中国医师杂志
中國醫師雜誌
중국의사잡지
JOURNAL OF CHINESE PHYSICIAN
2014年
8期
1052-1055
,共4页
贺彧彬%柳丽娜%卫政%吉鹏娟%申晓彧
賀彧彬%柳麗娜%衛政%吉鵬娟%申曉彧
하욱빈%류려나%위정%길붕연%신효욱
降血脂药/药理学%氟苯类/药理学%嘧啶类/药理学%磺胺类/药理学%联苯化合物/药理学%四唑类/药理学%咪唑类/药理学%脂蛋白类,LDL%单核巨噬细胞系统/药物作用%细胞质膜微囊蛋白/药物作用
降血脂藥/藥理學%氟苯類/藥理學%嘧啶類/藥理學%磺胺類/藥理學%聯苯化閤物/藥理學%四唑類/藥理學%咪唑類/藥理學%脂蛋白類,LDL%單覈巨噬細胞繫統/藥物作用%細胞質膜微囊蛋白/藥物作用
강혈지약/약이학%불분류/약이학%밀정류/약이학%광알류/약이학%련분화합물/약이학%사서류/약이학%미서류/약이학%지단백류,LDL%단핵거서세포계통/약물작용%세포질막미낭단백/약물작용
Antilipemic agents/pharmacology%Fluorobenzenes/pharmacology%Pyrimidines/pharmacology%Sulfonamides/pharmacology%Biphenyl compounds/pharmacology%Tetrazoles/pharmacology%Imidazoles/pharmacology%Lipoproteins,LDL%Mononuclear phagocyte system/dr
目的 探讨瑞舒伐他汀、氯沙坦以及二者联合对氧化修饰低密度脂蛋白(ox-LDL)诱导的人单核巨噬细胞小凹蛋白-1表达的影响.方法 分离培养人单核细胞并诱导转化为巨噬细胞,建立动脉粥样硬化模型,分别以不同剂量瑞舒伐他汀(0.1、1.0、5.0 μmol/L)、氯沙坦(10、50、100μmol/L)以及联合两药最适剂量(5.0 μmol/L+ 100 μmol/L)干预细胞,以RT-PCR检测各组小凹蛋白-1的mRNA表达.结果 与空白对照组比较,ox-LDL组小凹蛋白-1 mRNA表达显著减少[(0.2533±0.00973) vs (0.9410 ±0.03677)],其差异有统计学意义(P＜0.01);与ox-LDL组比较,随着瑞舒伐他汀及氯沙坦浓度的升高,小凹蛋白-1 mRNA表达均逐渐升高[(0.5198±0.04840)、(0.6183±0.06740)、(0.7257±0.03052) vs(0.2533±0.00973);(0.3350 ±0.04177)、(0.4428 ±0.03804)、(0.6049 ±0.02627) vs (0.2533 ±0.00973)],各组间比较差异有统计学意义(P＜0.05);最适剂量瑞舒伐他汀及氯沙坦联合组与单药组比较,小凹蛋白-1 mRNA表达显著升高,各组间比较差异均有统计学意义(F =59.119,P＜0.01).结论 瑞舒伐他汀及氯沙坦均可以通过ox-LDL途径上调人单核巨噬细胞小凹蛋白-1 mRNA表达,且呈一定的剂量依赖趋势;两药以最适剂量联合应用后,小凹蛋白-1 mRNA表达较单药更为显著.
目的 探討瑞舒伐他汀、氯沙坦以及二者聯閤對氧化脩飾低密度脂蛋白(ox-LDL)誘導的人單覈巨噬細胞小凹蛋白-1錶達的影響.方法 分離培養人單覈細胞併誘導轉化為巨噬細胞,建立動脈粥樣硬化模型,分彆以不同劑量瑞舒伐他汀(0.1、1.0、5.0 μmol/L)、氯沙坦(10、50、100μmol/L)以及聯閤兩藥最適劑量(5.0 μmol/L+ 100 μmol/L)榦預細胞,以RT-PCR檢測各組小凹蛋白-1的mRNA錶達.結果 與空白對照組比較,ox-LDL組小凹蛋白-1 mRNA錶達顯著減少[(0.2533±0.00973) vs (0.9410 ±0.03677)],其差異有統計學意義(P＜0.01);與ox-LDL組比較,隨著瑞舒伐他汀及氯沙坦濃度的升高,小凹蛋白-1 mRNA錶達均逐漸升高[(0.5198±0.04840)、(0.6183±0.06740)、(0.7257±0.03052) vs(0.2533±0.00973);(0.3350 ±0.04177)、(0.4428 ±0.03804)、(0.6049 ±0.02627) vs (0.2533 ±0.00973)],各組間比較差異有統計學意義(P＜0.05);最適劑量瑞舒伐他汀及氯沙坦聯閤組與單藥組比較,小凹蛋白-1 mRNA錶達顯著升高,各組間比較差異均有統計學意義(F =59.119,P＜0.01).結論 瑞舒伐他汀及氯沙坦均可以通過ox-LDL途徑上調人單覈巨噬細胞小凹蛋白-1 mRNA錶達,且呈一定的劑量依賴趨勢;兩藥以最適劑量聯閤應用後,小凹蛋白-1 mRNA錶達較單藥更為顯著.
