中华胃肠外科杂志
中華胃腸外科雜誌
중화위장외과잡지
CHINESE JOURNAL OF GASTROINTESTINAL SURGERY
2013年
5期
484-488
,共5页
刘如璐%赵奎%孙家磊%郁立衍%朱宝松%杨晓东%邢春根
劉如璐%趙奎%孫傢磊%鬱立衍%硃寶鬆%楊曉東%邢春根
류여로%조규%손가뢰%욱립연%주보송%양효동%형춘근
胃肿瘤%磷脂酰肌醇-3激酶%腺病毒
胃腫瘤%燐脂酰肌醇-3激酶%腺病毒
위종류%린지선기순-3격매%선병독
Stomach neoplasms%Phosphatidylinositol-3-kinases%Recombinant adenovirus
目的 研究利用重组腺病毒特异性阻断Ⅰ型磷脂酰肌醇-3激酶(PI3K)信号转导通路,对裸鼠胃癌移植瘤的影响,并探讨其相关作用机制.方法 用人胃癌细胞SGC7901建立裸鼠胃癌移植瘤模型,分为3组,分别给予重组腺病毒PI3K(Ⅰ)-RNAi-AD、空病毒NC-RNAi-GFP-AD和生理盐水处理.给药后第3、6和9天分别取5只裸鼠处死,测量移植瘤体积,计算抑瘤率；应用免疫组织化学法检测移植瘤组织的TNF-α、环氧化酶2(COX2)、P53、增殖细胞核抗原(PCNA)、E-cadherin及nm23/DNPK的表达水平.结果 给药后第3、6和9天,PI3K(Ⅰ)-RNAi-AD组的抑瘤率分别为14.2％,21.0％和28.1％,显著高于空病毒组(1.3％、1.9％和2.0％,均P＜0.05).给药后第9天,PI3K(Ⅰ)-RNAi-AD组TNF-α、P53、E-cadherin、nm23/DNPK蛋白的表达均明显高于空病毒组和对照组；而COX2、PCNA的表达则明显减少(P＜0.05).结论 重组腺病毒PI3K(Ⅰ)-RNAi-AD对裸鼠胃癌移植瘤有一定的抗肿瘤作用,其机制为通过抑制胃癌细胞增殖、血管生成、降低侵袭能力等多个途径共同发挥抑癌效应.
目的 研究利用重組腺病毒特異性阻斷Ⅰ型燐脂酰肌醇-3激酶(PI3K)信號轉導通路,對裸鼠胃癌移植瘤的影響,併探討其相關作用機製.方法 用人胃癌細胞SGC7901建立裸鼠胃癌移植瘤模型,分為3組,分彆給予重組腺病毒PI3K(Ⅰ)-RNAi-AD、空病毒NC-RNAi-GFP-AD和生理鹽水處理.給藥後第3、6和9天分彆取5隻裸鼠處死,測量移植瘤體積,計算抑瘤率；應用免疫組織化學法檢測移植瘤組織的TNF-α、環氧化酶2(COX2)、P53、增殖細胞覈抗原(PCNA)、E-cadherin及nm23/DNPK的錶達水平.結果 給藥後第3、6和9天,PI3K(Ⅰ)-RNAi-AD組的抑瘤率分彆為14.2％,21.0％和28.1％,顯著高于空病毒組(1.3％、1.9％和2.0％,均P＜0.05).給藥後第9天,PI3K(Ⅰ)-RNAi-AD組TNF-α、P53、E-cadherin、nm23/DNPK蛋白的錶達均明顯高于空病毒組和對照組；而COX2、PCNA的錶達則明顯減少(P＜0.05).結論 重組腺病毒PI3K(Ⅰ)-RNAi-AD對裸鼠胃癌移植瘤有一定的抗腫瘤作用,其機製為通過抑製胃癌細胞增殖、血管生成、降低侵襲能力等多箇途徑共同髮揮抑癌效應.
목적 연구이용중조선병독특이성조단Ⅰ형린지선기순-3격매(PI3K)신호전도통로,대라서위암이식류적영향,병탐토기상관작용궤제.방법 용인위암세포SGC7901건립라서위암이식류모형,분위3조,분별급여중조선병독PI3K(Ⅰ)-RNAi-AD、공병독NC-RNAi-GFP-AD화생리염수처리.급약후제3、6화9천분별취5지라서처사,측량이식류체적,계산억류솔；응용면역조직화학법검측이식류조직적TNF-α、배양화매2(COX2)、P53、증식세포핵항원(PCNA)、E-cadherin급nm23/DNPK적표체수평.결과 급약후제3、6화9천,PI3K(Ⅰ)-RNAi-AD조적억류솔분별위14.2％,21.0％화28.1％,현저고우공병독조(1.3％、1.9％화2.0％,균P＜0.05).급약후제9천,PI3K(Ⅰ)-RNAi-AD조TNF-α、P53、E-cadherin、nm23/DNPK단백적표체균명현고우공병독조화대조조；이COX2、PCNA적표체칙명현감소(P＜0.05).결론 중조선병독PI3K(Ⅰ)-RNAi-AD대라서위암이식류유일정적항종류작용,기궤제위통과억제위암세포증식、혈관생성、강저침습능력등다개도경공동발휘억암효응.
Objective To investigate the effect of recombinant adenovirus (phosphatidylinositol3-kinases(PI3K)(Ⅰ)-RNAi-AD which blocks the class Ⅰ PI3K signaling pathway on gastric carcinoma cells xenografts in nude mice.Methods Subcutaneous tumor models of nude mice were established with SGC7901 cells and randomly divided into PI3K(Ⅰ)-RNAi-AD group,NC-RNAi-GFP-AD group and control group.The tumor size and the inhibitory rate of tumor growth on days 3,6,and 9 after cell transplantation were measured.The expression of TNF-α,COX2,P53,PCNA,E-cadherin and nm23/DNPK in tumor tissues were detected by immunohistochemistry.Results Tumor growth was significantly inhibited in the PI3K(Ⅰ)-RNAi-AD group(14.2％,21.0％,and 28.1％) on days 3,6,9 compared with NC-RNAi-GFP-AD group(1.3％,1.9％,and 2.0％,all P＜0.05).The expressions of TNF-α,P53,E-cadherin and nm23/DNPK were up-regulated,and the expressions of COX2 and PCNA were down-regulated in the PI3K(Ⅰ)-RNAi-AD group by immunohistochemical staining(all P＜0.05).Conclusions PI3K(Ⅰ)-RNAi-AD can inhibit the growth of SGC7901 cell transplantation tumor in vivo in nude mice by inhibiting cell growth,reducing the capacity of tumor invasion and inhibiting tumor angiogenesis.
