CAJ | 학술논문

目的探讨miR-451是否通过靶向调控巨噬细胞移动抑制因子(MIF)表达抑制胃癌细胞的迁移和侵袭,从而进一步揭示miR-451的抑瘤机制.方法运用qRT-PCR检测72例胃癌组织及癌旁正常胃黏膜组织中miR-451的表达改变;采用Western blot检测外源高表达miR-451对MIF蛋白表达水平的影响;然后采用划痕实验和Transwell侵袭实验检测外源高表达miR-451对人胃癌MGC-803细胞迁移和侵袭的影响.结果 qRT-PCR检测结果显示,miR-451在72例胃癌组织中的表达水平较癌旁正常胃黏膜组织明显下调,miR-451的表达水平与胃癌患者的临床分期和淋巴结转移呈负相关;Western blot结果显示,过表达miR-451能下调MIF蛋白的表达.划痕实验和Transwell侵袭实验检测发现,过表达miR-451能抑制MGC-803细胞迁移和侵袭能力,差异均具有统计学意义(P＜0.05).结论 miR-451通过靶向调控MIF的表达抑制胃癌细胞的迁移和侵袭.
목적 탐토miR-451시부통과파향조공거서세포이동억제인자(MIF)표체억제위암세포적천이화침습,종이진일보게시miR-451적억류궤제.방법 운용qRT-PCR검측72례위암조직급암방정상위점막조직중miR-451적표체개변;채용Western blot검측외원고표체miR-451대MIF단백표체수평적영향;연후채용화흔실험화Transwell침습실험검측외원고표체miR-451대인위암MGC-803세포천이화침습적영향.결과 qRT-PCR검측결과현시,miR-451재72례위암조직중적표체수평교암방정상위점막조직명현하조,miR-451적표체수평여위암환자적림상분기화림파결전이정부상관;Western blot결과현시,과표체miR-451능하조MIF단백적표체.화흔실험화Transwell침습실험검측발현,과표체miR-451능억제MGC-803세포천이화침습능력,차이균구유통계학의의(P＜0.05).결론 miR-451통과파향조공MIF적표체억제위암세포적천이화침습.
Objective To explore whether miR-451 suppresses cell migration and invasion by targeting macrophage migration inhibitory factors (MIF),thus to reveal molecular mechanism that miR-451 functions as a tumor suppressor in gastric cancer.Methods A quantitative real-time polymerase chain reaction (qRT-PCR) was carried out to detect the expression of miR-451 in gastric cancer and normal stomach mucosa.Western blotting was performed to detect the expressions of MIF protein regulated by miR-451 in MGC 803 cells.The migration and invasion abilities of MGC-803 cell were evaluated with wound-healing and transwell invasion assays.Results miR-451 was down-regulated in gastric cancer tissues.The expressions of miR-451 were negatively correlated with the clinical stage and lymph node metastasis in gastric cancer patients.Western blot showed that a notable reduction of the protein level of MIF by restoring miR-451 in MGC-803 cells.Overexpression of miR-451 inhibited the migration and invasion of MGC-803 cells.Conclusions miR-451 suppresses cell migration and invasion by targeting MIF in gastric cancer.