CAJ | 학술논문

目的探讨穿心莲内酯(andrographolide,AD)对人胃癌细胞株BGC-823细胞增殖、细胞周期以及细胞凋亡的影响.方法分别采用M'TT法、流式细胞术和流式细胞仪AnnexinV/PI双染色法检测AD对BGC-823细胞体外增殖、细胞周期和细胞凋亡的影响；应用光镜和透射电镜观察不同浓度的AD作用后BGC-823细胞形态学改变.结果各浓度组AD均对人低分化胃癌细胞株BGC-823的增殖有抑制作用,并具有时间和浓度依赖关系(均P＜0.05).浓度7.5 μg/ml以下的AD抑制效果较弱,而15.0～60.0 μg/ml抑制效果显著提高(P＜0.05),60.0 μg/ml以上抑制率增高不显著(P＞0.05).24、48和72 h的IC50分别为(35.3±4.3)、(25.5±3.5)和(18.2±2.7) μg/ml.BGC-823细胞经AD作用后,G0/G1期细胞的比例增加,S期和G2/M期细胞的比例下降,细胞被阻滞在G0/G1期,呈浓度依赖关系.AD浓度为7.5、10.0和15.0 μg/ml组作用24 h后,早期凋亡率分别为(19.3±4.7)％、(29.4±4.1)％和(52.7±6.7)％,晚期凋亡率为(10.8±1.8)％、(10.9±4.7)％和(14.7±4.8)％,均显著高于阴性对照组的早期凋亡率[(3.4±1.0)％]和晚期凋亡率[(4.1±0.7)％],差异有统计学意义(均P＜0.05),并呈浓度依赖关系.结论 AD能抑制BGC-823细胞增殖、阻滞其细胞周期在G0/G1期和诱导其细胞凋亡,是潜在的胃癌抗肿瘤中药制剂成分.
목적 탐토천심련내지(andrographolide,AD)대인위암세포주BGC-823세포증식、세포주기이급세포조망적영향.방법 분별채용M'TT법、류식세포술화류식세포의AnnexinV/PI쌍염색법검측AD대BGC-823세포체외증식、세포주기화세포조망적영향；응용광경화투사전경관찰불동농도적AD작용후BGC-823세포형태학개변.결과 각농도조AD균대인저분화위암세포주BGC-823적증식유억제작용,병구유시간화농도의뢰관계(균P＜0.05).농도7.5 μg/ml이하적AD억제효과교약,이15.0～60.0 μg/ml억제효과현저제고(P＜0.05),60.0 μg/ml이상억제솔증고불현저(P＞0.05).24、48화72 h적IC50분별위(35.3±4.3)、(25.5±3.5)화(18.2±2.7) μg/ml.BGC-823세포경AD작용후,G0/G1기세포적비례증가,S기화G2/M기세포적비례하강,세포피조체재G0/G1기,정농도의뢰관계.AD농도위7.5、10.0화15.0 μg/ml조작용24 h후,조기조망솔분별위(19.3±4.7)％、(29.4±4.1)％화(52.7±6.7)％,만기조망솔위(10.8±1.8)％、(10.9±4.7)％화(14.7±4.8)％,균현저고우음성대조조적조기조망솔[(3.4±1.0)％]화만기조망솔[(4.1±0.7)％],차이유통계학의의(균P＜0.05),병정농도의뢰관계.결론 AD능억제BGC-823세포증식、조체기세포주기재G0/G1기화유도기세포조망,시잠재적위암항종류중약제제성분.
Objective To investigate the effect of andrographolide (AD) on proliferation,cell cycle and apoptosis of human gastric cells line BGC-823.Methods MTT assay,flow cytometry and Annexin-V/PI double-staining flow cytometry assay were used to evaluate the effect of AD on proliferation,cell cycle and apoptosis of BGC-823 cells respectively.Optical microscope and transmission electron microscopy were used to observe the cell morphological changes.Results A time-and concentration-dependent proliferative inhibition effect of AD was demonstrated in BGC-823 cells.AD concentration lower than 7.5 mg/L possessed weak inhibitory effect,while concentration between 15.0-60.0 mg/L possessed higher inhibitory effect.The concentration higher than 60.0 mg/L had no significant increase of inhibitory effect.IC50 of AD at 24,48 and 72 h was (35.3±4.3),(25.5±3.5) and (18.2±2.7) mg/L respectively.Compared with the negative control group,the number of G0/G1 phase cells increased significantly (P＜0.05),while the number of S and G2/M phase cells decreased after incubation with AD for 48 h,and the alteration was in a concentration-dependent manner.AD arrested BGC-823 cells at the G0/G1 phase of the cell cycle.After incubation with 7.5,10.0 and 15.0 mg/L AD for 24 h,the early apoptotic rates of BGC-823 cells were(19.3±4.7)％,(29.4±4.1)％ and(52.7±6.7)％ respectively,and the late apoptotic rates were (10.8±1.8)％,(10.9±4.7)％ and (14.7±4.8)％ respectively.Both the early apoptotie rates and the late apoptotic rates increased significantly compared to the control group (all P＜0.05),and the alteration was in a concentration-dependent manner.Conclusion Andrographolide can inhibit BGC-823 cells proliferation,arrest BGC-823 cells in G0/G1 phase and induce apoptosis,and may be a potential traditional Chinese medicine with anti-cancer effect.