中华传染病杂志
中華傳染病雜誌
중화전염병잡지
CHINESE JOURNAL OF INFECTIOUS DISEASES
2012年
11期
668-672
,共5页
魏俊妮%张玥%薛淑莲%张军峰%双杰钰%冯丽萍%王素萍
魏俊妮%張玥%薛淑蓮%張軍峰%雙傑鈺%馮麗萍%王素萍
위준니%장모%설숙련%장군봉%쌍걸옥%풍려평%왕소평
胎盘%肝炎表面抗原,乙型%免疫组织化学%肝炎病毒,乙型%婴儿,新生
胎盤%肝炎錶麵抗原,乙型%免疫組織化學%肝炎病毒,乙型%嬰兒,新生
태반%간염표면항원,을형%면역조직화학%간염병독,을형%영인,신생
Placenta%Hepatitis B surface antigens%Immunohistochemistry%Hepatitis B virus%Infant,newborn
目的 探讨HBsAg阳性孕妇胎盘HBsAg与新生儿HBV血清学标志物和HBVDNA的关系.方法 免疫组织化学亲和素-生物素复合物(ABC)法检测155例HBsAg阳性孕妇的胎盘HBsAg,ELISA法检测新生儿HBV血清学标志物,荧光定量PCR法检测新生儿血清HBVDNA.各指标阳性率的比较采用x2检验.结果 155例孕妇的胎盘各型细胞均存在不同程度的HBsAg阳性表达,总的胎盘HBsAg阳性58份,阳性率为37.4％；蜕膜细胞、滋养层细胞、绒毛间质细胞和绒毛毛细血管内皮细胞HBsAg阳性各有58、40、29和11份,阳性率分别为37.4％、25.8％、18.7％和7.1％；胎盘从母体面的蜕膜细胞至胎儿面的绒毛毛细血管内皮细胞HBsAg阳性率逐渐下降(趋势x2=43.01,P=0.00).胎盘HBsAg阳性与新生儿HBsAg、HBeAg阳性均有关(x2=4.88,P＜0.05;x2 =3.86,P＜0.05),而与新生儿抗-HBe、抗-HBc无关(x2=3.36,P＞0.05;x2=0.00,P＞0.05).胎儿面的绒毛毛细血管内皮细胞和绒毛间质细胞HBsAg阳性时,新生儿HBsAg阳性的危险性高(OR=5.31,95 ％CI:1.38～20.40；OR=3.33,95％CI:1.16～9.52)；而滋养层细胞和绒毛间质细胞HBsAg阳性时,新生儿HBeAg阳性的危险性较大(OR=3.04,95％ CI:1.45～6.39;OR=3.05,95％CI:1.32～7.03)；胎盘HBsAg阳性与新生儿HBV DNA阳性无关(x2=0.09,P＞0.05).结论 越接近胎儿面的胎盘细胞HBsAg表达阳性,新生儿HBV血清学标志物阳性的危险性越大.HBsAg在胎盘中以“逐层转移”的方式进入胎儿血循环.
目的 探討HBsAg暘性孕婦胎盤HBsAg與新生兒HBV血清學標誌物和HBVDNA的關繫.方法 免疫組織化學親和素-生物素複閤物(ABC)法檢測155例HBsAg暘性孕婦的胎盤HBsAg,ELISA法檢測新生兒HBV血清學標誌物,熒光定量PCR法檢測新生兒血清HBVDNA.各指標暘性率的比較採用x2檢驗.結果 155例孕婦的胎盤各型細胞均存在不同程度的HBsAg暘性錶達,總的胎盤HBsAg暘性58份,暘性率為37.4％；蛻膜細胞、滋養層細胞、絨毛間質細胞和絨毛毛細血管內皮細胞HBsAg暘性各有58、40、29和11份,暘性率分彆為37.4％、25.8％、18.7％和7.1％；胎盤從母體麵的蛻膜細胞至胎兒麵的絨毛毛細血管內皮細胞HBsAg暘性率逐漸下降(趨勢x2=43.01,P=0.00).胎盤HBsAg暘性與新生兒HBsAg、HBeAg暘性均有關(x2=4.88,P＜0.05;x2 =3.86,P＜0.05),而與新生兒抗-HBe、抗-HBc無關(x2=3.36,P＞0.05;x2=0.00,P＞0.05).胎兒麵的絨毛毛細血管內皮細胞和絨毛間質細胞HBsAg暘性時,新生兒HBsAg暘性的危險性高(OR=5.31,95 ％CI:1.38～20.40；OR=3.33,95％CI:1.16～9.52)；而滋養層細胞和絨毛間質細胞HBsAg暘性時,新生兒HBeAg暘性的危險性較大(OR=3.04,95％ CI:1.45～6.39;OR=3.05,95％CI:1.32～7.03)；胎盤HBsAg暘性與新生兒HBV DNA暘性無關(x2=0.09,P＞0.05).結論 越接近胎兒麵的胎盤細胞HBsAg錶達暘性,新生兒HBV血清學標誌物暘性的危險性越大.HBsAg在胎盤中以“逐層轉移”的方式進入胎兒血循環.
