CAJ | 학술논문

目的分析近5年上海地区甲型流行性感冒(流感)病毒亚型的分布并探究其原因.方法对上海地区流感监测哨点流感样患者和聚集性流感暴发患者,采集咽拭子标本接种犬肾细胞(MDCK),直接荧光免疫法鉴定阳性分离株型别,多重RT-PCR鉴定亚型.结果 2004年至2005年初的季节性流感以A/H3N2为主;2005年末至2006年中期的季节性流感以A/H1N1为主;2006年末到2007年10月份分离到的流感毒株基本为A/H3N2亚型;2008年1月至5月份,人群流感病毒分离株仍以A/H3N2处于优势地位,但从2008年7月开始的季节性流感则以A/H1N1占绝对优势.结论近5年上海地区季节性流感主要为甲型流感病毒H3N2和H1N1亚型,但在不同年份的季节性流感中流行强度有所不同.
목적 분석근5년상해지구갑형류행성감모(류감)병독아형적분포병탐구기원인.방법 대상해지구류감감측초점류감양환자화취집성류감폭발환자,채집인식자표본접충견신세포(MDCK),직접형광면역법감정양성분리주형별,다중RT-PCR감정아형.결과 2004년지2005년초적계절성류감이A/H3N2위주;2005년말지2006년중기적계절성류감이A/H1N1위주;2006년말도2007년10월빈분리도적류감독주기본위A/H3N2아형;2008년1월지5월빈,인군류감병독분리주잉이A/H3N2처우우세지위,단종2008년7월개시적계절성류감칙이A/H1N1점절대우세.결론 근5년상해지구계절성류감주요위갑형류감병독H3N2화H1N1아형,단재불동년빈적계절성류감중류행강도유소불동.
Objective To analysis the distribution of subtype of influenza A of 2004-2008 in Shanghai area and to explore the reason.Methods Throat swabs were collected from patients with influenza-like illness in the sentinel surveillance clinic in Shanghai from 2004 to 2008,and inoculated in Madin-Darby canine kidney(MDCK)cells.Influenza viruses were identified and typed by direct immunofluorescence assay(DIF)and sub-typed by multiplex reverse transcriptase-polymerase chain reaction (rnRT-PCR).Results During seasonal epidemic of influenza from 2004 to 2008,influenza A/H3N2 viruses dominated from 2004 to the beginning of 2005,and then were subrogated by A/H1N1 from the end of 2005 to middle 2006.Subsequently,influenza A/H3N2 again contributed to most influenza events from the end of 2006 to Oct.2007 and kept predominating from Jan to May in 2008 while A/H1N1 became dominating since Jul.2008.Conclusion Though A/H3N2 and A/H1N1 were main seasonal influenza A subtypes in recent 5 years in Shanghai,the dominated subtype varied in different season.