CAJ | 학술논문

目的探讨结直肠癌组织中结缔组织生长因子(CTGF)mRNA和蛋白的表达及其与患者临床病理因素及5年生存率的关系.方法采用实时定量PCR技术检测92例结直肠癌及其相应远端正常结肠组织中CTGF mRNA的表达情况,再从中随机选取30对组织进行免疫组织化学检测CTGF蛋白表达,并对该组患者进行生存分析.结果 CTGF mRNA在结直肠癌组织中高表达,其CTGF蛋白阳性率73.3％(22/30)高于正常结肠组织的23.3％(7/30)(P＜0.01).合并淋巴结转移和肿瘤分期为Ⅲ、Ⅳ期者,CTGF mRNA表达水平和蛋白阳性率降低(均P＜0.05);CTGF蛋白阳性表达率低者浸润深度越深(P＜0.05).癌组织CTGF mRNA高表达组患者的5年生存率高于低表达组(68.3％比29.3％,P＜0.01).癌组织CTGF mRNA相对表达水平是患者生存期的独立影响因素(RR=2.960,95％ CI:1.491～5.877,P＜0.01),其预测患者预后的敏感性为64.9％,特异性为74.5％.结论 CTGF的异常表达与结直肠癌恶性生物学行为有关,CTGF低表达的结直肠癌患者预后不良.
목적 탐토결직장암조직중결체조직생장인자(CTGF)mRNA화단백적표체급기여환자림상병리인소급5년생존솔적관계.방법 채용실시정량PCR기술검측92례결직장암급기상응원단정상결장조직중CTGF mRNA적표체정황,재종중수궤선취30대조직진행면역조직화학검측CTGF단백표체,병대해조환자진행생존분석.결과 CTGF mRNA재결직장암조직중고표체,기CTGF단백양성솔73.3％(22/30)고우정상결장조직적23.3％(7/30)(P＜0.01).합병림파결전이화종류분기위Ⅲ、Ⅳ기자,CTGF mRNA표체수평화단백양성솔강저(균P＜0.05);CTGF단백양성표체솔저자침윤심도월심(P＜0.05).암조직CTGF mRNA고표체조환자적5년생존솔고우저표체조(68.3％비29.3％,P＜0.01).암조직CTGF mRNA상대표체수평시환자생존기적독립영향인소(RR=2.960,95％ CI:1.491～5.877,P＜0.01),기예측환자예후적민감성위64.9％,특이성위74.5％.결론 CTGF적이상표체여결직장암악성생물학행위유관,CTGF저표체적결직장암환자예후불량.
Objective To investigate the expression of connective tissue growth factor(CTGF)in colorectal cancer(CRC)and its association with clinicopathologic parameters and overall survival rate.Methods Fresh tumor tissues and matched distal normal colon tissues were collected from 92 patients diagnosed as CRC by surgical operation.The expression level of CTGF mRNA was quantified by quantitative reverse transcription PCR.Thirty out of 92 pairs of tissue specimens were selected randomly to detect CTGF protein by immunohistochemistry.All the cases were followed up to identify prognostic factors for survival.Results CTGF mRNA expression was up-regulated in CRC.The positive rate of CTGF protein expression tissues(73.3％)was significantly higher than that in the corresponding normal tissues(23.3％,P＜0.01).CTGF expression was lower in patients with lymphatic metastasis or stage Ⅲ/Ⅳdisease(all P＜0.05).A negative association was also observed between the CTGF protein positive rate and tumor infiltration depth(P＜0.05).The relative expression of CTGF mRNA in tumor tissues was classified into high and low expression groups.The 5-year cumulative survival rate was lower in patients with low CTGF expression(29.3％)as compared to those with high CTGF expressions(68.3％)(P＜0.01).Cox regression analysis revealed that the relative expression level of CTGF was independent factor of overall survival(RR=2.960,95％CI:1.491-1.587,P＜0.01).ROC curve analysis showed that sensitivity and specificity of CTGF mRNA expression for prediction of 5-year survival were 64.9％ and 74.5％,respectively.Conclusions The aberrant expression of CTGF is associated with the malignant biological behaviors of CRC.Low expression of CTGF is associated with worse prognosis of CRC.