中华胃肠外科杂志
中華胃腸外科雜誌
중화위장외과잡지
CHINESE JOURNAL OF GASTROINTESTINAL SURGERY
2013年
9期
874-877
,共4页
郑庆丰%柳硕岩%王海燕%王枫%王镇%陈啸风%王健键%应敏刚%郑雄伟
鄭慶豐%柳碩巖%王海燕%王楓%王鎮%陳嘯風%王健鍵%應敏剛%鄭雄偉
정경봉%류석암%왕해연%왕풍%왕진%진소풍%왕건건%응민강%정웅위
食管肿瘤%畸胎瘤源性生长因子%RNA干扰%生物学特性
食管腫瘤%畸胎瘤源性生長因子%RNA榦擾%生物學特性
식관종류%기태류원성생장인자%RNA간우%생물학특성
Esophageal neoplasmas%PC cell-derived growth factor%RNA interference%Biological characteristics
目的 探讨RNA干扰畸胎瘤源性生长因子(PCDGF)基因对食管鳞癌细胞Eca-109的影响.方法 脂质体介导PCDGF-shRNA的表达载体转染Eca-109细胞,应用RT-PCR、Westernblot检测转染后细胞PCDGF mRNA及蛋白的表达情况,分别采用Counting Kit-8(CCK-8)试剂盒和Boyden小室法检测转染后Eca-109细胞的增殖能力和侵袭能力.结果 转染组PCDGF mRNA和蛋白表达水平均较其他组下调.转染24、48和72 h转染组细胞增殖均受到抑制,抑制率分别为20.4％、21.1％和20.9％,其细胞增殖活性较未转染组、阴性质粒组和脂质体组均明显降低(均P＜0.05).未转染组、阴性质粒组、脂质体组和转染组的细胞迁移数分别为118.8±12.0、100.8±9.0、114.3±4.7和53.5±16.3,转染组与其余3组比较,差异均有统计学意义(P=0.001,P=0.002,P=0.002).结论 RNA干扰PCDGF基因可抑制食管鳞癌细胞的体外增殖及侵袭能力,PCDGF基因可能成为基因治疗的新靶点.
目的 探討RNA榦擾畸胎瘤源性生長因子(PCDGF)基因對食管鱗癌細胞Eca-109的影響.方法 脂質體介導PCDGF-shRNA的錶達載體轉染Eca-109細胞,應用RT-PCR、Westernblot檢測轉染後細胞PCDGF mRNA及蛋白的錶達情況,分彆採用Counting Kit-8(CCK-8)試劑盒和Boyden小室法檢測轉染後Eca-109細胞的增殖能力和侵襲能力.結果 轉染組PCDGF mRNA和蛋白錶達水平均較其他組下調.轉染24、48和72 h轉染組細胞增殖均受到抑製,抑製率分彆為20.4％、21.1％和20.9％,其細胞增殖活性較未轉染組、陰性質粒組和脂質體組均明顯降低(均P＜0.05).未轉染組、陰性質粒組、脂質體組和轉染組的細胞遷移數分彆為118.8±12.0、100.8±9.0、114.3±4.7和53.5±16.3,轉染組與其餘3組比較,差異均有統計學意義(P=0.001,P=0.002,P=0.002).結論 RNA榦擾PCDGF基因可抑製食管鱗癌細胞的體外增殖及侵襲能力,PCDGF基因可能成為基因治療的新靶點.
목적 탐토RNA간우기태류원성생장인자(PCDGF)기인대식관린암세포Eca-109적영향.방법 지질체개도PCDGF-shRNA적표체재체전염Eca-109세포,응용RT-PCR、Westernblot검측전염후세포PCDGF mRNA급단백적표체정황,분별채용Counting Kit-8(CCK-8)시제합화Boyden소실법검측전염후Eca-109세포적증식능력화침습능력.결과 전염조PCDGF mRNA화단백표체수평균교기타조하조.전염24、48화72 h전염조세포증식균수도억제,억제솔분별위20.4％、21.1％화20.9％,기세포증식활성교미전염조、음성질립조화지질체조균명현강저(균P＜0.05).미전염조、음성질립조、지질체조화전염조적세포천이수분별위118.8±12.0、100.8±9.0、114.3±4.7화53.5±16.3,전염조여기여3조비교,차이균유통계학의의(P=0.001,P=0.002,P=0.002).결론 RNA간우PCDGF기인가억제식관린암세포적체외증식급침습능력,PCDGF기인가능성위기인치료적신파점.
Objective To investigate the effect of PC cell-derived growth factor (PCDGF) RNA interference on esophageal squamous carcinoma cells Eca-109 in vitro.Methods The PCDGF-shRNA expression vector was transfected into the Eca-109 cells by liposome.After transfection,the mRNA and protein expressions of PCDGF were detected by RT-PCR and Western-blot respectively.Cell Counting Kit-8 (CCK-8) assay and Boyden chamber method were performed to measure the cell proliferation and invasion ability respectively.Results The expression levels of PCDGF mRNA and protein were both decreased in Eca-109 cells transfected with PCDGF-shRNA expression vector (transfection group).Twenty-four,48 and 72 h after transfection,the cells proliferation in the transfection group was inhibited,and the inhibition rate was 20.4％,21.1％ and 20.9％ respectively.The cell proliferation activity in the transfection group was significantly lower than that in the non-transfection group,liposome group and negative vector group (all P＜0.05).The number of cell migration in the nontransfection group,negative vector group,liposome group and transfection group was 118.8 ±12.0,100.8±9.0,114.3 ±4.7,and 53.5±16.3 respectively.The differences were statistically significant between the transfection group and the other 3 groups (all P＜0.05).Conclusions PCDGF RNA interference can inhibit the proliferation and invasion abilities of esophageal squamous carcinoma cells in vitro.PCDGF gene may be the new target of gene therapy.
