CAJ | 학술논문

目的探讨碳酸酐酶1(CA1)在不同临床分期结直肠癌组织中的表达模式及其与结直肠癌患者临床病理特征的相关性.方法采用实时定量PCR检测基因水平CA1的表达.分别采用蛋白免疫印迹和免疫组织化学染色法检测蛋白水平CA1的表达.结果在62例冰冻组织样本中,基因水平及蛋白水平的CA1在癌远端正常结直肠黏膜中的表达显著高于癌组织(P＜0.01),不同临床分期结直肠癌组织中CA1表达呈折线模式,基因水平CA1的表达模式与蛋白水平相似,且均在进展期(Ⅲ期+Ⅳ期)结直肠癌组织中下调更为明显.免疫组化在103例石蜡包埋组织中检测发现,CA1在癌远端正常上皮细胞中阳性表达率90.29％,显著高于癌组织中的阳性表达率(62.14％,P＜0.01).CA1在高分化、中分化及低分化结直肠癌组织中的阳性表达率分别为90％、62.79％和5％,两两比较差异具有统计学意义(P＜0.01).在存在淋巴结及远端转移的结直肠癌组织中CA1表达显著低于无转移组(37.14％vs 75％,P＜0.01).CA1表达缺失与结直肠癌患者预后差相关,CA1阴性表达组5年生存率仅为8％,而阳性表达组患者5年生存率为92.86％,两者比较差异具有统计学意义(P＜0.01).结论 CA1在基因水平和蛋白水平具有相似的表达模式.在结直肠癌组织中表达下调甚至缺失.CA1的表达下调与患者存在淋巴结转移或远处转移有关.癌组织分化程度越低,CA1表达下调越明显,越易发生转移,预后越差.
목적 탐토탄산항매1(CA1)재불동림상분기결직장암조직중적표체모식급기여결직장암환자림상병리특정적상관성.방법 채용실시정량PCR검측기인수평CA1적표체.분별채용단백면역인적화면역조직화학염색법검측단백수평CA1적표체.결과 재62례빙동조직양본중,기인수평급단백수평적CA1재암원단정상결직장점막중적표체현저고우암조직(P＜0.01),불동림상분기결직장암조직중CA1표체정절선모식,기인수평CA1적표체모식여단백수평상사,차균재진전기(Ⅲ기+Ⅳ기)결직장암조직중하조경위명현.면역조화재103례석사포매조직중검측발현,CA1재암원단정상상피세포중양성표체솔90.29％,현저고우암조직중적양성표체솔(62.14％,P＜0.01).CA1재고분화、중분화급저분화결직장암조직중적양성표체솔분별위90％、62.79％화5％,량량비교차이구유통계학의의(P＜0.01).재존재림파결급원단전이적결직장암조직중CA1표체현저저우무전이조(37.14％vs 75％,P＜0.01).CA1표체결실여결직장암환자예후차상관,CA1음성표체조5년생존솔부위8％,이양성표체조환자5년생존솔위92.86％,량자비교차이구유통계학의의(P＜0.01).결론 CA1재기인수평화단백수평구유상사적표체모식.재결직장암조직중표체하조심지결실.CA1적표체하조여환자존재림파결전이혹원처전이유관.암조직분화정도월저,CA1표체하조월명현,월역발생전이,예후월차.
Objective To investigate the expression pattern of carbonic anhydrase 1 (CA1) in different clinical stages of color ectal cancers and the correlation between CA1 expression and pathologic characteristics of colorectal cancer.Methods The expression of CA1 in genetic level was detected by real-time quantitative polymerase chain reaction (PCR) and the expression of CA1 in protein level was detected by Western blot and immunohistochemical staining.Results The expression pattern of CA1 in the protein level was very similar to the genetic level.It had a very low expression level in carcinoma tissue,especially at stages Ⅲ and Ⅳ (P ＜0.01),CA1 had significantly lower expression level in the colorectal patients with metastasis (P ＜0.01).CA1 was gradually decreased from well-differentiation tissue to poorly-differentiation tissue with a significant difference between well-differentiated adenocarcinoma and poorly-differentiated adenocarcinoma (P ＜ 0.01).Kaplan-Meier survival analysis showed that the expression of CA1 was extremely related to colorectal carcinoma (CRC) prognosis.The 5-year survival rate was only 8％ in the CA1 negative expression group,and the 5-year survival rate of the patients with CA1 positive expression was 92.86％ (P ＜ 0.01).Conclusions CA1 had the similar expression patterns at genetic and protein levels.In the colorectal cancer tissue,the expression of CA1 was downregulated and even missing.CA1 had significantly lower expression level in the advanced colorectal cancers with metastasis and poorly differentiated colorectal cancer tissues.The patients with lower CA1 expression level had the worse prognosis.