中华传染病杂志
中華傳染病雜誌
중화전염병잡지
CHINESE JOURNAL OF INFECTIOUS DISEASES
2012年
11期
663-667
,共5页
郭健%高怡%郭珍%付振东%郝海燕%汪波%王素萍
郭健%高怡%郭珍%付振東%郝海燕%汪波%王素萍
곽건%고이%곽진%부진동%학해연%왕파%왕소평
肝炎病毒,乙型%乙型肝炎e抗原%DNA,病毒%婴儿,新生%树突细胞%传染病传播,垂直
肝炎病毒,乙型%乙型肝炎e抗原%DNA,病毒%嬰兒,新生%樹突細胞%傳染病傳播,垂直
간염병독,을형%을형간염e항원%DNA,병독%영인,신생%수돌세포%전염병전파,수직
Hepatitis B virus%Hepatitis B e antigens%DNA,viral%Infant,newborn%Dendritic cells%Infectious disease transmission,vertical
目的 探讨HBsAg阳性母亲新生儿树突状细胞(DC)数量变化的影响因素.方法 收集2011年7月至2012年3月在太原市第三人民医院妇产科分娩的HBsAg阳性孕妇及其新生儿60对.流式细胞术测定新生儿DC亚群数量,化学发光免疫试验检测母亲及新生儿出生24 h内、主被动免疫前的外周血HBV血清学标志物,荧光定量PCR检测HBV DNA.两组样本比较采用两独立样本秩和检验中的Mann-Whitney检验和t检验,相关分析采用Spearman秩相关分析和卡方检验.结果 60名新生儿中,5名为HBsAg阳性、HBV DNA阴性；60例HBsAg阳性孕妇中,HBeAg阳性21例,HBV DNA阳性29例.HBV宫内感染组新生儿外周血髓样DC (mDC)、浆细胞样DC(pDC)与非感染组比较,差异无统计学意义(Z=-0.535,Z=-0.027;均P＞0.05).母亲HBeAg阳性与新生儿HBeAg阳性密切相关(Pearson列联系数为0.928,P＜0.01).HBeAg阳性和HBeAg阴性母亲所生新生儿外周血mDC分别为0.60±0.57和0.87±0.58(Z=-2.085,P＜0.05).HBV DNA阳性母亲所生新生儿外周血mDC、pDC与HBV DNA阴性者比较,差异无统计学意义(均P＞0.05)；母亲外周血HBV DNA＞1×107拷贝/mL与≤1×103拷贝/mL比较时,其新生儿外周血pDC明显下降(0.30±0.18比0.64±0.55,t=-2.996,P=0.005).结论 母亲HBeAg阳性可能使新生儿mDC数量下降,而HBV DNA＞1×10 7拷贝/mL时,可能会使新生儿pDC数量减少.
目的 探討HBsAg暘性母親新生兒樹突狀細胞(DC)數量變化的影響因素.方法 收集2011年7月至2012年3月在太原市第三人民醫院婦產科分娩的HBsAg暘性孕婦及其新生兒60對.流式細胞術測定新生兒DC亞群數量,化學髮光免疫試驗檢測母親及新生兒齣生24 h內、主被動免疫前的外週血HBV血清學標誌物,熒光定量PCR檢測HBV DNA.兩組樣本比較採用兩獨立樣本秩和檢驗中的Mann-Whitney檢驗和t檢驗,相關分析採用Spearman秩相關分析和卡方檢驗.結果 60名新生兒中,5名為HBsAg暘性、HBV DNA陰性；60例HBsAg暘性孕婦中,HBeAg暘性21例,HBV DNA暘性29例.HBV宮內感染組新生兒外週血髓樣DC (mDC)、漿細胞樣DC(pDC)與非感染組比較,差異無統計學意義(Z=-0.535,Z=-0.027;均P＞0.05).母親HBeAg暘性與新生兒HBeAg暘性密切相關(Pearson列聯繫數為0.928,P＜0.01).HBeAg暘性和HBeAg陰性母親所生新生兒外週血mDC分彆為0.60±0.57和0.87±0.58(Z=-2.085,P＜0.05).HBV DNA暘性母親所生新生兒外週血mDC、pDC與HBV DNA陰性者比較,差異無統計學意義(均P＞0.05)；母親外週血HBV DNA＞1×107拷貝/mL與≤1×103拷貝/mL比較時,其新生兒外週血pDC明顯下降(0.30±0.18比0.64±0.55,t=-2.996,P=0.005).結論 母親HBeAg暘性可能使新生兒mDC數量下降,而HBV DNA＞1×10 7拷貝/mL時,可能會使新生兒pDC數量減少.
