CAJ | 학술논문

目的通过监测辽宁省哨点医院病毒性腹泻病例,了解病毒性腹泻病原流行特征,探讨病毒性腹泻病原变异和流行规律.方法收集2009年1月至2011年12月辽宁省哨点医院粪便标本639份,采用PCR和RT-PCR检测轮状病毒、杯状病毒、星状病毒和腺病毒.计数资料分析采用x2检验或Fisher确切概率法.结果 639份粪便标本中轮状病毒阳性率为15.96％,杯状病毒为11.25％,星状病毒为1.25％,腺病毒为0.31％.检出A组轮状病毒101份,G3、P[8]型为优势株；C组轮状病毒1份,为辽宁省首次检出,并通过分子生物学分析,C组轮状病毒核苷酸序列JX407109与日本暴发疫情核苷酸序列AB648916亲缘关系较近,核苷酸序列一致性达到99％.检出杯状病毒72份,包括诺如病毒70份,为GⅡ/4型,札如病毒2份.检出星状病毒8份,以1型为主.检出腺病毒2份为41型.辽宁省病毒性腹泻高发月份为12月至次年2月,轮状病毒和星状病毒以5岁以下儿童高发,杯状病毒感染无年龄差异.结论辽宁省病毒性腹泻主要病原为A组轮状病毒和杯状病毒,C组轮状病毒与日本暴发疫情核苷酸序列较近,需引起注意.
목적 통과감측요녕성초점의원병독성복사병례,료해병독성복사병원류행특정,탐토병독성복사병원변이화류행규률.방법 수집2009년1월지2011년12월요녕성초점의원분편표본639빈,채용PCR화RT-PCR검측륜상병독、배상병독、성상병독화선병독.계수자료분석채용x2검험혹Fisher학절개솔법.결과 639빈분편표본중륜상병독양성솔위15.96％,배상병독위11.25％,성상병독위1.25％,선병독위0.31％.검출A조륜상병독101빈,G3、P[8]형위우세주；C조륜상병독1빈,위요녕성수차검출,병통과분자생물학분석,C조륜상병독핵감산서렬JX407109여일본폭발역정핵감산서렬AB648916친연관계교근,핵감산서렬일치성체도99％.검출배상병독72빈,포괄낙여병독70빈,위GⅡ/4형,찰여병독2빈.검출성상병독8빈,이1형위주.검출선병독2빈위41형.요녕성병독성복사고발월빈위12월지차년2월,륜상병독화성상병독이5세이하인동고발,배상병독감염무년령차이.결론 요녕성병독성복사주요병원위A조륜상병독화배상병독,C조륜상병독여일본폭발역정핵감산서렬교근,수인기주의.
Objective To understand the epidemiologic characteristics and pathogenic virus of cases of viral diarrhea in sentinel hospitals in Liaoning Province.Methods From Jan 2009 to Dec 2011,639 stool samples from sentinel hospitals of Liaoning Province were collected.Rotavirus,human calicivirus,astrovirus and adenovirus were detected by polymerase chain reaction and reverse transcriptase-polymerase chain reaction.The data analysis used chi-squanetest and Fisher's exact test.Results Rotavirus,human calicivirus,astrovirus and adenovirus were detected in 15.96 ％,11.25 ％,1.25％ and 0.31％ of the 639 specimens,respectively.G3 was the most prevailing serotype and P[8] was the most common genotype among 101 group A rotavirus isolates.One strain of group C rotavirus was also detected,which was reported for the first time from Liaoning Province.Phylogenetic analysis showed that this group C rotavirus JX407109 in the present study had the closest genetic relationship with the outbreak strain AB648916 from Japan,with nucleotide sequence consistency of 99 ％.Among the 72 samples of human calicivirus,70 samples were norovirus with G Ⅱ/4 being the predominant genotype,and 2 samples were sapovirus.Astrovirus was detected in 8 samples,most of which were genotype 1.Adenovirus was detected in 2 samples,and both were genotype 41.High incidences of viral diarrhea were noted during the months from December to next year February,and children under 5 years of age had high incidence of rotavirus and astrovirus,while the incidence of calicivirus were similar among different age groups.Conclusions The predominant pathogens of viral diarrhea in Liaoning Province are group A rotavirus and calicivirus.Notably,the group C rotavirus in Liaoning Province shares high genetic consistency with the outbreak strain from Japan.