목적 탐토서서벌타정、록사탄이급이자연합대양화수식저밀도지단백(ox-LDL)유도적인단핵거서세포소요단백-1표체적영향.방법 분리배양인단핵세포병유도전화위거서세포,건립동맥죽양경화모형,분별이불동제량서서벌타정(0.1、1.0、5.0 μmol/L)、록사탄(10、50、100μmol/L)이급연합량약최괄제량(5.0 μmol/L+ 100 μmol/L)간예세포,이RT-PCR검측각조소요단백-1적mRNA표체.결과 여공백대조조비교,ox-LDL조소요단백-1 mRNA표체현저감소[(0.2533±0.00973) vs (0.9410 ±0.03677)],기차이유통계학의의(P＜0.01);여ox-LDL조비교,수착서서벌타정급록사탄농도적승고,소요단백-1 mRNA표체균축점승고[(0.5198±0.04840)、(0.6183±0.06740)、(0.7257±0.03052) vs(0.2533±0.00973);(0.3350 ±0.04177)、(0.4428 ±0.03804)、(0.6049 ±0.02627) vs (0.2533 ±0.00973)],각조간비교차이유통계학의의(P＜0.05);최괄제량서서벌타정급록사탄연합조여단약조비교,소요단백-1 mRNA표체현저승고,각조간비교차이균유통계학의의(F =59.119,P＜0.01).결론 서서벌타정급록사탄균가이통과ox-LDL도경상조인단핵거서세포소요단백-1 mRNA표체,차정일정적제량의뢰추세;량약이최괄제량연합응용후,소요단백-1 mRNA표체교단약경위현저.
Objective To investigate the effects,mechanisms,and the optimum doses of Rosuvastatin and Losartan on expression of caveolin-1 in cultured human monocyte-macrophage cells which were induced by oxidized low density lipoprotein(ox-LDL).Methods Human-monocyte cells were separated and changed into the human monocyte-macrophage cells.The model of amerosclerosis was set up.These cells were incubated in different doses of Rosuvastatin(0.1,1.0,5.0 μmol/L) and Losartan (10,50,100 μmol/L),and then cultured in combination of two drags (5.0 μmol/L + 100 μmol/L).Expression of caveolin-1 mRNA was determined with real-time fluorescent quantitative polymerase chain reaction (RT-PCR).Results In ox-LDL group,caveolin-1 mRNA was decreased sharply relative to control group [(0.2533 ±0.00973) vs (0.9410 ±0.03677)] in a concentration-dependent manner (P ＜0.01).Compared to ox-LDL group,expressions of Caveolin-1 mRNA were increased gradually in different doses of Rosuvastatin alone and Losartan alone group [(0.5198 ± 0.04840),(0.6183 ± 0.06740),(0.7257 ± 0.03052) vs (0.2533 ± 0.00973) ; (0.3350 ± 0.04177),(0.4428 ± 0.03804),(0.6049 ± 0.02627) vs (0.2533 ± 0.00973)] in a concentration-dependent manner (P ＜ 0.01) ; the summit expressions of caveolin-1 mRNA were emerged in using Rosuvastatin and Losartan together (F =59.119,P ＜ 0.01).Conclusions Rosuvastatin and Losartan may be responsible for the expression of caveolin-1 in human monocyte-macrophage cells that were induced by ox-LDL.The expressions were up-regulated with dose dependent manner of these drugs,and got the crest stage when using optimum doses of Rosuvastatin and Losartan together.
