CAJ | 학술논문

目的建立套式-聚合酶链反应(Nested-PCR)加限制性酶切分析方法检测人巨细胞病毒(HCMV).方法在HCMV直接早期蛋白EcoRIJ DNA片段内,自行设计两对引物,建立Nested-PCR检测HCMV DNA,同时结合病毒分离检测临床标本.结果 23例新生儿肝炎综合征患儿中,10例病毒分离及Nested-PCR均阳性;1例病毒分离阴性,但Nested-PCR阳性.对58例妊娠早期孕妇血标本进行检测,Nested-PCR阳性率为9%,病毒分离阳性率则为7%.结论 Nested-PCR加限制性酶切分析是一种临床检测HCMV的快速有效手段.
목적 건립투식-취합매련반응(Nested-PCR)가한제성매절분석방법검측인거세포병독(HCMV).방법 재HCMV직접조기단백EcoRIJ DNA편단내,자행설계량대인물,건립Nested-PCR검측HCMV DNA,동시결합병독분리검측림상표본.결과 23례신생인간염종합정환인중,10례병독분리급Nested-PCR균양성;1례병독분리음성,단Nested-PCR양성.대58례임신조기잉부혈표본진행검측,Nested-PCR양성솔위9%,병독분리양성솔칙위7%.결론 Nested-PCR가한제성매절분석시일충림상검측HCMV적쾌속유효수단.
Objective To establish an analytic method which combines nested-PCR and restriction enzyme analysis to detect human cytomegalovirus(HCMV).Methods We designed two primers according to the sequence of HCMV immediate early protein EcoRIJ gene to detect HCMV-DNA using nested-PCR.Meanwhile,virus isolations were also conducted to test clinical samples. Results Among 23 cases of neonatal hepatitis syndrome,HCMV was found in 10 cases using nested-PCR and virus isolation.One case showed onlv positive with nested-PCR.Among 58 cases of early gravida,the positive rates of nested-PCR and virus isolation were 8.6%and 6.9%respectively.Conclusions Nested-PCR combined with restriction enzyme analysis would be effective in rapid detection of HCMV.