我国各地区动物狂犬病街毒的检测及分析
아국각지구동물광견병가독적검측급분석
Detection and analysis of rabies street virus in animals in China
  • 万方数据
    中华传染病杂志 중화전염병잡지
    CHINESE JOURNAL OF INFECTIOUS DISEASES
    2008年 8期 463-466 ,共4页

    徐葛林%孟胜利%吴杰%肖奇友%周敦金%朱凤才%王萍%王定明%明平刚%严家新%张永振%董关木%唐建蓉%杨晓明

    서갈림%맹성리%오걸%초기우%주돈금%주봉재%왕평%왕정명%명평강%엄가신%장영진%동관목%당건용%양효명

    狂犬病%狂犬病病毒%酶联免疫吸附测定%荧光免疫测定%基因型%序列分析 狂犬病%狂犬病病毒%酶聯免疫吸附測定%熒光免疫測定%基因型%序列分析
    광견병%광견병병독%매련면역흡부측정%형광면역측정%기인형%서렬분석
    Rabies%Rabies virus%Enzyme-linked immunosorbent assay%Fluoroimmunoassay%Genotype%Sequence analysis
    目的 检测我国不同地区动物中狂犬病毒带毒率并分析糖蛋白编码基因序列.方法 El.ISA、免疫荧光法分别检测586份采集自中国不同地区的犬、猫,蝙蝠和野鼠脑标本和16份犬唾液中狂犬病街毒,阳性标本乳鼠颅内接种.并测序.结果 ELISA、免疫荧光法均在犬脑中分离到10株狂犬病街毒,其中贵州省113份犬脑中分离到2株病毒,湖南省62份犬脑中分离到2株病毒,武汉市70份犬脑中分离到2株病毒,江苏省85份犬脑中分离到4株病毒,沈阳市69份犬脑中未分离到狂犬病毒;79份猫脑、100份蝙蝠脑及8份鼠脑中未检出狂犬病街毒,16份犬唾液标本未检出狂犬病毒.在贵州省和武汉市,冬季采集的112份犬脑末检出狂犬病毒.春夏季采集的40份犬脑中,4份阳性.分离的10株狂犬病毒阳性株颅内接种乳鼠后.均发病死亡.所有分离株均属狂犬病毒基因1型,可分为4个亚组.结论 同一地区的狂犬病毒分离株以及相邻省份的狂犬病毒分离株的同源性十分接近,动物样本采集时间与狂犬病毒阳检率有关.
    목적 검측아국불동지구동물중광견병독대독솔병분석당단백편마기인서렬.방법 El.ISA、면역형광법분별검측586빈채집자중국불동지구적견、묘,편복화야서뇌표본화16빈견타액중광견병가독,양성표본유서로내접충.병측서.결과 ELISA、면역형광법균재견뇌중분리도10주광견병가독,기중귀주성113빈견뇌중분리도2주병독,호남성62빈견뇌중분리도2주병독,무한시70빈견뇌중분리도2주병독,강소성85빈견뇌중분리도4주병독,침양시69빈견뇌중미분리도광견병독;79빈묘뇌、100빈편복뇌급8빈서뇌중미검출광견병가독,16빈견타액표본미검출광견병독.재귀주성화무한시,동계채집적112빈견뇌말검출광견병독.춘하계채집적40빈견뇌중,4빈양성.분리적10주광견병독양성주로내접충유서후.균발병사망.소유분리주균속광견병독기인1형,가분위4개아조.결론 동일지구적광견병독분리주이급상린성빈적광견병독분리주적동원성십분접근,동물양본채집시간여광견병독양검솔유관.
    Objective To detect the positive rate of rabies virus in animals of different regions in China and to analyze the sequences of glycoprotein encoding gene.Methods Rabies street viruses were detected in 586 brain samples of dogs,cats,bats,mice and 16 saliva samples of dogs from different regions in China using enzyme-linked immunosorbent assay(ELISA)and immunofluorescent assay (IFA).Positive samples were inoculated intracranially to neonate rats and then sequenced.Results Ten street virus strains were isolated from dog brains using ELISA and IFA,in which 2 positive of 113 in Guizhou Province,2 positive of 62 in Hunan Province,2 positive of 70 in Wuban city,4 positive of 85 in Jiangsu Province,and no positive of 69 in Shenyang city.There was no detectable rabies virus in brains of 79 cats,100 bats,8 mice and in 16 saliva samples.One hundred and twelve brain samples from dogs collected in winter were all negative and 4 were positive Of 40 samples collected in spring and summer in Guizhou Province and Wuhan city.The neonate rats all died of rabies after inoculated with isolated street virus strains.All the 10 isolates were genotype 1 of Lyssavirus and could be divided into 4 SUbgroups.Conclusions The rabies virus isolates are highly homologous in the same region and the adjaeent provinces.The positive rate of rabies virus isolation in animals is related to the time of sample collection.
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