中华传染病杂志
中華傳染病雜誌
중화전염병잡지
CHINESE JOURNAL OF INFECTIOUS DISEASES
2013年
4期
216-220
,共5页
唐漾波%占晗琳%曹孟丽%赵稳%平岖
唐漾波%佔晗琳%曹孟麗%趙穩%平嶇
당양파%점함림%조맹려%조은%평구
HIV-1%肝炎病毒属%重叠感染%CD4阳性T淋巴细胞%CD8阳性T淋巴细胞%酶联免疫斑点测定
HIV-1%肝炎病毒屬%重疊感染%CD4暘性T淋巴細胞%CD8暘性T淋巴細胞%酶聯免疫斑點測定
HIV-1%간염병독속%중첩감염%CD4양성T림파세포%CD8양성T림파세포%매련면역반점측정
HIV-1%Hepacivirus%Superinfection%CD4-positive T-lymphocytes%CD8-positive T-lymphocytes%Enzyme-linked immunospot assay
目的 了解单纯感染HIV-1及合并感染HCV时HIV-1特异性T淋巴细胞免疫应答的特点.方法 单纯HIV-1感染者26例和HIV-1/HCV合并感染者23例,采用免疫磁珠分选技术从外周血单个核细胞(PBMC)中分选出CD4+T淋巴细胞及CD8+T淋巴细胞,采用酶联免疫斑点测定(ELISPOT),以HIV-1 P24编码区的氨基酸序列人工合成12个重叠肽段组成的肽段库作为特异性抗原表位,测定CD4-T淋巴细胞、CD8+T淋巴细胞及PBMC的IFN-γ分泌细胞频数；实时荧光定量PCR检测HIV-1 RNA.组间比较采用单因素方差分析及Mann-WhitneyU检验,Spearman 评价组间相关性.结果 单纯HIV-1感染者的HIV-1抗原特异性CD4+T淋巴细胞中位数为25斑点形成细胞(SFC)/106细胞,显著低于CD8+T淋巴细胞的38 SFC/106细胞(F=4.592,P-0.037)及PBMC的53 SFC/106细胞(F=5.436,P=0.025).HIV-1/HCV合并感染者的HIV-1抗原特异性CD4+T淋巴细胞(中位数为5 SFC/106细胞,Z=-2.432,P=0.015)、CD8+T淋巴细胞(中位数为5 SFC/106细胞,Z=-1.996,P—0.046)及PBMC(中位数为10 SFC/106细胞,Z=-2.306,P-0.021)的应答频数显著低于单纯HIV-1感染者.结论 在HIV-1感染中,CD4+T淋巴细胞能对HIV-1产生一定的特异性免疫应答,但是其强度弱于CD8+T淋巴细胞；合并HCV感染可能使HIV-1感染者的HIV-1特异性T淋巴细胞应答强度下调.
目的 瞭解單純感染HIV-1及閤併感染HCV時HIV-1特異性T淋巴細胞免疫應答的特點.方法 單純HIV-1感染者26例和HIV-1/HCV閤併感染者23例,採用免疫磁珠分選技術從外週血單箇覈細胞(PBMC)中分選齣CD4+T淋巴細胞及CD8+T淋巴細胞,採用酶聯免疫斑點測定(ELISPOT),以HIV-1 P24編碼區的氨基痠序列人工閤成12箇重疊肽段組成的肽段庫作為特異性抗原錶位,測定CD4-T淋巴細胞、CD8+T淋巴細胞及PBMC的IFN-γ分泌細胞頻數；實時熒光定量PCR檢測HIV-1 RNA.組間比較採用單因素方差分析及Mann-WhitneyU檢驗,Spearman 評價組間相關性.結果 單純HIV-1感染者的HIV-1抗原特異性CD4+T淋巴細胞中位數為25斑點形成細胞(SFC)/106細胞,顯著低于CD8+T淋巴細胞的38 SFC/106細胞(F=4.592,P-0.037)及PBMC的53 SFC/106細胞(F=5.436,P=0.025).HIV-1/HCV閤併感染者的HIV-1抗原特異性CD4+T淋巴細胞(中位數為5 SFC/106細胞,Z=-2.432,P=0.015)、CD8+T淋巴細胞(中位數為5 SFC/106細胞,Z=-1.996,P—0.046)及PBMC(中位數為10 SFC/106細胞,Z=-2.306,P-0.021)的應答頻數顯著低于單純HIV-1感染者.結論 在HIV-1感染中,CD4+T淋巴細胞能對HIV-1產生一定的特異性免疫應答,但是其彊度弱于CD8+T淋巴細胞；閤併HCV感染可能使HIV-1感染者的HIV-1特異性T淋巴細胞應答彊度下調.
