CAJ | 학술논문

目的通过检测P-gp、GST-π和LRP在眼眶淋巴瘤组织芯片中的表达情况,从耐药角度探索该病的复发及预后不良机制.方法收集天津市眼科医院及天津市第一中心医院眼科于2006年9月至2011年6月经手术切除且病理诊断明确的50例(其中46例为观察组)眼眶淋巴瘤石蜡标本,另取眼眶反应性淋巴增生17例作为对照组.制作眼眶淋巴瘤组织芯片,应用免疫组织化学技术检测P-gp、GST-π、LRP在眼眶淋巴瘤中的表达.并对46例眼眶淋巴瘤病人预后进行随访,以比较不同预后患者淋巴瘤组织中P-gp、GST-π、LRP的表达情况.结果 P-gp在眼眶淋巴瘤和反应性淋巴增生组织芯片中的阳性表达率分别为87.0％ (40/46)和35.3％ (6/17),其差异有统计学意义(x2=16.81,P=0.000):GST-π在以上两种病变的阳性表达率分别为76.1％ (35/46)和41.2％ (7/17),其差异有统计学意义(x2=6.808,P=0.009); LRP在两种组织中均为阴性表达.在眼眶淋巴瘤预后不良和预后良好之间,P-gp的阳性表达率分别为85.7％ (12/14)和42.9％ (9/21),经Fisher检验,P=0.033,其差异有统计学意义;GST-π在以上两种情况间的阳性表达率分别为78.6％ (11/14)和38.1％ (8/21),经Fisher检验,P=0.013,其差异有统计学意义.结论眼眶淋巴瘤组织芯片省时快速、高效经济地研究了该肿瘤的多药耐药基因表达状况.眼眶淋巴瘤的多药耐药性与P-gp和GST-π有关.
목적 통과검측P-gp、GST-π화LRP재안광림파류조직심편중적표체정황,종내약각도탐색해병적복발급예후불량궤제.방법 수집천진시안과의원급천진시제일중심의원안과우2006년9월지2011년6월경수술절제차병리진단명학적50례(기중46례위관찰조)안광림파류석사표본,령취안광반응성림파증생17례작위대조조.제작안광림파류조직심편,응용면역조직화학기술검측P-gp、GST-π、LRP재안광림파류중적표체.병대46례안광림파류병인예후진행수방,이비교불동예후환자림파류조직중P-gp、GST-π、LRP적표체정황.결과 P-gp재안광림파류화반응성림파증생조직심편중적양성표체솔분별위87.0％ (40/46)화35.3％ (6/17),기차이유통계학의의(x2=16.81,P=0.000):GST-π재이상량충병변적양성표체솔분별위76.1％ (35/46)화41.2％ (7/17),기차이유통계학의의(x2=6.808,P=0.009); LRP재량충조직중균위음성표체.재안광림파류예후불량화예후량호지간,P-gp적양성표체솔분별위85.7％ (12/14)화42.9％ (9/21),경Fisher검험,P=0.033,기차이유통계학의의;GST-π재이상량충정황간적양성표체솔분별위78.6％ (11/14)화38.1％ (8/21),경Fisher검험,P=0.013,기차이유통계학의의.결론 안광림파류조직심편성시쾌속、고효경제지연구료해종류적다약내약기인표체상황.안광림파류적다약내약성여P-gp화GST-π유관.
Objective To explore the recurrence and poor prognosis mechanism from the perspective multi-drug resistance (MDR) by detecting the expression of P-gp,GST-π and LRP in the orbital lymphoma.Methods Fifty orbital lymphoma tissue which were diagnosed by pathology after surgery were collected from Tianjin eye hospital and Tianjin first centre hospital.Among them,46 cases were the observation group.Meanwhile,17 reactive lymphoid hyperplasia tissues were collected as contrast group.Orbital lymphoma tissue microarray and immunohistochemical staining was used to detect the expression of P-gp,GST-π and LRP in orbital lymphoma and reactive lymphoid hyperplasia.Results The P-gp expression rates were 87.0％(40/46) and 35.3％(6/17) in the two tumors tissue microarray and there was significant difference between them (x2=16.81,P =0.000).The GST-π expression rates were 76.1％(35/46) and 41.2％(7/17) in the two tumors tissue microarray and there was significant difference between them (x2=6.808,P =0.009).In poor prognosis group and good prognosis group of the orbital lymphoma,the P-gp expression rates were 85.7％(12/14) and 42.9％(9/21) and there was significant difference between them (Fisher test,P =0.033).The GST-π expression rates were 78.6％(11/14) and 38.1％ (8/21) in the two groups and there was significant difference between them (Fisher test,P=0.013).Conclusions By orbital lymphoma tissue microarray,the MDR gene expression can be detected rapidly,it is efficiently and economically.The MDR of orbital lymphoma is associated with the big expression of P-gp and GST-π.