中华传染病杂志
中華傳染病雜誌
중화전염병잡지
CHINESE JOURNAL OF INFECTIOUS DISEASES
2013年
5期
269-273
,共5页
刘真真%陈永平%沈宇娟%王雅琴%宋梅%林镯
劉真真%陳永平%瀋宇娟%王雅琴%宋梅%林鐲
류진진%진영평%침우연%왕아금%송매%림탁
髓磷脂蛋白质类%转化生子因子β1%肝硬化%DNA结合蛋白质类%疾病模型,动物%动力传导,细胞
髓燐脂蛋白質類%轉化生子因子β1%肝硬化%DNA結閤蛋白質類%疾病模型,動物%動力傳導,細胞
수린지단백질류%전화생자인자β1%간경화%DNA결합단백질류%질병모형,동물%동력전도,세포
Myelin proteins%Transforming growth factor beta1%Liver cirrhosis%DNA-binding proteins%Disease models,animal%Mechanotransduction,cellular
目的 探讨在小鼠肝纤维化模型中Nogo-B与转化生长因子-β1(TGF-β1)/Smad2信号通路的关系.方法 将24只健康雄性ICR小鼠分为正常对照组6只和肝纤维化模型组18只,模型组再按造模后不同时间点分为4、8和12周3个亚组.肝纤维化模型组皮下注射四氯化碳(CCl4)诱导小鼠肝纤维化模型,肝组织经HE和Masson染色后,光学显微镜下观察各组肝组织病理变化；RT-PCR方法检测各组肝脏Nogo-B、Smad2和TGF-β1 mRNA表达；Western印迹法、免疫组织化学法检测各组肝脏Nogo-B、Smad2、TGF-β1蛋白表达.组间均数比较采用单因素方差分析.结果 CCl4成功诱导肝纤维化模型.Nogo-B两种亚型Nogo-B1、Nogo-B2 mRNA在正常肝组织表达为0.140±0.050和0.104±0.023,在肝纤维化模型组肝脏中表达显著增加,造模12周时为1.054±0.040和0.500±0.057(F=431.41、135.46,均P＜0.01)；Nogo-B蛋白主要表达于肝脏间质中,肝细胞仅少量表达；且Nogo-B mRNA及蛋白的表达强度与参与肝纤维化信号通路相关因子Smad2、TGF-β1 mRNA及蛋白均呈正相关(均P＜0.01).结论 Nogo-B可能通过参与TGF-β1/Smad2信号通路在肝纤维化发展过程中起重要作用,促进肝纤维化的发展.
目的 探討在小鼠肝纖維化模型中Nogo-B與轉化生長因子-β1(TGF-β1)/Smad2信號通路的關繫.方法 將24隻健康雄性ICR小鼠分為正常對照組6隻和肝纖維化模型組18隻,模型組再按造模後不同時間點分為4、8和12週3箇亞組.肝纖維化模型組皮下註射四氯化碳(CCl4)誘導小鼠肝纖維化模型,肝組織經HE和Masson染色後,光學顯微鏡下觀察各組肝組織病理變化；RT-PCR方法檢測各組肝髒Nogo-B、Smad2和TGF-β1 mRNA錶達；Western印跡法、免疫組織化學法檢測各組肝髒Nogo-B、Smad2、TGF-β1蛋白錶達.組間均數比較採用單因素方差分析.結果 CCl4成功誘導肝纖維化模型.Nogo-B兩種亞型Nogo-B1、Nogo-B2 mRNA在正常肝組織錶達為0.140±0.050和0.104±0.023,在肝纖維化模型組肝髒中錶達顯著增加,造模12週時為1.054±0.040和0.500±0.057(F=431.41、135.46,均P＜0.01)；Nogo-B蛋白主要錶達于肝髒間質中,肝細胞僅少量錶達；且Nogo-B mRNA及蛋白的錶達彊度與參與肝纖維化信號通路相關因子Smad2、TGF-β1 mRNA及蛋白均呈正相關(均P＜0.01).結論 Nogo-B可能通過參與TGF-β1/Smad2信號通路在肝纖維化髮展過程中起重要作用,促進肝纖維化的髮展.
목적 탐토재소서간섬유화모형중Nogo-B여전화생장인자-β1(TGF-β1)/Smad2신호통로적관계.방법 장24지건강웅성ICR소서분위정상대조조6지화간섬유화모형조18지,모형조재안조모후불동시간점분위4、8화12주3개아조.간섬유화모형조피하주사사록화탄(CCl4)유도소서간섬유화모형,간조직경HE화Masson염색후,광학현미경하관찰각조간조직병리변화；RT-PCR방법검측각조간장Nogo-B、Smad2화TGF-β1 mRNA표체；Western인적법、면역조직화학법검측각조간장Nogo-B、Smad2、TGF-β1단백표체.조간균수비교채용단인소방차분석.결과 CCl4성공유도간섬유화모형.Nogo-B량충아형Nogo-B1、Nogo-B2 mRNA재정상간조직표체위0.140±0.050화0.104±0.023,재간섬유화모형조간장중표체현저증가,조모12주시위1.054±0.040화0.500±0.057(F=431.41、135.46,균P＜0.01)；Nogo-B단백주요표체우간장간질중,간세포부소량표체；차Nogo-B mRNA급단백적표체강도여삼여간섬유화신호통로상관인자Smad2、TGF-β1 mRNA급단백균정정상관(균P＜0.01).결론 Nogo-B가능통과삼여TGF-β1/Smad2신호통로재간섬유화발전과정중기중요작용,촉진간섬유화적발전.
Objective To study the relationship between Nogo-B and transforming growth factor-β1 (TGF-β1)/Smad2 signaling pathway in mice models of hepatic fibrosis.Methods Twenty four healthy male ICR mice were divided into two groups,with 6 in the control group and 18 in the model group.Mice in the model group were further divided into three subgroups according to different time points:subgroups of 4,8 and 12 weeks,with 6 mice in each subgroup.Hepatic fibrosis of mice was induced by subcutaneous injection of carbon tetrachloride (CCl4).The histopathologic changes of the liver were observed by optical microscope using hematoxylin-eosin and Masson trichrome stainings of the liver tissues.Expressions of Nogo-B,Smad2 and TGF-β1 mRNA and proteins in liver were detected by reverse transcription-polymerase chain reaction (RT-PCR),Western blot and immunohistochemistry assays,respectively.Means among groups were compared by univariate analysis of variance.Results The hepatic fibrosis models were successfully induced by CCl4 injection.The expressions of two subtypes of Nogo-B,Nogo-B1 and Nogo-B2 mRNA in normal livers were 0.140±0.050 and 0.104±0.023,but both significantly increased in the livers of mice in the 12 week model subgroup (1.054±0.040 and 0.500±0.057,F=431.41 and 135.46,respectively; both P＜0.01).The Nogo-B protein was mainly expressed in nonparenchymal cells of the liver,and was hardly expressed in hepatocytes.Linear correlation analysis showed that the expressions of Nogo-B mRNA and proteins were positively correlated with Smad2 and TGF-β1 mRNA and proteins (all P＜0.01),which were considered to participate in the signaling pathway of hepatic fibrosis.Conclusion Nogo-B might play a role in the development and progression of hepatic fibrosis by participating in TGF-β1/Smad2 signaling pathway.
