中国医师杂志
中國醫師雜誌
중국의사잡지
JOURNAL OF CHINESE PHYSICIAN
2013年
z1期
5-7
,共3页
胡波%于晶功%孙静阳%裴笑月%李晓慧%孙海姣%王炜智
鬍波%于晶功%孫靜暘%裴笑月%李曉慧%孫海姣%王煒智
호파%우정공%손정양%배소월%리효혜%손해교%왕위지
P16%不典型鳞状上皮细胞%上皮内高度病变
P16%不典型鱗狀上皮細胞%上皮內高度病變
P16%불전형린상상피세포%상피내고도병변
P16%ASCUS%HSIL
目的 通过细胞块技术,检测P16蛋白在液基细胞学不典型鳞状上皮细胞(ASCUS)和宫颈上皮内高度病变(HSIL)中的表达,探讨P16蛋白在ASCUS重新评估中的意义.方法 收集本院2012年宫颈液基细胞学标本45例,其中15例细胞学诊断为ASCUS的病例,11例HSIL、11例宫颈上皮内低度病变(LSIL)和2例鳞癌、2例非典型腺细胞、4例正常细胞作为对照.细胞免疫化学染色,分析P16蛋白表达,将细胞学与组织学结果对照分析.结果 薄层液基细胞学涂片45例,P16蛋白在正常细胞、ASCUS、LSIL、HSIL、鳞癌、非典型腺细胞中的阳性表达率分别为0、20％、27.2％、63.6％、100％、100％.细胞学与活检结果,细胞学诊断ASCUS 15例,活检:宫颈炎12例,CINⅡ-Ⅲ3例;细胞学诊断LSIL11例,活检:宫颈炎5例,CIN Ⅰ 6例;细胞学诊断HSIL11例,活检:宫颈炎4例,CINⅡ-Ⅲ7例;细胞学诊断非典型腺细胞2例,活检:腺癌;细胞学诊断鳞癌2例,活检:鳞癌.结论 细胞块上检测P16蛋白可用于ASCUS再分类,帮助ASCUS重新评估.
目的 通過細胞塊技術,檢測P16蛋白在液基細胞學不典型鱗狀上皮細胞(ASCUS)和宮頸上皮內高度病變(HSIL)中的錶達,探討P16蛋白在ASCUS重新評估中的意義.方法 收集本院2012年宮頸液基細胞學標本45例,其中15例細胞學診斷為ASCUS的病例,11例HSIL、11例宮頸上皮內低度病變(LSIL)和2例鱗癌、2例非典型腺細胞、4例正常細胞作為對照.細胞免疫化學染色,分析P16蛋白錶達,將細胞學與組織學結果對照分析.結果 薄層液基細胞學塗片45例,P16蛋白在正常細胞、ASCUS、LSIL、HSIL、鱗癌、非典型腺細胞中的暘性錶達率分彆為0、20％、27.2％、63.6％、100％、100％.細胞學與活檢結果,細胞學診斷ASCUS 15例,活檢:宮頸炎12例,CINⅡ-Ⅲ3例;細胞學診斷LSIL11例,活檢:宮頸炎5例,CIN Ⅰ 6例;細胞學診斷HSIL11例,活檢:宮頸炎4例,CINⅡ-Ⅲ7例;細胞學診斷非典型腺細胞2例,活檢:腺癌;細胞學診斷鱗癌2例,活檢:鱗癌.結論 細胞塊上檢測P16蛋白可用于ASCUS再分類,幫助ASCUS重新評估.
목적 통과세포괴기술,검측P16단백재액기세포학불전형린상상피세포(ASCUS)화궁경상피내고도병변(HSIL)중적표체,탐토P16단백재ASCUS중신평고중적의의.방법 수집본원2012년궁경액기세포학표본45례,기중15례세포학진단위ASCUS적병례,11례HSIL、11례궁경상피내저도병변(LSIL)화2례린암、2례비전형선세포、4례정상세포작위대조.세포면역화학염색,분석P16단백표체,장세포학여조직학결과대조분석.결과 박층액기세포학도편45례,P16단백재정상세포、ASCUS、LSIL、HSIL、린암、비전형선세포중적양성표체솔분별위0、20％、27.2％、63.6％、100％、100％.세포학여활검결과,세포학진단ASCUS 15례,활검:궁경염12례,CINⅡ-Ⅲ3례;세포학진단LSIL11례,활검:궁경염5례,CIN Ⅰ 6례;세포학진단HSIL11례,활검:궁경염4례,CINⅡ-Ⅲ7례;세포학진단비전형선세포2례,활검:선암;세포학진단린암2례,활검:린암.결론 세포괴상검측P16단백가용우ASCUS재분류,방조ASCUS중신평고.
Objective Through the cell block technique to detect the expression of P16 protein in the liquid-based cytology with atypical squamous cells of undetermined significance (ASCUS) and high degree of cervical intraepithelial lesions (HSIL),to explore the significance of P16 protein in ASCUS re-evaluate.Methods Collected in our hospital in 2012 cervix liquid based cytology specimens of 45 patients,including of 15 ASCUS,11 HSIL cases,low in 11 cases of epithelial lesions (LSIL) and 2 cases of squamous cell carcinoma,2 cases of atypical glandular cells,4 cases of normal cells as a control.Immunocytochemical analysis of P16 protein control analysis,cytology and histology results.Results The expressing of P16 protein in normal cells,ASCUS,LSIL,HSIL,squamous cell carcinoma,atypical glandular cells in the positive expression rates were 0,20％,27.2％,63.6％,100％,100％.Cytology and biopsy results,cytologic diagnosis of ASCUS 15 cases,biopsy:12 cases of cervicitis,CIN Ⅱ-Ⅲ in 3 cases; cytology the in LSIL11,biopsy:5 cases of cervicitis,CIN Ⅰ 6 cases ; the cytological diagnosis HSIL11 cases,biopsy:cervical four cases of intlammation,CIN Ⅱ-Ⅲ ; cytologic diagnosis of atypical glandular cells in 2 cases,biopsy:adenocarcinoma; cytologic diagnosis of squamous cell carcinoma in 2 cases,biopsy:squamous cell carcinoma.Conclusion Detection of P16 protein on the cell block can be used for ASCUS classification ASCUS reassessment.
