CAJ | 학술논문

目的比较犬肾细胞系(MDCK)、人喉表皮癌细胞(Hep-2)和非洲绿猴肾细胞(Vero)制备的MHV混合细胞与相应单一敏感细胞株对流行性感冒(流感)病毒、肠道病毒的分离结果.方法流感样患者咽拭子标本接种MHV混合细胞和MDCK细胞,手足口病患儿咽拭子或粪标本接种MHV混合细胞和Vero细胞,观察病毒致细胞病变效应(CPE),采用多重反转录-聚合酶链反应(mRT-PCR)检测甲、乙型流感病毒和肠道病毒.结果 MDCK和Vero细胞的CPE较MHV混合细胞明显.138份流感病毒接种MHV混合细胞,分离出34株流感病毒,分离率为24.6％；MDCK细胞分离出流感病毒39株,分离率为28.3％.MHV混合细胞与MDCK细胞流感病毒分离率比较,差异无统计学意义(x2=1.92,P＞0.05).32株肠道病毒接种MHV混合细胞,分离出肠道病毒9株,分离率为28.1％；Vero细胞分离出12株肠道病毒,分离率为37.5％.MHV混合细胞与Vero细胞肠道病毒分离率比较,差异无统计学意义(x2 =3.00,P＞0.05).结论 MHV混合细胞CPE不如单一细胞易于观察；MHV混合细胞适用于生物性突发公共卫生事件中,可分离培养临床标本中甲型、乙型流感病毒和表现为呼吸道症状的肠道病毒.
목적 비교견신세포계(MDCK)、인후표피암세포(Hep-2)화비주록후신세포(Vero)제비적MHV혼합세포여상응단일민감세포주대류행성감모(류감)병독、장도병독적분리결과.방법 류감양환자인식자표본접충MHV혼합세포화MDCK세포,수족구병환인인식자혹분표본접충MHV혼합세포화Vero세포,관찰병독치세포병변효응(CPE),채용다중반전록-취합매련반응(mRT-PCR)검측갑、을형류감병독화장도병독.결과 MDCK화Vero세포적CPE교MHV혼합세포명현.138빈류감병독접충MHV혼합세포,분리출34주류감병독,분리솔위24.6％；MDCK세포분리출류감병독39주,분리솔위28.3％.MHV혼합세포여MDCK세포류감병독분리솔비교,차이무통계학의의(x2=1.92,P＞0.05).32주장도병독접충MHV혼합세포,분리출장도병독9주,분리솔위28.1％；Vero세포분리출12주장도병독,분리솔위37.5％.MHV혼합세포여Vero세포장도병독분리솔비교,차이무통계학의의(x2 =3.00,P＞0.05).결론 MHV혼합세포CPE불여단일세포역우관찰；MHV혼합세포괄용우생물성돌발공공위생사건중,가분리배양림상표본중갑형、을형류감병독화표현위호흡도증상적장도병독.
Objective To evaluate shell vials of MHV,a combination of Madin-Darby canine kidney cells (MDCK),human epidermoid cancer cells (Hep-2) and African green monkey kidney cells (Vero),and conventional cell culture in detecting influenza viruses and enterovirus from fresh clinical specimens.Methods Specimens from patients with influenza-like illness and children with hand-foot-mouth disease were inoculated with both shell vials of MHV and MDCK/Vero.Then cytopathological effect (CPE) was examined daily.Influenza viruses and enteroviruses were detected by multiple reverse transcriptase-polymerase chain reaction (mRT-PCR).Results CPE of MDCK/Vero cells were stronger than the shell vials of MHV.The isolation rate of influenza virus by MHV was 24.6％ (34/138) and that by MDCK was 28.3％ (39/138),which was not significantly different (x2 =1.92,P＞0.05).That of enterovirus by MHV was 28.1％ (9/32) and that by Vero was 37.5％ (12/32),which was not significantly different (x2 =3.00.P＞0.05).Conclusions CPE in MDCK/Vero cells are easier to be observed than the shell vials of MHV.However,the shell vials of MHV are appropriate in public health emergencies,which can be used for isolation of influenza viruses and enterovirus in patients with respiratory symptoms.