中国综合临床
中國綜閤臨床
중국종합림상
CLINICAL MEDICINE OF CHINA
2013年
3期
225-228
,共4页
聂丽萍%穆维新%沈亚欣%郭庆军
聶麗萍%穆維新%瀋亞訢%郭慶軍
섭려평%목유신%침아흔%곽경군
罗格列酮%糖尿病%白细胞介素6%白细胞介素10
囉格列酮%糖尿病%白細胞介素6%白細胞介素10
라격렬동%당뇨병%백세포개소6%백세포개소10
Rosiglitazone%Diabetes mellitus%Interlukin-6%Interlukin-10
目的 观察罗格列酮对糖尿病大鼠肺组织中白细胞介素(IL)-6、IL-10的影响.方法 通过高糖、高脂饮食加腹腔注射小剂量链尿佐菌素(STZ)的方法,建立2型糖尿病大鼠模型,采用光镜动态观察10、20周时,对照组(8只)、单纯糖尿病组(8只)、罗格列酮治疗组(7只)大鼠肺组织的病理改变情况,并用免疫组织化学方法观察IL-6、IL-10的表达.结果 对照组、单纯糖尿病组、罗格列酮治疗组10、20周IL-6分别为0.15±0.01、0.16±0.01与0.22±0.02、0.31±0.04和0.22±0.03、0.20±0.02；统计结果为F组内=216.89,P＜ 0.01；F组间=342.62,P＜0.01；F交互 =341.51,P＜0.01.除对照组10周与20周比较差异无统计学意义(P＞0.05)外,其他任意两组的比较差异均有统计学意义(P均＜0.05).对照组、单纯糖尿病组、罗格列酮治疗组10、20周IL-10分别为0.13±0.01、0.15±0.02与0.20±0.01、0.21±0.01和0.20±0.02、0.17±0.01;统计结果为F组内=14.612,P＜0.01；F组间=909.19,P＜0.01;F交互=210.55,P＜0.01.对照组10周与20周IL-10比较差异无统计学意义,其他任意两组IL-10的比较差异均有统计学意义(P均＜0.05).结论 糖尿病大鼠肺组织中IL-6、IL-10升高可能是导致糖尿病大鼠肺部炎症损伤原因之一,罗格列酮可通过调控IL-6、IL-10来减轻糖尿病大鼠肺组织的炎症病理变化.
目的 觀察囉格列酮對糖尿病大鼠肺組織中白細胞介素(IL)-6、IL-10的影響.方法 通過高糖、高脂飲食加腹腔註射小劑量鏈尿佐菌素(STZ)的方法,建立2型糖尿病大鼠模型,採用光鏡動態觀察10、20週時,對照組(8隻)、單純糖尿病組(8隻)、囉格列酮治療組(7隻)大鼠肺組織的病理改變情況,併用免疫組織化學方法觀察IL-6、IL-10的錶達.結果 對照組、單純糖尿病組、囉格列酮治療組10、20週IL-6分彆為0.15±0.01、0.16±0.01與0.22±0.02、0.31±0.04和0.22±0.03、0.20±0.02；統計結果為F組內=216.89,P＜ 0.01；F組間=342.62,P＜0.01；F交互 =341.51,P＜0.01.除對照組10週與20週比較差異無統計學意義(P＞0.05)外,其他任意兩組的比較差異均有統計學意義(P均＜0.05).對照組、單純糖尿病組、囉格列酮治療組10、20週IL-10分彆為0.13±0.01、0.15±0.02與0.20±0.01、0.21±0.01和0.20±0.02、0.17±0.01;統計結果為F組內=14.612,P＜0.01；F組間=909.19,P＜0.01;F交互=210.55,P＜0.01.對照組10週與20週IL-10比較差異無統計學意義,其他任意兩組IL-10的比較差異均有統計學意義(P均＜0.05).結論 糖尿病大鼠肺組織中IL-6、IL-10升高可能是導緻糖尿病大鼠肺部炎癥損傷原因之一,囉格列酮可通過調控IL-6、IL-10來減輕糖尿病大鼠肺組織的炎癥病理變化.
목적 관찰라격렬동대당뇨병대서폐조직중백세포개소(IL)-6、IL-10적영향.방법 통과고당、고지음식가복강주사소제량련뇨좌균소(STZ)적방법,건립2형당뇨병대서모형,채용광경동태관찰10、20주시,대조조(8지)、단순당뇨병조(8지)、라격렬동치료조(7지)대서폐조직적병리개변정황,병용면역조직화학방법관찰IL-6、IL-10적표체.결과 대조조、단순당뇨병조、라격렬동치료조10、20주IL-6분별위0.15±0.01、0.16±0.01여0.22±0.02、0.31±0.04화0.22±0.03、0.20±0.02；통계결과위F조내=216.89,P＜ 0.01；F조간=342.62,P＜0.01；F교호 =341.51,P＜0.01.제대조조10주여20주비교차이무통계학의의(P＞0.05)외,기타임의량조적비교차이균유통계학의의(P균＜0.05).대조조、단순당뇨병조、라격렬동치료조10、20주IL-10분별위0.13±0.01、0.15±0.02여0.20±0.01、0.21±0.01화0.20±0.02、0.17±0.01;통계결과위F조내=14.612,P＜0.01；F조간=909.19,P＜0.01;F교호=210.55,P＜0.01.대조조10주여20주IL-10비교차이무통계학의의,기타임의량조IL-10적비교차이균유통계학의의(P균＜0.05).결론 당뇨병대서폐조직중IL-6、IL-10승고가능시도치당뇨병대서폐부염증손상원인지일,라격렬동가통과조공IL-6、IL-10래감경당뇨병대서폐조직적염증병리변화.
Objective To observe the effect of rosiglitazone on the concentration of interlukin (IL)-6 and IL-10 in lung tissues of diabetic rats.Methods The experimental diabetic rats were yielded by injecting streptozotocin(STZ) and feeding with high fat and high glucose food.We observed lung morphology in control group,diabetes mellitus(DM) group,and rosiglitazone group at 10 week and 20 week respectively under light microscope.Alteration of IL-6 and IL-10 in lung was measured by immunohistochemistry.Results The optical density values of IL-6 in the control group,the DM group and the roggerosiglitazone treatment group were 0.15 ±0.01,0.16 ±0.01;0.22 ±0.02,0.31 ±0.04;0.22 ±0.03,and 0.20 ±0.02 at 10 week and 20 week respectively (Fwithin =216.89,P ＜ 0.01 ; Fbetween =342.62,P ＜ 0.01 ; Finteraction =341.51,P ＜ 0.01).Any two groups had significant difference(P ＜ 0.05) except the comparison of the IL-6 values at 10 week and 20 week in the control group (P ＞ 0.05).The absorbance values of IL-10 in the three groups were 0.13 ± 0.01,0.15 ±0.02;0.20 ±0.01,0.21 ±0.01;0.20 ±0.02,and 0.17 ±0.01 at 10 week and 20 week respectively (Fwithin =14.612,P ＜0.01 ;Fbetween =909.19,P ＜0.01 ;Finteraction =210.55,P ＜0.01).Any two groups had significant difference(P ＜0.05) except the comparison of the IL-6 values at 10 week and 20 week in the control group.Conclusion The elevated levels of IL-6 and IL-10 in lung tissue of dibtetic rats might be related to the inflammation of lung tissues.Rosiglitazone may alleviate lung inflammation by regulating the levels of IL-6 and IL-10.
