CAJ | 학술논문

目的探索HCV自发清除者、慢性丙型肝炎(丙肝)及健康人血清代谢组学的差异.方法纳入HCV自发清除者、慢性丙肝患者和健康对照各30例,采用快速液相色谱-串联质谱联用(LC-MS/MS)技术,应用主成分分析(PCA)、正交偏最小二乘法-判别分析(OPLS-DA)进行模式识别,然后通过变量重要性因子(VIP)、非参数检验,结合数据库检索筛选鉴定有差异的代谢物.结果 25个变量被确认为存在显著差异的代谢物,其中7个变量被鉴定为花生四烯酸、棕榈油酸、葵酰基肉碱、溶血磷脂酰胆碱(20∶5,16∶0)、溶血磷脂酰乙醇胺(16∶0,18∶0),涉及脂肪酸、磷脂等代谢.其中花生四烯酸以及未鉴定出明确结构的m/z 179.0719、m/z 382.1360、m/z 548.3475、m/z680.4281、m/z 303.2323等物质与自发清除组的相关性较好,受试者工作特征曲线下面积为0.887～0.977,具有较好的特异度和敏感度.结论 HCV自发清除者、慢性丙肝感染者、健康人在血清代谢水平上存在明显差异,这些差异的意义有待进一步探索.
목적 탐색HCV자발청제자、만성병형간염(병간)급건강인혈청대사조학적차이.방법 납입HCV자발청제자、만성병간환자화건강대조각30례,채용쾌속액상색보-천련질보련용(LC-MS/MS)기술,응용주성분분석(PCA)、정교편최소이승법-판별분석(OPLS-DA)진행모식식별,연후통과변량중요성인자(VIP)、비삼수검험,결합수거고검색사선감정유차이적대사물.결과 25개변량피학인위존재현저차이적대사물,기중7개변량피감정위화생사희산、종려유산、규선기육감、용혈린지선담감(20∶5,16∶0)、용혈린지선을순알(16∶0,18∶0),섭급지방산、린지등대사.기중화생사희산이급미감정출명학결구적m/z 179.0719、m/z 382.1360、m/z 548.3475、m/z680.4281、m/z 303.2323등물질여자발청제조적상관성교호,수시자공작특정곡선하면적위0.887～0.977,구유교호적특이도화민감도.결론 HCV자발청제자、만성병간감염자、건강인재혈청대사수평상존재명현차이,저사차이적의의유대진일보탐색.
Objective To explore the difference of serum metabonomics among patients with spontaneous clearance of hepatitis C virus (HCV),patients with chronic hepatitis C (CHC) and healthy controls.Methods Thirty patients with spontaneous clearance of HCV,30 patients with CHC and 30 healthy controls were enrolled in this study.Rapid liquid chromatography-tandem mass spectrometry (LC-MS/MS) and performed pattern recognition by principal component analysis (PCA)and orthogonal partial least squares discriminant analysis (OPLS-DA) in each group was applied.Then several metabolites which were different among the 3 groups through variable importance in projection (VIP) value,non-parametric test and screening in databases was identified.Results Twenty-five variables that were significantly different among the 3 groups were considered as potential biomarkers,among which 7 variables were identified as arachidonic acid,palmitoleic acid,dodecanoylcarnitine,lysophosphatidylcholine { LysoPC [20∶5 (5Z,8Z,11Z,14Z,17Z)]},LysoPC (16∶0),Lysophosphatidylethanolamine [LysoPE (18∶0)],LysoPE (16∶0),all related to the metabolism of fatty acid and phospholipids.Especially,there was a good correlation between arachidonic acid as well as some other variables whose structures were not identified clearly,such as m/z 179.0719,m/z 382.1360,m/z 548.3475,m/z 680.4281 and m/z 303.2323,and patients with spontaneous clearance of HCV,with an area under the curve (AUC) from 0.887 to 0.977 and good specificity and sensitivity.Conclusion A marked difference of serum metabolic profiles exists among patients with spontaneous clearance of HCV,patients with CHC and healthy controls,and its clinical significance requires further study.