中华胃肠外科杂志
中華胃腸外科雜誌
중화위장외과잡지
CHINESE JOURNAL OF GASTROINTESTINAL SURGERY
2012年
11期
1192-1196
,共5页
郑颖%杨成广%邱文才%常新
鄭穎%楊成廣%邱文纔%常新
정영%양성엄%구문재%상신
生长激素促分泌素%生长激素促分泌素受体%平滑肌肌条%小肠转运%肠神经丛
生長激素促分泌素%生長激素促分泌素受體%平滑肌肌條%小腸轉運%腸神經叢
생장격소촉분비소%생장격소촉분비소수체%평활기기조%소장전운%장신경총
Growth hormone secretagogue%Growth hormone secretagogue receptor%Smooth muscle strips%Small intestinal transit%Enteric plexus
目的 探讨生长激素促分泌素(ghrelin)对大鼠小肠平滑肌舒缩活动的影响及其机制.方法 制备迷走神经切断大鼠模型,腹腔注射不同浓度ghrelin(0、20、40和80 μg/kg),每种浓度6只大鼠,观察其对小肠转运的影响.体外实验观察在50 nmol/L卡巴胆碱(Cch)存在时,不同浓度的ghrelin(0.01、0.1、0.5、1.0 μmol/L)对肌条舒缩活动的影响,免疫荧光方法检测ghrelin受体(GHS-Rla)在小肠肌层中不同类型细胞的分布.结果 0、20、40和80μg/kg 4种浓度的ghrelin能够剂量依赖性地增加小肠转运率,分别为(25.4±1.0)％、(33.7±1.9)％、(39.3±2.4)％和(44.7±2.1)％,组间比较差异有统计学意义(P＜0.05).体外应用0.01、0.1、0.5和1.0 μmol/L 4种浓度的ghrelin能剂量依赖增强Cch引起的肌条收缩[(67.0±2.4)％、(149.5±3.3)％、(187.1±4.7)％、(213.5±3.4)％]和舒张[(35.3±1.1)％、(62.9±3.8)％、(79.6±2.7)％、(94.6±2.2)％]效应,组间比较差异均有统计学意义(均P＜0.05).免疫荧光检测显示,ghrelin受体主要分布在肠内肌层中的神经细胞膜上,平滑肌细胞膜上无分布.结论 ghrelin可通过直接作用于小肠肌层神经丛中的神经细胞而增强胆碱能神经递质引起的小肠平滑肌的舒缩活动.
目的 探討生長激素促分泌素(ghrelin)對大鼠小腸平滑肌舒縮活動的影響及其機製.方法 製備迷走神經切斷大鼠模型,腹腔註射不同濃度ghrelin(0、20、40和80 μg/kg),每種濃度6隻大鼠,觀察其對小腸轉運的影響.體外實驗觀察在50 nmol/L卡巴膽堿(Cch)存在時,不同濃度的ghrelin(0.01、0.1、0.5、1.0 μmol/L)對肌條舒縮活動的影響,免疫熒光方法檢測ghrelin受體(GHS-Rla)在小腸肌層中不同類型細胞的分佈.結果 0、20、40和80μg/kg 4種濃度的ghrelin能夠劑量依賴性地增加小腸轉運率,分彆為(25.4±1.0)％、(33.7±1.9)％、(39.3±2.4)％和(44.7±2.1)％,組間比較差異有統計學意義(P＜0.05).體外應用0.01、0.1、0.5和1.0 μmol/L 4種濃度的ghrelin能劑量依賴增彊Cch引起的肌條收縮[(67.0±2.4)％、(149.5±3.3)％、(187.1±4.7)％、(213.5±3.4)％]和舒張[(35.3±1.1)％、(62.9±3.8)％、(79.6±2.7)％、(94.6±2.2)％]效應,組間比較差異均有統計學意義(均P＜0.05).免疫熒光檢測顯示,ghrelin受體主要分佈在腸內肌層中的神經細胞膜上,平滑肌細胞膜上無分佈.結論 ghrelin可通過直接作用于小腸肌層神經叢中的神經細胞而增彊膽堿能神經遞質引起的小腸平滑肌的舒縮活動.
목적 탐토생장격소촉분비소(ghrelin)대대서소장평활기서축활동적영향급기궤제.방법 제비미주신경절단대서모형,복강주사불동농도ghrelin(0、20、40화80 μg/kg),매충농도6지대서,관찰기대소장전운적영향.체외실험관찰재50 nmol/L잡파담감(Cch)존재시,불동농도적ghrelin(0.01、0.1、0.5、1.0 μmol/L)대기조서축활동적영향,면역형광방법검측ghrelin수체(GHS-Rla)재소장기층중불동류형세포적분포.결과 0、20、40화80μg/kg 4충농도적ghrelin능구제량의뢰성지증가소장전운솔,분별위(25.4±1.0)％、(33.7±1.9)％、(39.3±2.4)％화(44.7±2.1)％,조간비교차이유통계학의의(P＜0.05).체외응용0.01、0.1、0.5화1.0 μmol/L 4충농도적ghrelin능제량의뢰증강Cch인기적기조수축[(67.0±2.4)％、(149.5±3.3)％、(187.1±4.7)％、(213.5±3.4)％]화서장[(35.3±1.1)％、(62.9±3.8)％、(79.6±2.7)％、(94.6±2.2)％]효응,조간비교차이균유통계학의의(균P＜0.05).면역형광검측현시,ghrelin수체주요분포재장내기층중적신경세포막상,평활기세포막상무분포.결론 ghrelin가통과직접작용우소장기층신경총중적신경세포이증강담감능신경체질인기적소장평활기적서축활동.
Objective To investigate the effect of growth hormone secretagogue(ghrelin)on the contraction and relaxation of small intestinal smooth muscle in rats and its mechanism.Methods Twenty-four vagotomized rats were injected intraperitoneally with different concentrations of ghrelin(0,20,40,80 μg/kg).The small intestinal transit were observed.The effect of ghrelin(0.01,0.1,0.5,1.0 μmol/L)on the contraction and relaxation of rat small intestinal smooth muscle strips was observed in vitro in the presence of carbachol(50 nmol/L),the locations of ghrelin receptors(GHS-R1a)on different cells in small intestinal muscle layers were detected by immunofluorescence.Results With the increase of concentrations,ghrelin elevated the percentage of small intestinal transit[(25.4±1.0)％,(33.7±1.9)％,(39.3±2.4)％,(44.7±2.1)％]in a dose-dependent manner,and the differences were statistically significant among groups(P＜0.05).Ghrelin could also enhance the contraction[(67.0±2.4)％,(149.5±3.3)％,(187.1±4.7)％,(213.5±3.4)％]and relaxation[(35.3±1.1)％,(62.9±3.8)％,(79.6±2.7)％,(94.6±2.2)％]of smooth muscle strips mediated by Cch in a dose-dependent manner,and the differences were statistically significant among groups(P＜0.05).Immunofluorescence revealed that ghrelin receptors mainly located on membrane of the nerve cells in the muscle layers,while no receptors were observed on membrane of the smooth muscle cells.Conclusion Ghrelin may enhance the effect of the contraction and relaxation of the rat small intestinal smooth muscle mediated by cholinergic neurotransmitters by activating the nerve cells in the enteric plexus.