목적 탐토HBsAg양성모친신생인수돌상세포(DC)수량변화적영향인소.방법 수집2011년7월지2012년3월재태원시제삼인민의원부산과분면적HBsAg양성잉부급기신생인60대.류식세포술측정신생인DC아군수량,화학발광면역시험검측모친급신생인출생24 h내、주피동면역전적외주혈HBV혈청학표지물,형광정량PCR검측HBV DNA.량조양본비교채용량독립양본질화검험중적Mann-Whitney검험화t검험,상관분석채용Spearman질상관분석화잡방검험.결과 60명신생인중,5명위HBsAg양성、HBV DNA음성；60례HBsAg양성잉부중,HBeAg양성21례,HBV DNA양성29례.HBV궁내감염조신생인외주혈수양DC (mDC)、장세포양DC(pDC)여비감염조비교,차이무통계학의의(Z=-0.535,Z=-0.027;균P＞0.05).모친HBeAg양성여신생인HBeAg양성밀절상관(Pearson렬련계수위0.928,P＜0.01).HBeAg양성화HBeAg음성모친소생신생인외주혈mDC분별위0.60±0.57화0.87±0.58(Z=-2.085,P＜0.05).HBV DNA양성모친소생신생인외주혈mDC、pDC여HBV DNA음성자비교,차이무통계학의의(균P＞0.05)；모친외주혈HBV DNA＞1×107고패/mL여≤1×103고패/mL비교시,기신생인외주혈pDC명현하강(0.30±0.18비0.64±0.55,t=-2.996,P=0.005).결론 모친HBeAg양성가능사신생인mDC수량하강,이HBV DNA＞1×10 7고패/mL시,가능회사신생인pDC수량감소.
Objective To investigate the influence factors of quantitative changes of dendritic cells (DC) in neonate born to HBsAg positive mother.Methods Sixty HBsAg positive mothers and their newborns were enrolled from the Third People's Hospital of Taiyuan from July 2011 to March 2012.The serum hepatitis B virus (HBV) markers and HBV DNA in mothers and newborns before vaccination were determined by chemiluminescence immunoassay (CLIA) and fluorescence quantitative polymerase chain reaction (PCR).The circulating frequencies of DC subsets were determined in the newborns by flow cytometry (FCM).The comparison of data was done by Mann-Whitney test and t test.The correlation analysis was done by Spearman rank correlation analysis and chi square test.Results Among 60 newborns,5 were HBsAg positive and HBV DNA negative.Among 60 HBsAg positive mothers,21 were HBeAg positive and 29 were HBV DNA positive.There was no significant quantitative difference of neonatal myeloid dendritic cells (mDC) and plasmacytoid dendritic cells (pDC) between intrauterine infection group and intrauterine non-infection group (Z=-0.535,P=0.59 and Z=-0.027,P=0.98,respectively).However,mother's HBeAg positive status was closely related with neonatal HBeAg positive status (Pearson contingency coefficient was 0.928,P＜0.01).The frequencies of mDC in newborns born to HBeAg positive mothers were significantly lower than those born to HBeAg negative mothers (0.60±0.57 vs 0.87±0.58; Z=-2.085,P＜0.05).However,there was no significant quantitative differences of mDC and pDC between newborns born to HBV DNA positive mothers and born to negative mothers (Z=-1.272,P=0.20 and Z=-0.806,P=0.42,respectively).The frequencies of pDC were significantly lower in newborns born to mothers with HBV DNA＞ 1 × 107 copy/mL compared to newborns born to HBV DNA negative mothers (0.30±0.18 vs 0.64±0.55; t=-2.996,P=0.005).Conclusions HBeAg positive status of mothers may reduce neonatal frequencies of mDC.Neonatal frequencies of pDC may be reduced when the mothers' HBV DNA loads are more than 1 × 107 copy/mL.
