中华胃肠外科杂志
中華胃腸外科雜誌
중화위장외과잡지
CHINESE JOURNAL OF GASTROINTESTINAL SURGERY
2013年
1期
75-79
,共5页
裴海平%李小龙%朱红%曾亮%黄林生
裴海平%李小龍%硃紅%曾亮%黃林生
배해평%리소룡%주홍%증량%황림생
左半结肠肿瘤%右半结肠肿瘤%葡萄糖调节蛋白78 kD%热休克蛋白20%蛋白质组学
左半結腸腫瘤%右半結腸腫瘤%葡萄糖調節蛋白78 kD%熱休剋蛋白20%蛋白質組學
좌반결장종류%우반결장종류%포도당조절단백78 kD%열휴극단백20%단백질조학
Left-sided colonic neoplasms%Right-sided colonic neoplasms%Glucose regulated protein 78 kD%Heat shock protein 20%Proteomics
目的 分析左半结肠癌与右半结肠癌肿瘤组织中差异蛋白表达情况.方法 收集人左半结肠癌和右半结肠癌组织标本各7例,提取组织蛋白并进行二维凝胶电泳、质谱分析及生物信息学分离,鉴定二者中差异表达蛋白质.应用免疫组织化学SP法检测葡萄糖调节蛋白78 kD(GRP78)和热休克蛋白20(HSP20)在50例左半和50例右半结肠癌组织中的表达情况.结果 筛选并成功鉴定出左半和右半结肠癌中16种差异表达蛋白质;与右半结肠癌比较,左半结肠癌10种蛋白表达上调,6种蛋白表达下调,其中GRP78在左半结肠癌中表达上调,HSP20在左半结肠癌中表达下调.免疫组织化学检测示,GRP78在左半和右半结肠癌组织的阳性表达率分别为78％(39/50)和56％(28/50),差异有统计学意义(P＜0.05);HSP20在左半和右半结肠癌的阳性表达率分别为34％(17/50)和72％(36/50),差异亦有统计学意义(P＜0.05).GRP78表达与肿瘤分化程度、浸润层次、TNM分期、有无淋巴结转移以及有无肝转移有关(P＜0.05),而HSP20的表达与肿瘤大体形态、TNM分期以及有无淋巴结转移有关(P＜0.05).结论 左半结肠癌和右半结肠癌的蛋白质组存在差异,这些可能是左半与右半结肠癌生物学行为存在差异的原因.
目的 分析左半結腸癌與右半結腸癌腫瘤組織中差異蛋白錶達情況.方法 收集人左半結腸癌和右半結腸癌組織標本各7例,提取組織蛋白併進行二維凝膠電泳、質譜分析及生物信息學分離,鑒定二者中差異錶達蛋白質.應用免疫組織化學SP法檢測葡萄糖調節蛋白78 kD(GRP78)和熱休剋蛋白20(HSP20)在50例左半和50例右半結腸癌組織中的錶達情況.結果 篩選併成功鑒定齣左半和右半結腸癌中16種差異錶達蛋白質;與右半結腸癌比較,左半結腸癌10種蛋白錶達上調,6種蛋白錶達下調,其中GRP78在左半結腸癌中錶達上調,HSP20在左半結腸癌中錶達下調.免疫組織化學檢測示,GRP78在左半和右半結腸癌組織的暘性錶達率分彆為78％(39/50)和56％(28/50),差異有統計學意義(P＜0.05);HSP20在左半和右半結腸癌的暘性錶達率分彆為34％(17/50)和72％(36/50),差異亦有統計學意義(P＜0.05).GRP78錶達與腫瘤分化程度、浸潤層次、TNM分期、有無淋巴結轉移以及有無肝轉移有關(P＜0.05),而HSP20的錶達與腫瘤大體形態、TNM分期以及有無淋巴結轉移有關(P＜0.05).結論 左半結腸癌和右半結腸癌的蛋白質組存在差異,這些可能是左半與右半結腸癌生物學行為存在差異的原因.
목적 분석좌반결장암여우반결장암종류조직중차이단백표체정황.방법 수집인좌반결장암화우반결장암조직표본각7례,제취조직단백병진행이유응효전영、질보분석급생물신식학분리,감정이자중차이표체단백질.응용면역조직화학SP법검측포도당조절단백78 kD(GRP78)화열휴극단백20(HSP20)재50례좌반화50례우반결장암조직중적표체정황.결과 사선병성공감정출좌반화우반결장암중16충차이표체단백질;여우반결장암비교,좌반결장암10충단백표체상조,6충단백표체하조,기중GRP78재좌반결장암중표체상조,HSP20재좌반결장암중표체하조.면역조직화학검측시,GRP78재좌반화우반결장암조직적양성표체솔분별위78％(39/50)화56％(28/50),차이유통계학의의(P＜0.05);HSP20재좌반화우반결장암적양성표체솔분별위34％(17/50)화72％(36/50),차이역유통계학의의(P＜0.05).GRP78표체여종류분화정도、침윤층차、TNM분기、유무림파결전이이급유무간전이유관(P＜0.05),이HSP20적표체여종류대체형태、TNM분기이급유무림파결전이유관(P＜0.05).결론 좌반결장암화우반결장암적단백질조존재차이,저사가능시좌반여우반결장암생물학행위존재차이적원인.
Objective To analyze the differential protein expression of left-sided colon cancer and right-sided colon cancer.Methods Tissue samples of left-sided colon cancer(n=7) and right-sided colon cancer(n=7) were collected.Tissue protein was abstracted and two-dimensional gel electrophoresis (2-DE) was used to examine the gel images.Peptide mass fingerprintings (PMF) was acquired by matrix assisted laser desorption/ionization time-of-flight mass spectrometry(MALDI-TOF-MS) and the proteins were identified by data searching with bioinformatics.Immunohistochemical SP method was used for the detection of glucose regulated protein 78 kD (GRP78) and heat shock protein 20 (HSP20) in left-sided colon cancer (n =50) and right-sided colon cancer (n =50) tissues.Results Sixteen differentiating protein spots were identified.Compared with right-sided colon cancer,10 proteins including GRP78 up-regulated and 6 proteins including HSP20 down-regulated in left-sided colon cancer.Immunohistochemical detection showed that in left and right sided colon cancer,the positive expression rate of GRP78 was 78％(39/50) and 56％(28/50) and the positive expression rate of HSP20 was 34％ (17/50) and 72％ (36/50),respectively,and the differences were statistically significant (both P＜0.05).The positive rate of GRP78 was associated with tumor differentiation,infiltration layer,TNM staging,lymph node metastasis,and liver metastasis,while the positive rate of HSP20 was associated with tumor gross morphology,TNM staging,and lymph node metastasis (P＜0.05).Conclusions There are differentially expressed proteins between left-sided colon cancer and right-sided colon cancer,especially for GRP78 and HSP20,which may be the cause leading to the biological differences between left-sided and right-sided colon cancer.
