CAJ | 학술논문

目的了解1株多耐药肺炎克雷伯菌JM45株对氨基糖苷类药物耐药的遗传学背景.方法采用罗氏454高通量测序技术对肺炎克雷伯菌JM45株做全基因组测序(完成图),再分析氨基糖苷类修饰酶基因、16S核糖体RNA(rRNA)甲基化酶基因检测状况,并和其他已经完成全基因组测序的革兰阴性菌作比较基因组学分析.结果最终得到肺炎克雷伯菌JM45株1条完整的基因组(染色体)序列及2条质粒序列.基因组(染色体)序列为5 273 812 bp(GC含量65.8％),质粒1序列为317 156 bp(GC含量53.0％),质粒2序列为12 209 bp(GC含量55.3％).在质粒1中发现了氨基糖苷类腺苷转移酶基因aadA2(KPN_5186)和16S rRNA甲基化酶基因rmtB(KPN_5243).由全基因组测序可见aadA2基因位于整合子中,排序为int ⅠA-dfrA12 aadA2 orf3/qacE;rmtB基因位于转座子中,排序为tnpA-TEM1 rmtB.结论携带aadA2和rmtB基因是JM45株耐氨基糖苷类药物的主要原因.
목적 료해1주다내약폐염극뢰백균JM45주대안기당감류약물내약적유전학배경.방법 채용라씨454고통량측서기술대폐염극뢰백균JM45주주전기인조측서(완성도),재분석안기당감류수식매기인、16S핵당체RNA(rRNA)갑기화매기인검측상황,병화기타이경완성전기인조측서적혁란음성균작비교기인조학분석.결과 최종득도폐염극뢰백균JM45주1조완정적기인조(염색체)서렬급2조질립서렬.기인조(염색체)서렬위5 273 812 bp(GC함량65.8％),질립1서렬위317 156 bp(GC함량53.0％),질립2서렬위12 209 bp(GC함량55.3％).재질립1중발현료안기당감류선감전이매기인aadA2(KPN_5186)화16S rRNA갑기화매기인rmtB(KPN_5243).유전기인조측서가견aadA2기인위우정합자중,배서위int ⅠA-dfrA12 aadA2 orf3/qacE;rmtB기인위우전좌자중,배서위tnpA-TEM1 rmtB.결론 휴대aadA2화rmtB기인시JM45주내안기당감류약물적주요원인.
Objective To investigate genetic background of aminoglycoside resistance in multi drug resistant Klebsiella pneumoniae JM45 isolate.Methods Whole genome sequencing (complete graph) was performed by high throughput Roche 454 sequencing approach.Aminoglycoside modifying enzyme genes and 16S rRNA methylase genes were further analyzed,and comparative genomics analysis was performed with other Gram-negative bacteria.Results A complete genome (chromosome) sequence and two plasmid sequences were obtained in JM45.The size of chromosome was5 273 812 bp (GC content:65.8％),the size of plasmid 1 was 317 156 bp (GC content:53.0％),and that of plasmid 2 was 12 209 bp (GC content:55.3％).Aminoglycoside adenosine transferase gene (aadA2,Feature ID:KPN_5186) and 16S rRNA methylase genes (rmtB,Feature ID:KPN_5243) were positive in plasmid 1.According to whole genome sequencing,aadA2 located in the integron and the order was intⅠA-dfrA12 aadA2 orf3/qacE; rmtB located in the transposon and the order was tnpATEM-1-rmtB.Conclusion aadA2 and rmtB play a key role in aminoglycoside resistance in JM45.