CAJ | 학술논문

目的体外观察葡萄球菌肠毒素A(SEA)对人肝癌细胞株SSMC7721的增殖抑制作用,旨在为肝癌的治疗提供理论依据.方法应用噻唑蓝(MTT)比色法检测不同浓度(0、20、40、80 μg/L)的SEA对SSMC7721细胞的增殖抑制率,流式细胞仪检测细胞周期进程的变化,荧光显微镜检测细胞凋亡情况.结果 0、20、40、80.μg/L浓度的SEA吸光度(A)值分别为1.52±0.12,1.14±0.13,0.98±0.16,0.82±0.21;根据吸光度值计算SSMC7721细胞增殖抑制率分别为0、(18.4±1.3)％、(35.5±0.9)％、(46.7±1.6)％,多组间经统计学处理,差异有统计学意义(F=4.63,P＜0.05),其他3组与0μg/L组比较,差异均有统计学意义(P均＜0.01)；显示不同浓度的SEA对SSMC7721细胞增殖均有抑制作用,且呈剂量依赖性.流式细胞仪检测结果显示:不同浓度的SEA对SSMC7721细胞均有影响,G0/G1期细胞增多,S期细胞减少,G2/M期细胞相对增多；荧光染色结果显示正常SSMC7721细胞凋亡率为1.0％,而20、40、80μg/L浓度的SEA对SSMC7721细胞凋亡率分别为12.5％、19.4％和23.2％.结论 SEA能显著抑制SSMC7721细胞增殖,抑制SSMC7721细胞Gl期向S期转化进程,诱导SSMC7721细胞凋亡.
목적 체외관찰포도구균장독소A(SEA)대인간암세포주SSMC7721적증식억제작용,지재위간암적치료제공이론의거.방법 응용새서람(MTT)비색법검측불동농도(0、20、40、80 μg/L)적SEA대SSMC7721세포적증식억제솔,류식세포의검측세포주기진정적변화,형광현미경검측세포조망정황.결과 0、20、40、80.μg/L농도적SEA흡광도(A)치분별위1.52±0.12,1.14±0.13,0.98±0.16,0.82±0.21;근거흡광도치계산SSMC7721세포증식억제솔분별위0、(18.4±1.3)％、(35.5±0.9)％、(46.7±1.6)％,다조간경통계학처리,차이유통계학의의(F=4.63,P＜0.05),기타3조여0μg/L조비교,차이균유통계학의의(P균＜0.01)；현시불동농도적SEA대SSMC7721세포증식균유억제작용,차정제량의뢰성.류식세포의검측결과현시:불동농도적SEA대SSMC7721세포균유영향,G0/G1기세포증다,S기세포감소,G2/M기세포상대증다；형광염색결과현시정상SSMC7721세포조망솔위1.0％,이20、40、80μg/L농도적SEA대SSMC7721세포조망솔분별위12.5％、19.4％화23.2％.결론 SEA능현저억제SSMC7721세포증식,억제SSMC7721세포Gl기향S기전화진정,유도SSMC7721세포조망.
Objective We detected the proliferation inhibition effect of staphylococcal enterotoxin A (SEA) on human liver cancer SSMC7721 cell lines in vitro to provide a theoretical basis for the therapy of liver cancer.Methods We applied MTT colorimetric method to assay the SSMC7721 cell's proliferation inhibition rate treated by different concentrations (0 μg/L,20 μg/L,40 μg/L and 80 μL) of SEA.We decided the changes of the cell cycle progression by flow cytometry and the apoptosis rate by fluorescence microscopy.Results When the SSMC7721 cells were treated with different concentrations(20 μg/L,40 μg/L and 80 μg/L) of SEA,cell proliferation inhibition rates were (18.4 ± 1.3) ％,(35.5 ± 0.9) ％,(46.7 ±1.6) ％ respectively and the statistical differences were significant compared to the control group(P ＜ 0.01),and the proliferation is in an obvious dose dependent manner.Flow cytometry results indicated that the cells in the G0/G1 period increased;the cells in the S period decreased;the cells in the G2/M period increased.Cell apoptosis rates after treatment by 20 μg/L,40 μg/L and 80 μg/L SEA increased to 12.5％,19.4％ and 23.2％.respectively from 1.0％ without SEA treatment.Conclusion SEA can significantly increase the cells proliferation inhibition rate and inhibit the cell's transformation from G1 to S period in SSMC7721 cell and induce the SSMC7721 cell's apoptosis.