中华传染病杂志
中華傳染病雜誌
중화전염병잡지
CHINESE JOURNAL OF INFECTIOUS DISEASES
2008年
8期
467-470
,共4页
饶英%莫凌%钟敏%孟成艳%张西燕%虞胜镭%陈刚%王莹%王菲菲%陈嘉臻%朱静%张文宏
饒英%莫凌%鐘敏%孟成豔%張西燕%虞勝鐳%陳剛%王瑩%王菲菲%陳嘉臻%硃靜%張文宏
요영%막릉%종민%맹성염%장서연%우성뢰%진강%왕형%왕비비%진가진%주정%장문굉
结核,肺%酶联免疫斑点法%T淋巴细胞%抗原%细菌
結覈,肺%酶聯免疫斑點法%T淋巴細胞%抗原%細菌
결핵,폐%매련면역반점법%T림파세포%항원%세균
Tuberculosis,pulmonary%Enzyme-linked immunospot assay%Tolymphocytes%Antigen,bacterial
目的 评价我国酶联免疫斑点法(ELISPOT)用于诊断肺结核患者的应用价值.方法 结核特异性ELISPOT法检测76例诊断明确的肺结核患者外周血单核细胞.同时选择30例无结核接触史、胸部X线片无异常且PPI)皮试阴性的成年人作为对照.数据采用卡方检验和非参数Mann-Whitney检验.结果 76例患者总体阳性率为71.0%,健康对照组阳性率为6.7%.按照患者抗结核疗程分为治疗1个月内、治疗6个月内、治疗6个月以卜3组,阳性率分别为87.5%、74.1%和52.0%(X2=35.63,P=0.000).该技术在初治结核患者中的灵敏度为87.5%,特异度为93.3%,阳性预测值为91.3%,阴性预测值为90.3%.结论 结核特异性ELISPOT技术在我国初治(抗结核化疗疗程1个月内)的结核病患者中具有较高诊断价值.
目的 評價我國酶聯免疫斑點法(ELISPOT)用于診斷肺結覈患者的應用價值.方法 結覈特異性ELISPOT法檢測76例診斷明確的肺結覈患者外週血單覈細胞.同時選擇30例無結覈接觸史、胸部X線片無異常且PPI)皮試陰性的成年人作為對照.數據採用卡方檢驗和非參數Mann-Whitney檢驗.結果 76例患者總體暘性率為71.0%,健康對照組暘性率為6.7%.按照患者抗結覈療程分為治療1箇月內、治療6箇月內、治療6箇月以蔔3組,暘性率分彆為87.5%、74.1%和52.0%(X2=35.63,P=0.000).該技術在初治結覈患者中的靈敏度為87.5%,特異度為93.3%,暘性預測值為91.3%,陰性預測值為90.3%.結論 結覈特異性ELISPOT技術在我國初治(抗結覈化療療程1箇月內)的結覈病患者中具有較高診斷價值.
목적 평개아국매련면역반점법(ELISPOT)용우진단폐결핵환자적응용개치.방법 결핵특이성ELISPOT법검측76례진단명학적폐결핵환자외주혈단핵세포.동시선택30례무결핵접촉사、흉부X선편무이상차PPI)피시음성적성년인작위대조.수거채용잡방검험화비삼수Mann-Whitney검험.결과 76례환자총체양성솔위71.0%,건강대조조양성솔위6.7%.안조환자항결핵료정분위치료1개월내、치료6개월내、치료6개월이복3조,양성솔분별위87.5%、74.1%화52.0%(X2=35.63,P=0.000).해기술재초치결핵환자중적령민도위87.5%,특이도위93.3%,양성예측치위91.3%,음성예측치위90.3%.결론 결핵특이성ELISPOT기술재아국초치(항결핵화료료정1개월내)적결핵병환자중구유교고진단개치.
Objective To evaluate the application of a new enzyme-linked immunospot assay (ELSIPOT)based on Mycobacterium tuberculosis-sepeific T cells detection in the diagnosis of active pulmonary tuberculosis in China.Methods Using the ELlSPOT assay.T cells specific for early secreted antigenic target 6-kDa protein(ESAT-6)and culture filtrate protein 10(CFP-10)in the peripheral blood from 76 active pulmonary tuberculosis patients were measured.Thirty healthy people were recruited as healthy controls,which had no tuberculosis contacting history and were normal for chest X ray examination as well as negative for tuberculosis skin test(TST).The data were analyzed using Chi-square test and Mann-Whitney test.Results The positive rate of ELISPOT was 71.0%(54/76)and 6.7%(2/30)in the patients with active pulmonarytuberculosis and healthy controls respectively.Furthermore,the positive rate was as high as 87.5% in patients with antituberculosis treatment course less than 1 month,and decreased to 74.1% and 52.0% in the patients with the treatment course varying from 1 to 6 months and more than 6 months respectively(X2=35.63,P=0.000).In the patients with treatment course less than 1 month,the sensitivity,specificity,positive predictive value and negative predictive value of ELISPOT were 87.5 oA,93.3 oA,91.3% and 90.3%,respectively.Conclusion ELISPOT assay is a sensitive assay for detecting tuberculosis infection,especially in the patients with anti-tuberculosis treatment course less than 1 month.