목적 탐토HBsAg양성잉부태반HBsAg여신생인HBV혈청학표지물화HBVDNA적관계.방법 면역조직화학친화소-생물소복합물(ABC)법검측155례HBsAg양성잉부적태반HBsAg,ELISA법검측신생인HBV혈청학표지물,형광정량PCR법검측신생인혈청HBVDNA.각지표양성솔적비교채용x2검험.결과 155례잉부적태반각형세포균존재불동정도적HBsAg양성표체,총적태반HBsAg양성58빈,양성솔위37.4％；세막세포、자양층세포、융모간질세포화융모모세혈관내피세포HBsAg양성각유58、40、29화11빈,양성솔분별위37.4％、25.8％、18.7％화7.1％；태반종모체면적세막세포지태인면적융모모세혈관내피세포HBsAg양성솔축점하강(추세x2=43.01,P=0.00).태반HBsAg양성여신생인HBsAg、HBeAg양성균유관(x2=4.88,P＜0.05;x2 =3.86,P＜0.05),이여신생인항-HBe、항-HBc무관(x2=3.36,P＞0.05;x2=0.00,P＞0.05).태인면적융모모세혈관내피세포화융모간질세포HBsAg양성시,신생인HBsAg양성적위험성고(OR=5.31,95 ％CI:1.38～20.40；OR=3.33,95％CI:1.16～9.52)；이자양층세포화융모간질세포HBsAg양성시,신생인HBeAg양성적위험성교대(OR=3.04,95％ CI:1.45～6.39;OR=3.05,95％CI:1.32～7.03)；태반HBsAg양성여신생인HBV DNA양성무관(x2=0.09,P＞0.05).결론 월접근태인면적태반세포HBsAg표체양성,신생인HBV혈청학표지물양성적위험성월대.HBsAg재태반중이“축층전이”적방식진입태인혈순배.
Objective To study the relationship between placenta HBsAg in HBsAg positive pregnant women and serum hepatitis B virus (HBV) markers,HBV DNA levels in newborns.Methods Placenta HBsAg was detected by immunohistochemical affinity hormone-biotin complex (ABC) method in 155 HBsAg positive pregnant women.Serum HBV markers in newborns were detected by enzyme-linked immunosorbent assay (ELISA).Serum HBV DNA levels of newborns were detected by real-time fluorescence quantitative polymerase chain reaction (PCR).The positive rates were compared using x2 test.Results HBsAg was expressed with different levels in various types of cells of placenta in 155 pregnant women.The total placenta HBsAg positive rate was 37.4％ (58/155),and those in decidual cells,trophoblastic cells,villous mesenchymal cells and villous capillary endothelial cells were 37.4％ (58/155),25.8％ (40/155),18.7％ (29/155) and 7.1％ (11/155),respectively.The HBsAg positive rates of placenta gradually decreased from decidual cells of the maternal surface to villous capillary endothelial cells of the fetal surface (tendency x2 =43.01,P=0.00).The positivity of placenta HBsAg was associated with both HBsAg and HBeAg in newborns (x2 =4.88,P＜0.05 and x2 =3.86,P＜0.05,respectively),while that was not associated withanti-HBe and anti-HBc in newborns (x2 =3.36,P＞0.05 and x2 =0.00,P＞ 0.05,respectively).The risk of HBsAg positive in newborns was higher when HBsAg was positive in villous capillary endothelial cells and villous mesenchymal cells (OR=5.31,95 ％CI=1.38-20.40 and OR=3.33,95％CI=1.16-9.52,respectively).The risk of HBeAg positive in newborns was higher when HBsAg was positive in trophoblastic cells and villous mesenchymal cells (OR=3.04,95 ％CI=1.45-6.39 and OR=3.05,95 ％ CI=1.32-7.03,respectively).However,placenta HBsAg positive was not associated with HBV DNA positive in newborns (x2 =0.09,P＞0.05).Conclusion The risk of neonatal HBV serological markers positive is higher when the HBsAg positive placental cells are closer to fetal surface,which indicates that HBsAg enters fetal blood circulation by means of cell transferring layer by layer.
