中华传染病杂志
中華傳染病雜誌
중화전염병잡지
CHINESE JOURNAL OF INFECTIOUS DISEASES
2013年
1期
33-36
,共4页
脑膜炎,隐球菌性%纤维蛋白%单核细胞%中性白细胞
腦膜炎,隱毬菌性%纖維蛋白%單覈細胞%中性白細胞
뇌막염,은구균성%섬유단백%단핵세포%중성백세포
Meningitis,cryptococcal%Fibrin%Monocytes%Neutrophils
目的 探讨人纤维介素(hFgl2)的表达与隐球菌性脑膜炎(CM)的关系.方法 采用免疫组织化学法检测20例CM患者(CM组)和20名健康者(健康对照组)血WBC及11例配对CM患者脑脊液WBC hFgl2的表达情况,并用医学影像分析系统(MIAS)对CM患者血和脑脊液hFgl2的表达进行灰度分析(图像的平均灰度值与hFgl2蛋白表达呈负相关).结果 CM组和健康对照组血单核细胞中hFgl2蛋白表达的平均灰度值分别为114.49±11.82、137.56±10.64,中性粒细胞中hFgl2蛋白表达的平均灰度值分别为111.71±8.62、143.56±12.57；与健康对照者比较,CM患者血单核细胞和中性粒细胞hFgl2的表达均明显升高(t值分别为21.21和27.19,均P＜0.01).CM患者和阴性对照脑脊液单核细胞中hFgl2蛋白表达的平均灰度值分别为113.33±8.07、147.25±9.72,中性粒细胞中hFgl2蛋白表达的平均灰度值分别为115.44±8.67、149.25±8.57；与阴性对照比较,CM患者脑脊液单核细胞和中性粒细胞hFgl2的表达均明显升高(t值分别为29.95和28.87,均P＜0.01).CM患者的血与配对脑脊液的单核细胞和中性粒细胞中hFgl2蛋白表达的差异均无统计学意义(t值分别为0.924和0.607,均P＞0.05).结论 hFgl2可表达于CM患者的血和脑脊液单核细胞和中性粒细胞.
目的 探討人纖維介素(hFgl2)的錶達與隱毬菌性腦膜炎(CM)的關繫.方法 採用免疫組織化學法檢測20例CM患者(CM組)和20名健康者(健康對照組)血WBC及11例配對CM患者腦脊液WBC hFgl2的錶達情況,併用醫學影像分析繫統(MIAS)對CM患者血和腦脊液hFgl2的錶達進行灰度分析(圖像的平均灰度值與hFgl2蛋白錶達呈負相關).結果 CM組和健康對照組血單覈細胞中hFgl2蛋白錶達的平均灰度值分彆為114.49±11.82、137.56±10.64,中性粒細胞中hFgl2蛋白錶達的平均灰度值分彆為111.71±8.62、143.56±12.57；與健康對照者比較,CM患者血單覈細胞和中性粒細胞hFgl2的錶達均明顯升高(t值分彆為21.21和27.19,均P＜0.01).CM患者和陰性對照腦脊液單覈細胞中hFgl2蛋白錶達的平均灰度值分彆為113.33±8.07、147.25±9.72,中性粒細胞中hFgl2蛋白錶達的平均灰度值分彆為115.44±8.67、149.25±8.57；與陰性對照比較,CM患者腦脊液單覈細胞和中性粒細胞hFgl2的錶達均明顯升高(t值分彆為29.95和28.87,均P＜0.01).CM患者的血與配對腦脊液的單覈細胞和中性粒細胞中hFgl2蛋白錶達的差異均無統計學意義(t值分彆為0.924和0.607,均P＞0.05).結論 hFgl2可錶達于CM患者的血和腦脊液單覈細胞和中性粒細胞.
목적 탐토인섬유개소(hFgl2)적표체여은구균성뇌막염(CM)적관계.방법 채용면역조직화학법검측20례CM환자(CM조)화20명건강자(건강대조조)혈WBC급11례배대CM환자뇌척액WBC hFgl2적표체정황,병용의학영상분석계통(MIAS)대CM환자혈화뇌척액hFgl2적표체진행회도분석(도상적평균회도치여hFgl2단백표체정부상관).결과 CM조화건강대조조혈단핵세포중hFgl2단백표체적평균회도치분별위114.49±11.82、137.56±10.64,중성립세포중hFgl2단백표체적평균회도치분별위111.71±8.62、143.56±12.57；여건강대조자비교,CM환자혈단핵세포화중성립세포hFgl2적표체균명현승고(t치분별위21.21화27.19,균P＜0.01).CM환자화음성대조뇌척액단핵세포중hFgl2단백표체적평균회도치분별위113.33±8.07、147.25±9.72,중성립세포중hFgl2단백표체적평균회도치분별위115.44±8.67、149.25±8.57；여음성대조비교,CM환자뇌척액단핵세포화중성립세포hFgl2적표체균명현승고(t치분별위29.95화28.87,균P＜0.01).CM환자적혈여배대뇌척액적단핵세포화중성립세포중hFgl2단백표체적차이균무통계학의의(t치분별위0.924화0.607,균P＞0.05).결론 hFgl2가표체우CM환자적혈화뇌척액단핵세포화중성립세포.
Objective The purpose of this study was to explore the expression of human fibrinogen-like protein 2 (hFgl2) in patients with cryptococcal meningitis (CM).Methods The expression of hFgl2 in the peripheral blood leukocytes from 20 CM cases and 20 healthy individuals,and the expression of hFgl2 in cerebrospinal fluid (CSF) leukocytes in 11 pairs of CM patients and matched controls was detected using immunohistochemical methods.Medical image analysis system (MIAS) was used to analyze the expression of hFgl2 gray values,which were negatively correlated to the expression of hFgl2 protein in both peripheral blood and CSF.Results The average gray values for hFgl2 protein in peripheral monocytes of CM patients and healthy controls were 114.49± 11.82 and 137.56± 10.64,respectively,and those in peripheral neutrophils were 111.71 ± 8.62 and 143.56±12.57,respectively.Compared with the healthy control group,expressions of hFgl2 in peripheral blood monocytes and neutrophils were both significantly increased in CM patients (t =21.21,t=27.19; P＜0.01).The average gray values for hFgl2 protein in CSF monocytes from CM patients and negative controls were 115.44±8.67 and 149.25±8.57,respectively,and those in CSFneutrophils were 111.71±8.62 and 143.56 ± 12.57,respectively.The expressions of hFgl2 in CSFmonocytes and neutrophils were significantly increased in CM patients (t =29.95,t=28.87; P＜0.01),while the expression intensity of hFgl2 was similar when peripheral blood monocytes and neutrophils compared with their CSF counterparts (t=0.924,t=0.607; both P＞0.05).Conclusions hFgl2 can be expressed in both peripheral and CSF monocytes and neutrophils in CM patients.