목적 료해단순감염HIV-1급합병감염HCV시HIV-1특이성T림파세포면역응답적특점.방법 단순HIV-1감염자26례화HIV-1/HCV합병감염자23례,채용면역자주분선기술종외주혈단개핵세포(PBMC)중분선출CD4+T림파세포급CD8+T림파세포,채용매련면역반점측정(ELISPOT),이HIV-1 P24편마구적안기산서렬인공합성12개중첩태단조성적태단고작위특이성항원표위,측정CD4-T림파세포、CD8+T림파세포급PBMC적IFN-γ분비세포빈수；실시형광정량PCR검측HIV-1 RNA.조간비교채용단인소방차분석급Mann-WhitneyU검험,Spearman 평개조간상관성.결과 단순HIV-1감염자적HIV-1항원특이성CD4+T림파세포중위수위25반점형성세포(SFC)/106세포,현저저우CD8+T림파세포적38 SFC/106세포(F=4.592,P-0.037)급PBMC적53 SFC/106세포(F=5.436,P=0.025).HIV-1/HCV합병감염자적HIV-1항원특이성CD4+T림파세포(중위수위5 SFC/106세포,Z=-2.432,P=0.015)、CD8+T림파세포(중위수위5 SFC/106세포,Z=-1.996,P—0.046)급PBMC(중위수위10 SFC/106세포,Z=-2.306,P-0.021)적응답빈수현저저우단순HIV-1감염자.결론 재HIV-1감염중,CD4+T림파세포능대HIV-1산생일정적특이성면역응답,단시기강도약우CD8+T림파세포；합병HCV감염가능사HIV-1감염자적HIV-1특이성T림파세포응답강도하조.
Objective To investigate the features of immune response of human immunodeficiency virus type-1 (HIV-1) antigen specific T lymphocytes in HIV-1 monoinfected or HIV 1/hepatitis C virus (HCV) coinfected individuals.Methods Twenty-six HIV-1 monoinfected and 23 HIV-1/HCV coinfected individuals were enrolled.Immunomagnetic microbeads were used to isolate T lymphocyte subpopulation CD4+ T cells and CD8+ T cells from human peripheral blood mononuclear cells (PBMC).Frequencies of interferon-γ (IFN-γ) secreting cells of CD4+,CD8+ T lymphocytes and PBMC stimulated by a peptide pool containing 12 overlapping peptides in HIV-1 P24 from 49 patients were assessed by enzyme-linked immunospot (ELISPOT) assay.HIV-1 RNA levels of these patients were also detected by real-time fluorescence quantitative polymerase chain reaction.The data were compared by one-way ANOVA and Mann-Whitney U test,and Spearman test was used for correlation analysis.Results Frequencies of HIV-1 antigen specific CD4+ T lymphocytes [median =25 spot-forming cells (SFC)/106 cells] were significantly lower than those of CD8+T lymphocytes (median=38SFC/106 cells,F=4.592,P=0.037) and PBMC (median=53 SFC/106 cells,F=5.436,P=0.025) in HIV-1 monoinfected group.Frequencies of HIV-1 antigen specific CD4+ T lymphocytes (median=5 SFC/106 cells,Z=-2.432,P=0.015),CD8+T lymphocytes (median=5 SFC/106 cells,Z=-1.996,P=0.046) and PBMC (median=10 SFC/106 cells,Z=-2.306,P=0.021) in HIV-1/HCV coinfected group were significantly lower than those in HIV-1 monoinfected group.Conclusions In HIV-1 infection,antigen specific immune response of CD4+ T cells can be activated,but weaker than that of CD8+ T cells.Co-infection with HCV might down-regulate the responses of HIV-1 antigen specific T lymphocytes in HIV-1 infected individuals.
