睑、泪腺恶性肿瘤)局部CD4+ CD25+ Treg比例均较高,分别为(23.62±8.62)％、(29.74±9.22)％,而眼眶良性肿瘤(泪腺、视神经良性肿瘤)局部CD4+ CD25+ Treg比例则较低,分别为(12.61±5.96)％、(8.03±3.57)％,无论眼眶恶性肿瘤还是良性肿瘤患者外周血中CD4+ CD25+ Treg比例较正常人群均无明显增高.结论 RB患者肿瘤局部CD4+CD25+ Treg比例增高可能是引起视网膜母细胞瘤肿瘤局部发生免疫逃逸的原因之一.
目的 探討CD4+ CD25+ Treg在視網膜母細胞瘤(retinoblastoma,RB)髮生免疫逃逸中的作用.方法 收集2007年1～12月在中山眼科中心診治的RB患者31例.臨床資料,設立實驗組與對照組.將RB患者作為實驗組,共31例;陰性對照組:Coats病患兒1例及眼毬破裂傷患兒7例.以流式細胞儀檢測患者外週血及組織跼部CD4+ CD25+ Treg的比例.又以同樣方法檢測瞭7例眼瞼噁性腫瘤、11例淚腺噁性腫瘤、13例淚腺良性腫瘤及8例視神經良性腫瘤患者腫瘤跼部及外週血中CD4+CD25+ Treg的比例.結果 RB患者腫瘤跼部較陰性對照組組織跼部CD4+ CD25+ Treg的比例明顯增高,分彆為(21.56±6.83)％、(9.33±4.45)％,2箇組差異具有統計學意義(t=4.51,P=0.032);2箇組患者外週血中CD4+ CD25+ Treg的比例分彆為(9.51±3.98)％、(8.48±3.43)％,差異無統計學意義(t =0.079,P=0.217).眼眶噁性腫瘤(眼瞼、淚腺噁性腫瘤)跼部CD4+ CD25+ Treg比例均較高,分彆為(23.62±8.62)％、(29.74±9.22)％,而眼眶良性腫瘤(淚腺、視神經良性腫瘤)跼部CD4+ CD25+ Treg比例則較低,分彆為(12.61±5.96)％、(8.03±3.57)％,無論眼眶噁性腫瘤還是良性腫瘤患者外週血中CD4+ CD25+ Treg比例較正常人群均無明顯增高.結論 RB患者腫瘤跼部CD4+CD25+ Treg比例增高可能是引起視網膜母細胞瘤腫瘤跼部髮生免疫逃逸的原因之一.
목적 탐토CD4+ CD25+ Treg재시망막모세포류(retinoblastoma,RB)발생면역도일중적작용.방법 수집2007년1～12월재중산안과중심진치적RB환자31례.림상자료,설립실험조여대조조.장RB환자작위실험조,공31례;음성대조조:Coats병환인1례급안구파렬상환인7례.이류식세포의검측환자외주혈급조직국부CD4+ CD25+ Treg적비례.우이동양방법검측료7례안검악성종류、11례루선악성종류、13례루선량성종류급8례시신경량성종류환자종류국부급외주혈중CD4+CD25+ Treg적비례.결과 RB환자종류국부교음성대조조조직국부CD4+ CD25+ Treg적비례명현증고,분별위(21.56±6.83)％、(9.33±4.45)％,2개조차이구유통계학의의(t=4.51,P=0.032);2개조환자외주혈중CD4+ CD25+ Treg적비례분별위(9.51±3.98)％、(8.48±3.43)％,차이무통계학의의(t =0.079,P=0.217).안광악성종류(안검、루선악성종류)국부CD4+ CD25+ Treg비례균교고,분별위(23.62±8.62)％、(29.74±9.22)％,이안광량성종류(루선、시신경량성종류)국부CD4+ CD25+ Treg비례칙교저,분별위(12.61±5.96)％、(8.03±3.57)％,무론안광악성종류환시량성종류환자외주혈중CD4+ CD25+ Treg비례교정상인군균무명현증고.결론 RB환자종류국부CD4+CD25+ Treg비례증고가능시인기시망막모세포류종류국부발생면역도일적원인지일.
Objective To investigate the effect of CD4+CD25+ Regulatory T cell in the tumor immune escape mechanism in patients with retinoblastoma.Methods Flow cytometric analysis was used to quantify the prevalence of CD4+CD25+ Regulatory T cell in the peripheral blood and fresh tissue samples from the patients with retinoblastoma (31 eyes).Peripheral blood samples and retina tissue were also collected from the patients with coats disease (one eye) and the patients with eyeball rupture (7 eyes) for comparison.Moreover,the same method was used to quantify the prevalence of CD4+CD25+ Regulatory T cell in the peripheral blood and fresh tissue samples from the patients with eyelid malignant tumor (7 eyes),malignant tumor of lacrimal gland (11 eyes),benign tumor of lacrimal gland (13 eyes),and benign tumor of the optic nerve (8 eyes).Results The percentages of CD4+CD25+ Regulatory T cell in the tumor sample of the patients with retinoblastoma were significantly higher than that of control group (21.56±6.83％ and 9.33±4.45％ respectively,t =4.51,P =0.032).Compared with control group,the proportion of CD4+CD25+ Regulatory T cell in peripheral blood of patients with retinoblastoma,there was no significant difference (9.51±3.98％ and 8.48±3.43％ respectively,t =0.019,P =0.217).The percentages of CD4+CD25+ Regulatory T cell in the tumor sample of the patients with eyelid malignant tumor and malignant tumor of lacrimal gland was higher (23.62±8.62％ and 29.74±9.22％ respectively),and which in the patients with lacrimal gland benign tumor and the optic nerve benign tumor was lower (12.61±5.96％ and 8.03±3.57％,respectively).Compared with the normal people,there was no significant increased in the proportion of CD4+CD25+ Regulatory T cell in peripheral blood of patients with malignant tumor or benign tumor of orbit.Conclusions This research show that the expression of CD4+CD25+ Regulatory T cell is increased in the local tumor micro-environment of the patients with retinoblastomal.That may be one factor of the mechanism of tumor immune escape.
