中华胃肠外科杂志
中華胃腸外科雜誌
중화위장외과잡지
CHINESE JOURNAL OF GASTROINTESTINAL SURGERY
2013年
2期
183-187
,共5页
姚海波%徐园%陈乐高%关天培%马英玉%陶厚权%邵钦树
姚海波%徐園%陳樂高%關天培%馬英玉%陶厚權%邵欽樹
요해파%서완%진악고%관천배%마영옥%도후권%소흠수
胃肿瘤%醛酮还原酶%预后
胃腫瘤%醛酮還原酶%預後
위종류%철동환원매%예후
Stomach neoplasms%Aldo-keto reductase%Prognosis
目的 探讨醛酮还原酶1-B10在胃癌组织中的表达及其与临床病理特征和预后的关系.方法 通过荧光定量PCR法检测36例胃癌组织及其配对癌旁组织中AKR1B10 mRNA的表达量,采用免疫组织化学法检测100例原发胃癌组织及70例非肿瘤胃黏膜组织中AKR1B10蛋白表达情况.结果 PCR结果显示,91.7％(33/36)癌旁非肿瘤胃黏膜组织中AKR1B10 mRNA的表达高于其配对胃癌组织[8.3％(3/36),P=0.000].100例胃癌组织中33例(33.0％)AKR1B10蛋白阳性表达,70例非肿瘤胃黏膜上皮中65例(92.9％)AKR1B10的阳性表达,两者比较,差异有统计学意义(P=0.000).胃癌组织中AKR1B10的表达与患者性别、年龄、肿瘤部位及分化程度无关(P＞0.05),而与肿瘤大小(P=0.000)、浸润深度(P=0.004)、淋巴结转移(P=0.028)、远处转移(P=0.031)和临床TNM分期(P=0.000)有关.AKR1B10阳性表达患者的5年生存率显著高于阴性表达者(60.6％比32.8％,P＜0.01).结论 胃癌组织中AKR1B10的表达下调与胃癌进展有关,提示预后不良.
目的 探討醛酮還原酶1-B10在胃癌組織中的錶達及其與臨床病理特徵和預後的關繫.方法 通過熒光定量PCR法檢測36例胃癌組織及其配對癌徬組織中AKR1B10 mRNA的錶達量,採用免疫組織化學法檢測100例原髮胃癌組織及70例非腫瘤胃黏膜組織中AKR1B10蛋白錶達情況.結果 PCR結果顯示,91.7％(33/36)癌徬非腫瘤胃黏膜組織中AKR1B10 mRNA的錶達高于其配對胃癌組織[8.3％(3/36),P=0.000].100例胃癌組織中33例(33.0％)AKR1B10蛋白暘性錶達,70例非腫瘤胃黏膜上皮中65例(92.9％)AKR1B10的暘性錶達,兩者比較,差異有統計學意義(P=0.000).胃癌組織中AKR1B10的錶達與患者性彆、年齡、腫瘤部位及分化程度無關(P＞0.05),而與腫瘤大小(P=0.000)、浸潤深度(P=0.004)、淋巴結轉移(P=0.028)、遠處轉移(P=0.031)和臨床TNM分期(P=0.000)有關.AKR1B10暘性錶達患者的5年生存率顯著高于陰性錶達者(60.6％比32.8％,P＜0.01).結論 胃癌組織中AKR1B10的錶達下調與胃癌進展有關,提示預後不良.
목적 탐토철동환원매1-B10재위암조직중적표체급기여림상병리특정화예후적관계.방법 통과형광정량PCR법검측36례위암조직급기배대암방조직중AKR1B10 mRNA적표체량,채용면역조직화학법검측100례원발위암조직급70례비종류위점막조직중AKR1B10단백표체정황.결과 PCR결과현시,91.7％(33/36)암방비종류위점막조직중AKR1B10 mRNA적표체고우기배대위암조직[8.3％(3/36),P=0.000].100례위암조직중33례(33.0％)AKR1B10단백양성표체,70례비종류위점막상피중65례(92.9％)AKR1B10적양성표체,량자비교,차이유통계학의의(P=0.000).위암조직중AKR1B10적표체여환자성별、년령、종류부위급분화정도무관(P＞0.05),이여종류대소(P=0.000)、침윤심도(P=0.004)、림파결전이(P=0.028)、원처전이(P=0.031)화림상TNM분기(P=0.000)유관.AKR1B10양성표체환자적5년생존솔현저고우음성표체자(60.6％비32.8％,P＜0.01).결론 위암조직중AKR1B10적표체하조여위암진전유관,제시예후불량.
Objectitv To investigate the association of AKR1B10 expression in gastric cancer tissues with clinicopathologic features and prognosis of gastric cancer patients.Methods Real-time polymerase chain reaction (RT-PCR) was conducted to detect AKR1B10 mRNA expression in gastric cancer and adjacent gastric mucosa tissues (n=36).AKR1B10 protein expression was measured by immunohistochemistry in primary gastric cancer tissues(n=100) and non-tumorous gastric mucosa tissues (n=70).Results RT-PCR results confirmed that AKR1B10 was significantly down-regulated in gastric cancer tissues compared with that in paired adjacent mucosa[8.3％(3/36) vs.91.7％(33/36),P=0.000].Immunohistochemistry revealed that the percentage of AKR1B10 positive specimens in gastric carcinoma was lower than that in normal specimens[33.0％(33/100) vs.92.9％(65/70),P=0.000].The frequencies of positive AKR1B10 in patients was significantly correlated with tumor size (P=0.000),invasive depth (P=0.004),lymph node metastasis(P=0.028),distant metastasis(P=0.031) and TNM stages(P=0.000).The 5-year survival rate of positive AKR1B10 group was significantly higher as compared to negative group(60.6％ vs.32.8％,P＜0.01).Conclusion The down-regulation of AKR1B10 expression in gastric cancer may be associated with the progress of gastric cancer is suggestive of poor prognosis.
