CAJ | 학술논문

目的探讨高脂饮食对胰腺腺泡细胞内三磷酸肌醇( IP3)表达及淀粉酶释放的影响.方法雄性Wistar大鼠分为高脂饮食组和正常饮食组,分别喂养4周,全自动生化仪检测血液甘油三酯、胆固醇、淀粉酶和葡萄糖浓度,并观察胰腺病理组织学变化.两组大鼠分离并培养胰腺腺泡细胞,应用[3H]-IP3检测试剂盒检测细胞内IP3浓度；并应用胆囊收缩素(CCK)-8对腺泡细胞刺激培养,检测两组大鼠腺泡细胞淀粉酶释放率.结果高脂饮食大鼠出现高脂血症,其胰腺组织病理染色发现腺泡细胞空泡化,腺泡细胞周围淋巴细胞浸润.高脂饮食大鼠的腺泡细胞IP3含量明显高于正常大鼠[(31.807±3.448) pmol/106 cells与(24.632±3.649) pmol/106 cells,t=7.479,P＜0.001]；且高脂饮食大鼠较正常组大鼠的腺泡细胞在不同浓度CCK-8(0.01 nmol/L和1 nmol/L)刺激下的淀粉酶释放率均明显增高[(11.056±3.369)％与(7.354±2.181)％;t=3.912,P＜0.001;(13.854±4.087)％与(9.432±2.477)％,t=3.939,P＜0.001).高脂饮食组大鼠的腺泡细胞IP3含量和CCK-8(1 nmol/L)刺激下的淀粉酶释放率呈正相关性(r=0.896,P＜0.001).结论长期高脂血症引起大鼠腺泡细胞对CCK刺激的外分泌敏感性增加,IP3作为信号分子具有重要作用.
목적 탐토고지음식대이선선포세포내삼린산기순( IP3)표체급정분매석방적영향.방법 웅성Wistar대서분위고지음식조화정상음식조,분별위양4주,전자동생화의검측혈액감유삼지、담고순、정분매화포도당농도,병관찰이선병리조직학변화.량조대서분리병배양이선선포세포,응용[3H]-IP3검측시제합검측세포내IP3농도；병응용담낭수축소(CCK)-8대선포세포자격배양,검측량조대서선포세포정분매석방솔.결과 고지음식대서출현고지혈증,기이선조직병리염색발현선포세포공포화,선포세포주위림파세포침윤.고지음식대서적선포세포IP3함량명현고우정상대서[(31.807±3.448) pmol/106 cells여(24.632±3.649) pmol/106 cells,t=7.479,P＜0.001]；차고지음식대서교정상조대서적선포세포재불동농도CCK-8(0.01 nmol/L화1 nmol/L)자격하적정분매석방솔균명현증고[(11.056±3.369)％여(7.354±2.181)％;t=3.912,P＜0.001;(13.854±4.087)％여(9.432±2.477)％,t=3.939,P＜0.001).고지음식조대서적선포세포IP3함량화CCK-8(1 nmol/L)자격하적정분매석방솔정정상관성(r=0.896,P＜0.001).결론 장기고지혈증인기대서선포세포대CCK자격적외분비민감성증가,IP3작위신호분자구유중요작용.
Objective To investigate the effects of high-fat diet on pancreatic acinar cells' IP3 expression and CCK-induced amylase release in rats.Methods Male Wistar rats were divided into high-fat diet group and normal diet group,they were fed for 4 weeks.Blood triglycerides,cholesterol,amylase and glucose levels were determined by automatic biochemical analyzer.Pancreatic tissues were taken for histopathological observations.Pancreatic acinar cells were isolated and cultured,and intracellular inositol 1,4,5-trisphosphate (IP3) was detected using a commercial kit.Amylase release rates were measured after CCK-8 stimulation.Results The rats in high-fat diet group appeared hyperlipidemia,vacuolization of acinar cells and the lymphocytes appeared around the acinar cells can be seen on the pancreatic tissue pathology staining.The levels of IP3 in acinar cells of rats fed a high-fat diet were higher than that of normal rats [(31.807 ± 3.448) pmol/106 cells vs (24.632 ± 3.649) pmol/106 cells,t=7.479,P＜0.001];and amylase release rate in these rats'acinar cells were also higher than those of normal rats [when CCK-8=0.01 nmol/L:( 11.056 ±3.369)％ vs (7.354 ± 2.181) ％,t=3.912,P＜0.001;when CCK-8=1 nmol/L:( 13.854 ± 4.087 ) ％ vs (9.432 ±2.477) ％,t=3.939,P＜0.001 ) after CCK-8 stimulation in different concentrations.Additionally,there was a positive co-relationship between acinar cell's IP3 level and amylase release (r=0.896,P＜0.001 ).Conclusion Chronic high-fat diet induces hypersensitivity for pancreatic acinar cells' exocrine function,and IP3 as a signal molecule may play an important role in this process.