CAJ | 학술논문

目的探讨鞘内移植骨髓基质细胞(BMSCs)对大鼠脊髓缺血再灌注损伤后细胞间黏附分子-1(ICAM-1)表达和血脊髓屏障的影响.方法 SD大鼠90只,按随机数字表法分为3组,每组30只.假手术组(Sham组)、缺血再灌注损伤组(I/R组)和BMSCs移植组(BMSCs组).I/R组和BMSCs组阻断左颈总动脉和左锁骨下动脉之间主动脉弓14 min建立脊髓缺血再灌注损伤模型,Sham组暴露主动脉弓,但不阻断.损伤前2d蛛网膜下腔置管,BMSCs组注入2×106 BMSCs,I/R组注入等容量PBS.分别于再灌注损伤后1、3和7d进行神经功能评分,取脊髓损伤腰骶节段,western blot测定脊髓组织中ICAM-1表达水平,并根据伊文思蓝外渗情况测定血脊髓屏障通透性.结果与Sham组比较,I/R组神经运动功能评分降低:1 d(F=38.59,P=0.001),3 d(F =31.34,P=0.001),7d(F =27.71,P=0.001);脊髓组织ICAM-1表达增加:1 d(F=34.33,P =0.001),3 d(F=29.76,P=0.001),7 d(F=23.65,P=0.001);血脊髓屏障通透性增加:1 d(F=42.57,P=0.001),3 d(F =32.75,P=0.001),7d(F =26.89,P=0.001).与I/R组比较,BMSCs组神经运动功能评分增高:1 d(F=16.62,P =0.001),3d(F=21.54,P=0.001),7d(F=12.84,P=0.002);脊髓组织ICAM-1表达减少:1 d(F=19.84,P=0.018),3d(F =17.38,P=0.008),7d(F =22.46,P=0.007);血脊髓屏障通透性降低:1 d(F=22.38,P=0.016),3d(F =27.59,P=0.009),7 d(F=23.25,P=0.001).结论鞘内移植骨髓基质细胞能够抑制ICAM-1表达,保护血脊髓屏障,降低血脊髓屏障通透性,进而降低脊髓缺血再灌注损伤.
목적 탐토초내이식골수기질세포(BMSCs)대대서척수결혈재관주손상후세포간점부분자-1(ICAM-1)표체화혈척수병장적영향.방법 SD대서90지,안수궤수자표법분위3조,매조30지.가수술조(Sham조)、결혈재관주손상조(I/R조)화BMSCs이식조(BMSCs조).I/R조화BMSCs조조단좌경총동맥화좌쇄골하동맥지간주동맥궁14 min건립척수결혈재관주손상모형,Sham조폭로주동맥궁,단불조단.손상전2d주망막하강치관,BMSCs조주입2×106 BMSCs,I/R조주입등용량PBS.분별우재관주손상후1、3화7d진행신경공능평분,취척수손상요저절단,western blot측정척수조직중ICAM-1표체수평,병근거이문사람외삼정황측정혈척수병장통투성.결과 여Sham조비교,I/R조신경운동공능평분강저:1 d(F=38.59,P=0.001),3 d(F =31.34,P=0.001),7d(F =27.71,P=0.001);척수조직ICAM-1표체증가:1 d(F=34.33,P =0.001),3 d(F=29.76,P=0.001),7 d(F=23.65,P=0.001);혈척수병장통투성증가:1 d(F=42.57,P=0.001),3 d(F =32.75,P=0.001),7d(F =26.89,P=0.001).여I/R조비교,BMSCs조신경운동공능평분증고:1 d(F=16.62,P =0.001),3d(F=21.54,P=0.001),7d(F=12.84,P=0.002);척수조직ICAM-1표체감소:1 d(F=19.84,P=0.018),3d(F =17.38,P=0.008),7d(F =22.46,P=0.007);혈척수병장통투성강저:1 d(F=22.38,P=0.016),3d(F =27.59,P=0.009),7 d(F=23.25,P=0.001).결론 초내이식골수기질세포능구억제ICAM-1표체,보호혈척수병장,강저혈척수병장통투성,진이강저척수결혈재관주손상.
Objective To investigate the effects of intrathecal transplantation of bone marrow stromal cells (BMSCs) on inter cellular cell adhesion molecule-1 (ICAM-1) expression and blood spinal cord barrier following spinal cord ischemia reperfusion injury.Methods Ninety Sprague Dawley rats were randomly divided into three groups:sham (Sham group),ischemia-reperfusion injury (I/ R group),and BMSCs transplantation (BMSCs group).Spinal I/R injury was induced by clamping the aortic arch between left common carotid artery and left subclavian artery for 14 min in I/R group and BMSCs group.Sham group was subjected to exposure of aortic arch but without occlusion.I/R group and BMSCs group were intrathecally injected with phosphate buffered saline (PBS) or BMSCs (2 × 106) two days before injury.At 1 d,3 d,and 7 d after injury,neurological function was evaluated and damaged lumbosacral seg ment was removed for measurement of blood spinal cord barrier permeability and ICAM-1 protein expression.Results Compared with Sham group,neurological function score was significantly lower:1 d (F =38:59,P =0.001),3 d (F =31.34,P =0.001),and 7 d (F =27.71,P =0.001) ; ICAM-1 expression was increased 1 d (F =34.33,P =0.001),3 d (F =29.76,P =0.001),and 7 d (F =23.65,P =0.001),and blood spinal cord barrier permeability was higher:1 d (F =42.57,P =0.001),3 d (F =32.75,P =0.001),and 7 d (F =26.89,P =0.001) in I/R group.Compared with I/R group,neurological function score was increased:1 d (F =16.62,P =0.001),3 d (F =21.54,P =0.001),and 7 d (F =12.84,P =0.002) ; ICAM-1 expression was decreased:1 d (F =19.84,P =0.018),3 d (F =17.38,P =0.008),and 7 d (F =22.46,P =0.007),and blood spinal cord barrier permeability was lower:1 d (F =22.38,P =0.016),3 d (F =27.59,P =0.009),and 7 d (F =23.25,P =0.001) in BMSCs group.Conclusions Intrathecal transplantation of BMSCs inhibited ICAM-1 expression and decreased blood spinal cord barrier permeability,and then attenuated spinal cord ischemia-reperfusion injury.