中华胃肠外科杂志
中華胃腸外科雜誌
중화위장외과잡지
CHINESE JOURNAL OF GASTROINTESTINAL SURGERY
2012年
11期
1146-1149
,共4页
谢杰斌%蔡景理%李立军%张林君%姜明哲%陈登刚%陈荣
謝傑斌%蔡景理%李立軍%張林君%薑明哲%陳登剛%陳榮
사걸빈%채경리%리립군%장림군%강명철%진등강%진영
2型糖尿病%胃旁路术%磷酸烯醇式丙酮酸羧激酶%Goto-Kakizaki大鼠
2型糖尿病%胃徬路術%燐痠烯醇式丙酮痠羧激酶%Goto-Kakizaki大鼠
2형당뇨병%위방로술%린산희순식병동산최격매%Goto-Kakizaki대서
Type 2 diabetes mellitus%Gastric bypass%Phosphoenolpyruvate carboxykinase%Goto-Kakizaki rat
目的 研究胃旁路术对2型糖尿病Goto-Kakizaki(GK)大鼠降糖作用及肝脏磷酸烯醇式丙酮酸羧激酶(PEPCK)mRNA表达的影响.方法 将30只12周龄的雄性GK大鼠分为手术组(胃旁路术,10只)、假手术饮食控制组(10只)及假手术空白对照组(10只),术中取各组肝脏标本用于术后对照研究.观察手术前后空腹血糖、进食量及体质量变化情况.术后8周取各组肝脏标本,用实时荧光定量PCR(real-time PCR)检测各组肝脏手术前后PEPCK mRNA的表达变化.结果 术后8周,手术组空腹血糖由术前(17.6±2.1)mmol/L下降至(7.5±0.9)mmol/L;胃旁路术后PEPCK mRNA的相对表达量由术前的1.08±0.38下降至0.41±0.10(P＜0.01),与空白对照组(1.04±0.12)比较,差异具有统计学意义(P＜0.01);饮食控制组PEPCK mRNA的表达量则由术前的1.15±0.16上升至术后8周的2.54±0.82(P＜0.01);较手术组显著增高(P＜0.01).结论 胃旁路术降低2型糖尿病血糖的作用机制可能与降低PEPCK mRNA的表达有关.
目的 研究胃徬路術對2型糖尿病Goto-Kakizaki(GK)大鼠降糖作用及肝髒燐痠烯醇式丙酮痠羧激酶(PEPCK)mRNA錶達的影響.方法 將30隻12週齡的雄性GK大鼠分為手術組(胃徬路術,10隻)、假手術飲食控製組(10隻)及假手術空白對照組(10隻),術中取各組肝髒標本用于術後對照研究.觀察手術前後空腹血糖、進食量及體質量變化情況.術後8週取各組肝髒標本,用實時熒光定量PCR(real-time PCR)檢測各組肝髒手術前後PEPCK mRNA的錶達變化.結果 術後8週,手術組空腹血糖由術前(17.6±2.1)mmol/L下降至(7.5±0.9)mmol/L;胃徬路術後PEPCK mRNA的相對錶達量由術前的1.08±0.38下降至0.41±0.10(P＜0.01),與空白對照組(1.04±0.12)比較,差異具有統計學意義(P＜0.01);飲食控製組PEPCK mRNA的錶達量則由術前的1.15±0.16上升至術後8週的2.54±0.82(P＜0.01);較手術組顯著增高(P＜0.01).結論 胃徬路術降低2型糖尿病血糖的作用機製可能與降低PEPCK mRNA的錶達有關.
목적 연구위방로술대2형당뇨병Goto-Kakizaki(GK)대서강당작용급간장린산희순식병동산최격매(PEPCK)mRNA표체적영향.방법 장30지12주령적웅성GK대서분위수술조(위방로술,10지)、가수술음식공제조(10지)급가수술공백대조조(10지),술중취각조간장표본용우술후대조연구.관찰수술전후공복혈당、진식량급체질량변화정황.술후8주취각조간장표본,용실시형광정량PCR(real-time PCR)검측각조간장수술전후PEPCK mRNA적표체변화.결과 술후8주,수술조공복혈당유술전(17.6±2.1)mmol/L하강지(7.5±0.9)mmol/L;위방로술후PEPCK mRNA적상대표체량유술전적1.08±0.38하강지0.41±0.10(P＜0.01),여공백대조조(1.04±0.12)비교,차이구유통계학의의(P＜0.01);음식공제조PEPCK mRNA적표체량칙유술전적1.15±0.16상승지술후8주적2.54±0.82(P＜0.01);교수술조현저증고(P＜0.01).결론 위방로술강저2형당뇨병혈당적작용궤제가능여강저PEPCK mRNA적표체유관.
Objective To investigate the effects of gastric bypass surgery(GBP)on hepatic phosphoenolpyruvate carboxykinase(PEPCK)mRNA expression in type 2 diabetic Goto-Kakizaki rats.Methods Male GK rats were randomized into three groups:gastric bypass surgery(n=10),sham operation with diet restriction(n=10),and sham operation alone(n=10).Liver specimens of GK rats were collected during the intraoperative period for self-control study and 8 weeks after surgery.Fasting blood glucose,food intake,and body weight were recorded before surgery and 1,2,4,8 weeks after surgery.The expression of PEPCK mRNA was measured by real-time PCR.Results The fasting plasma glucose level decreased from(17.6±2.1)mmol/L before surgery to(7.5±0.9)mmol/L 8 weeks after surgery in GBP group.The level of PEPCK mRNA decreased from 1.08±0.38 before surgery to 0.41±0.10 8 weeks after surgery,significantly lower than that in sham operation alone group(1.04±0.12)(P＜0.01).The level of PEPCK mRNA in diet restriction group increased from 1.15±0.16 before surgery to 2.54±0.82 8 weeks after surgery(P＜0.01).The expression of PEPCK mRNA in diet restriction was significantly higher than that in CBP group(P＜0.01).Conclusions GBP can significantly improve hyperglycemia in type 2 diabetic GK rat models,which may be associated with the decrease of hepatic PEPCK mRNA level.
