CAJ | 학술논문

目的探讨2型糖尿病(T2DM)患者血清8-羟基脱氧鸟苷酸(8-OHdG)和糖尿病性视网膜病变的关系.方法对2012年12月至2013年3月在广东医学院附属医院眼科住院的49例T2DM患者根据视网膜损害的不同,分成无糖尿病性视网膜病变组(DM组,12例)、非增殖性糖尿病性视网膜病变组(NPDR组,19例)和增殖性糖尿病性视网膜病变组(PDR组,18例),采用酶联免疫吸附法(ELISA)测定其血清8-OHdG水平,并与健康体检者(NC组,22人)进行比较.结果 T2DM组和对照组8-0HdG水平不满足正态分布及方差齐性,用Willcoxon W秩和检验进行两组比较,差异有统计学意义(Z =-6.70,P＜0.01),T2DM组8-0HdG水平(平均秩和为47.00)高于对照组(平均秩和为11.50);Kruskal-Wallis秩和检验法比较T2DM 3个亚组间血清8-0HdG水平差异有统计学意义(x2=59.50,P=0.00),PDR组8-OHdG水平最高(平均秩和为38.08),NPDR组次之(平均秩和为22.68),DM组最低(平均秩和为9.04).Spearman相关分析表明T2DM患者血清8-0HdG与糖尿病性视网膜病变程度呈正相关(r =0.94,P=0.00).结论 T2DM患者的血清8-OHdG水平的增高与糖尿病性视网膜病变有关,提示血清8-OHdG水平增高是T2DM患者合并糖尿病性视网膜病变的危险因素之一.
목적 탐토2형당뇨병(T2DM)환자혈청8-간기탈양조감산(8-OHdG)화당뇨병성시망막병변적관계.방법 대2012년12월지2013년3월재엄동의학원부속의원안과주원적49례T2DM환자근거시망막손해적불동,분성무당뇨병성시망막병변조(DM조,12례)、비증식성당뇨병성시망막병변조(NPDR조,19례)화증식성당뇨병성시망막병변조(PDR조,18례),채용매련면역흡부법(ELISA)측정기혈청8-OHdG수평,병여건강체검자(NC조,22인)진행비교.결과 T2DM조화대조조8-0HdG수평불만족정태분포급방차제성,용Willcoxon W질화검험진행량조비교,차이유통계학의의(Z =-6.70,P＜0.01),T2DM조8-0HdG수평(평균질화위47.00)고우대조조(평균질화위11.50);Kruskal-Wallis질화검험법비교T2DM 3개아조간혈청8-0HdG수평차이유통계학의의(x2=59.50,P=0.00),PDR조8-OHdG수평최고(평균질화위38.08),NPDR조차지(평균질화위22.68),DM조최저(평균질화위9.04).Spearman상관분석표명T2DM환자혈청8-0HdG여당뇨병성시망막병변정도정정상관(r =0.94,P=0.00).결론 T2DM환자적혈청8-OHdG수평적증고여당뇨병성시망막병변유관,제시혈청8-OHdG수평증고시T2DM환자합병당뇨병성시망막병변적위험인소지일.
Objective To investigate the relationship of the levels of serum 8-hydroxydeoxyguanosin (8-OHdG) to diabetic retinopathy (DR) in subjects with type 2 diabetes (T2DM).Methods The patients were classified into groups according to retinopathy (no signs of diabetic retinopathy [DM],nonproliferative diabetic retinopathy [NPDR],and proliferative diabetic retinopathy [PDR]).The concentration of serum 8-OHdG were measured with enzyme-linked immunosorbent assay (ELISA) in all 49 patients with diabetes mellitus and 22 normal control subjects.The correlation between 8-OHdG and diabetic retinopathy were analyzed in all patients with T2DM.Results The concentration of serum 8-OHdG in patients with T2DM were significantly higher than those in the control subjects (Willcoxon W Test,Z =-6.70,P =0.00; Mean Rank of T2DM group and normal control subjects are 47.00 and 11.50 respectively).There was statistical difference in the levels of serum 8-OHdG in the PDR,NPDR,and DM groups (Kruskal Wallis Test,x2=59.50,P =0.00).The levels of serum 8-OHdG was much higher in the patients with PDR than that in patients with diabetes without retinopathy and with NPDR,The concentration of serum 8-OHdG was higher in the patients with NPDR than that in patients with diabetes without retinopathy.The degree of 8-OHdG was correlated with the degree of DR (Spearman correlation coefficients=0.94,P =0.00).Conclusions The elevated serum 8-OHdG may be one of probable risk factors in the diabetic retinopathy in the patients with T2DM.