CAJ | 학술논문

目的探讨肾综合征出血热(HFRS)时血管内皮细胞的功能变化.方法用出血时间测定器、酶联免疫吸附、放射免疫分析、发色底物及胶乳凝集试验检测出血时间(BT)、血管性血友病因子抗原(vWF:Ag)、6-酮PGF1α(6-Keto)、组织纤溶酶原激活物活性(t-PA)、纤溶酶原激活抑制物-1活性(PAI-1:A)、内皮素-1(ET-1)、血纤维蛋白(原)降解产物(FDP).结果除轻型患者的t-PA:A、PAI-1:A、ET-1外,其他各型患者所测的指标与正常对照组相比差异均有显著性(P＜0.05,P＜0.001).各指标的动态观察发现除个别轻型患者外,vWF:Ag、ET-1、t-PA及FDP的血浆水平均随病程的进展而升高,6-酮-PGF1α在发病初期即有显著下降,休克期达最低,并随病情的缓解而趋向正常.结论在肾综合征出血热时,内皮细胞存在不同程度的损伤.
목적 탐토신종합정출혈열(HFRS)시혈관내피세포적공능변화.방법 용출혈시간측정기、매련면역흡부、방사면역분석、발색저물급효유응집시험검측출혈시간(BT)、혈관성혈우병인자항원(vWF:Ag)、6-동PGF1α(6-Keto)、조직섬용매원격활물활성(t-PA)、섬용매원격활억제물-1활성(PAI-1:A)、내피소-1(ET-1)、혈섬유단백(원)강해산물(FDP).결과 제경형환자적t-PA:A、PAI-1:A、ET-1외,기타각형환자소측적지표여정상대조조상비차이균유현저성(P＜0.05,P＜0.001).각지표적동태관찰발현제개별경형환자외,vWF:Ag、ET-1、t-PA급FDP적혈장수평균수병정적진전이승고,6-동-PGF1α재발병초기즉유현저하강,휴극기체최저,병수병정적완해이추향정상.결론 재신종합정출혈열시,내피세포존재불동정도적손상.
Objective To study the functional change of vascular endothdiocytes in hemorrhage fever with renal syndrome(HFRS).Methods The bleeding time(BT),von Winebrand Factor antigen (vWF:Ag),6-keto-PGF1α(6-Keto),tissue-type plasminogen activator(t-PA),plasminogen activator inhibitor-1 activity(PAI-1:A),endothelin-1(ET-1)and fibrin degradation product(FDP)were measured using template method,ELISA,radioimmunology analysis,color substrate and latex aggregating test.Resuits All the above parameters varied significantly comparing the normal controls(P＜0.05～0.001)except for t-PA:A,PAI-1:A and ET-1 in mild patients.vWF:Ag,ET-1,t-PA:A and FDP were progressively increasing as the disease proceeded except for some mild patients.6-Keto decreased significantly in the early stage,teached bottom in the shock stage and gradually returned to normal when the patients recovefed.Conclusions Damages in vascular endotheliocytes of various extents exist in HFRS patients.