CAJ | 학술논문

目的分析整合子类型、耐药基因盒在志贺菌中的分布及其与耐药性之间的关系.方法采用最低抑菌浓度(M IC)测定志贺菌药物敏感性,PCR扩增Ⅰ、Ⅱ和Ⅲ类整合子及其耐药基因盒,限制性内切酶及DNA测序确定各基因序列.结果在分离的62株志贺菌中,57株存在多重耐药现象,占91.9％.在多重耐药株中,Ⅰ类、Ⅱ类整合子基因阳性株分别为52和55株,分别占91.2％和96.5％(其中仅含Ⅰ类整合子2株,仅含Ⅱ类5株,Ⅰ类、Ⅱ类整合子均含的50株).未检测到Ⅲ类整合子基因.典型Ⅰ类整合子8株,耐药基因盒为dfrV和dfrA 17 aadA5;非典型Ⅰ类整合子44株,耐药基因盒均为blaOXA0-aadA1.6株福氏志贺菌中存在典型和非典型Ⅰ类整合子共存现象.Ⅱ类整合子耐药基因盒均为dfrA1sat1-aadA1.结论本研究分离的志贺菌存在严重多重耐药,多重耐药株中普遍携带非典型Ⅰ类整合子和Ⅱ类整合子的耐药基因盒,部分菌株存在典型和非典型Ⅰ类整合子共存现象.
목적 분석정합자류형、내약기인합재지하균중적분포급기여내약성지간적관계.방법 채용최저억균농도(M IC)측정지하균약물민감성,PCR확증Ⅰ、Ⅱ화Ⅲ류정합자급기내약기인합,한제성내절매급DNA측서학정각기인서렬.결과 재분리적62주지하균중,57주존재다중내약현상,점91.9％.재다중내약주중,Ⅰ류、Ⅱ류정합자기인양성주분별위52화55주,분별점91.2％화96.5％(기중부함Ⅰ류정합자2주,부함Ⅱ류5주,Ⅰ류、Ⅱ류정합자균함적50주).미검측도Ⅲ류정합자기인.전형Ⅰ류정합자8주,내약기인합위dfrV화dfrA 17 aadA5;비전형Ⅰ류정합자44주,내약기인합균위blaOXA0-aadA1.6주복씨지하균중존재전형화비전형Ⅰ류정합자공존현상.Ⅱ류정합자내약기인합균위dfrA1sat1-aadA1.결론 본연구분리적지하균존재엄중다중내약,다중내약주중보편휴대비전형Ⅰ류정합자화Ⅱ류정합자적내약기인합,부분균주존재전형화비전형Ⅰ류정합자공존현상.
Objective To analyze the distribution of class 1,2 and 3 integrons and their gene cassettes,and to explore its relationship with drug resistance in Shigella.Methods Antimicrobial susceptibility was detected by minimal inhibitory concentration (MIC) method.All the genes of integrons and gene cassettes were amplified by polymerase chain reaction (PCR).The amplicons were identified by amplified fragment length polymorphism (AFLP) and sequencing.Results Fifty seven multi-drug resistant strains were identified from a total of 62 Shigella strains (91.9％).Among multi drug resistant strains,52 strains carried integrons of class 1 (91.2 ％) and 55 strains carried integrons of class 2 (96.5％).Only 2 strains carried class 1 integrons alone,5 strains carried class 2 integrons alone and 50 strains had both class 1 and class 2 integrons.Class 3 integrons were not detected.The gene cassettes of typical class 1 integrons,dfrV and dfrA17-aadA5,were detected in 6 strains and 2 strains,respectively.Atypical class 1 integrons with gene cassettes blaOxA30 aadA1 were detected in 44 strains.The typical and atypical class 1 integrons coexisted in 6 Shigella flexneri strains.Gene cassettes for class 2 integrons were dfrA1 sat1-aadA1.Conclusions The multi-drug resistant Shigella strains are widely distributed in Ji'nan,and the atypical class 1 integrons and class 2 integrons are common in these strains.Coexistence of the two integrons is observed in some of the strains.