CAJ | 학술논문

目的观察氟尿嘧啶(5-FU)对人结肠癌SW480细胞ABCG2表达的影响.方法用不同药物浓度的5-FU处理SW480细胞,用CCK8法检测5-FU在SW480中的IC50,流式细胞仪检测SW480细胞ABCG2的阳性表达率,RT-PCR检测ABCG2的mRNA在SW480细胞中的表达差异.结果 5-FU对SW480细胞的IC50随着药物浓度的增加而升高(P＜0.05).流式细胞仪检测发现,正常SW480细胞(A组)中ABCG2阳性表达率为(6.26±0.86)％;在药物处理48 h后即刻检测时(B组)的阳性表达率下降至(3.43±1.18)％(P＜0.05);在药物处理48 h后的第2代细胞检测时(C组)则升高至(12.91±3.42)％(P＜0.05).3组ABCG2 mRNA表达趋势与流式细胞仪检测结果的趋势一致.结论不同浓度的5-FU可以影响人结肠癌SW480细胞ABCG2的表达.
목적 관찰불뇨밀정(5-FU)대인결장암SW480세포ABCG2표체적영향.방법 용불동약물농도적5-FU처리SW480세포,용CCK8법검측5-FU재SW480중적IC50,류식세포의검측SW480세포ABCG2적양성표체솔,RT-PCR검측ABCG2적mRNA재SW480세포중적표체차이.결과 5-FU대SW480세포적IC50수착약물농도적증가이승고(P＜0.05).류식세포의검측발현,정상SW480세포(A조)중ABCG2양성표체솔위(6.26±0.86)％;재약물처리48 h후즉각검측시(B조)적양성표체솔하강지(3.43±1.18)％(P＜0.05);재약물처리48 h후적제2대세포검측시(C조)칙승고지(12.91±3.42)％(P＜0.05).3조ABCG2 mRNA표체추세여류식세포의검측결과적추세일치.결론 불동농도적5-FU가이영향인결장암SW480세포ABCG2적표체.
Objective To investigate the effect of 5-fluorouracil (5-FU) on the expression of ATP-binding cassette superfamily G member 2(ABCG2) in human colon cancer cell SW480.Methods SW480 cells were treated with various concentrations of 5-FU.CCK8 assay was utilized to detect the 5-FU IC50 to SW480 cells.Positive expression of ABCG2 was detected by flow cytometry,and mRNA expression of ABCG2 was detected by real time polymerase chain reaction (RT-PCR).Results The 5-FU IC50 to SW480 cells increased as the drug concentration increased(P＜0.05).Flow cytometry revealed that positive expression rate of ABCG2 in normal SW480 cells (group A) was (6.26±0.86)％.Immediately after treatment with 5-FU for 48 hours,the positive expression rate of ABCG2 (group B) was (3.43±1.18)％(P＜0.05).In the second passage of cells after treatment with 5-FU for 48 hours,the positive expression rate of ABCG2 (group C) was (12.91±3.42)％(P＜0.05).The mRNA expression of ABCG2 detected by RT-PCR was in accordance with the results from flow cytometry.Conclusion Expression of ABCG2 in SW480 cells can be affected by various concentrations of 5-FU.