CAJ | 학술논문

目的通过对苏州市2004年至2010年麻疹发病情况及流行株的基因型别和特征进行分析,了解麻疹发病情况及流行株基因特点,为加强麻疹病毒学监测,实现消除麻疹提供科学依据.方法收集2004年至2010年苏州市麻疹病例,分析麻疹的流行病学特征,采集2009年至2011年57例麻疹患者咽拭子和尿液标本分离病毒,提取病毒RNA,通过RT-PCR扩增麻疹病毒N基因C末端456 bp核苷酸并进行序列测定,与GenBank提供的麻疹各基因型参考株进行比较.结果 2004年至2010年苏州市麻疹发病率为12.85/10万,其中发病率最高为2005年,之后麻疹发病率开始下降.麻疹发病年龄主要集中在1岁以内及20～30岁两个年龄段,发病人群以散居儿童为主.从57例麻疹病例中分离到4株麻疹病毒株,其中3株为H1a型,与世界卫生组织(WHO)推荐的H1型基因代表株(China9 3-2、China93-7、China94-7)比较,同源性为98.46％～99.12％；而另1株则为A型,与A型代表株Shanghai191的同源性＞99％.结论 2005年后苏州市麻疹发病率呈下降趋势,麻疹发病年龄主要集中在1岁以内及20～30岁两个年龄段.2009年至2011年苏州地区麻疹流行株主要由H1a基因型引起,但也分离到A基因型.
목적 통과대소주시2004년지2010년마진발병정황급류행주적기인형별화특정진행분석,료해마진발병정황급류행주기인특점,위가강마진병독학감측,실현소제마진제공과학의거.방법 수집2004년지2010년소주시마진병례,분석마진적류행병학특정,채집2009년지2011년57례마진환자인식자화뇨액표본분리병독,제취병독RNA,통과RT-PCR확증마진병독N기인C말단456 bp핵감산병진행서렬측정,여GenBank제공적마진각기인형삼고주진행비교.결과 2004년지2010년소주시마진발병솔위12.85/10만,기중발병솔최고위2005년,지후마진발병솔개시하강.마진발병년령주요집중재1세이내급20～30세량개년령단,발병인군이산거인동위주.종57례마진병례중분리도4주마진병독주,기중3주위H1a형,여세계위생조직(WHO)추천적H1형기인대표주(China9 3-2、China93-7、China94-7)비교,동원성위98.46％～99.12％；이령1주칙위A형,여A형대표주Shanghai191적동원성＞99％.결론 2005년후소주시마진발병솔정하강추세,마진발병년령주요집중재1세이내급20～30세량개년령단.2009년지2011년소주지구마진류행주주요유H1a기인형인기,단야분리도A기인형.
Objective To understand the incidence of measles and the genetic characteristics of epidemic strains in Suzhou city from 2004 to 2010,and to provide scientific basis for strengthened measles surveillance and elimination of measles.Methods Disease data of measles cases in Suzhou city during 2004 to 2010 were collected for epidemiological analysis.RNAs were extracted from measles virus isolated from throat swab specimens collected from suspected measles cases during 2009 to 2011.The 456 bp fragment of C terminus of nucleoprotein (N) gene was amplified by reverse transcriptasepolymerase chain reaction.Sequence analysis was conducted to ascertain the genotype and compare the difference of nucleotide with other measles virus strain registered in GenBank.Results The annual average incidence of measles was 12.85/100 000 during 2004 to 2010.Incidence of measles peaked in 2005 and then declined.Children younger than 1 year and adults 20-30 years were dominantly infected.Migrant children were main cases of all cases.Four measles viruses were isolated from 57 throat swab specimens,and sequence analysis indicated that 3 of them belonged to H1a genotype.The 456 nucleotides in the terminal of the N gene shared 98.46 ％-99.12％ homogeneity with the World Health Organization (WHO) recommend H1 genotype reference strains (China93-2,China93-7,and China94-7).Another one of the 4 strains belonged to A genotype,with 99％ genetic homogeneity compared with Shanghai191.Conclusions The incidence of measles declines after the year 2005.The majority of isolates are H1a genotype,while a genotype A strain is also isolated.