中华传染病杂志
中華傳染病雜誌
중화전염병잡지
CHINESE JOURNAL OF INFECTIOUS DISEASES
2013年
6期
331-335
,共5页
叶景荣%苏雪丽%郝明强%辛若雷%卢红艳%白立石%余双庆%冯霞%闫红
葉景榮%囌雪麗%郝明彊%辛若雷%盧紅豔%白立石%餘雙慶%馮霞%閆紅
협경영%소설려%학명강%신약뢰%로홍염%백립석%여쌍경%풍하%염홍
HIV-1%逆转录聚合酶链反应%流行病学,分子%北京
HIV-1%逆轉錄聚閤酶鏈反應%流行病學,分子%北京
HIV-1%역전록취합매련반응%류행병학,분자%북경
HIV-1%Reverse transcriptase polymerase chain reaction%Epidemiology,molecular%Beijing
目的 分析北京市HIV 1 B亚型的分子流行特征.方法 采集北京市2006年至2010年新发现的HIV 1 B亚型感染者抗凝血,反转录-套式PCR扩增病毒gag基因,并进行系统进化和Entropy核苷酸多态性差异分析.结果 成功扩增167份标本,其中欧美B亚型10份,B'亚型54份,中国B亚型101份.B'亚型进化簇内存在多条来自泰国和印度的序列,中国B亚型独立成簇,进化簇内无其他国家的序列.中国B亚型存在4个亚簇,71.1％的病例为男男性传播.以欧美B进化簇为参照,B'亚型和中国B亚型进化簇分别存在55和9 4个有明显差异的核苷酸位点.结论 北京市存在3种类型的HIV-1 B亚型,其中1种为本土株.
目的 分析北京市HIV 1 B亞型的分子流行特徵.方法 採集北京市2006年至2010年新髮現的HIV 1 B亞型感染者抗凝血,反轉錄-套式PCR擴增病毒gag基因,併進行繫統進化和Entropy覈苷痠多態性差異分析.結果 成功擴增167份標本,其中歐美B亞型10份,B'亞型54份,中國B亞型101份.B'亞型進化簇內存在多條來自泰國和印度的序列,中國B亞型獨立成簇,進化簇內無其他國傢的序列.中國B亞型存在4箇亞簇,71.1％的病例為男男性傳播.以歐美B進化簇為參照,B'亞型和中國B亞型進化簇分彆存在55和9 4箇有明顯差異的覈苷痠位點.結論 北京市存在3種類型的HIV-1 B亞型,其中1種為本土株.
목적 분석북경시HIV 1 B아형적분자류행특정.방법 채집북경시2006년지2010년신발현적HIV 1 B아형감염자항응혈,반전록-투식PCR확증병독gag기인,병진행계통진화화Entropy핵감산다태성차이분석.결과 성공확증167빈표본,기중구미B아형10빈,B'아형54빈,중국B아형101빈.B'아형진화족내존재다조래자태국화인도적서렬,중국B아형독립성족,진화족내무기타국가적서렬.중국B아형존재4개아족,71.1％적병례위남남성전파.이구미B진화족위삼조,B'아형화중국B아형진화족분별존재55화9 4개유명현차이적핵감산위점.결론 북경시존재3충류형적HIV-1 B아형,기중1충위본토주.
Objective To analyze the molecular epidemiological characteristics of human immunodeficiency virus 1 (HIV-1) B subtype strains in Beijing.Methods Plasma samples were collected from 200 newly diagnosed HIV 1 B subtype individuals reported during 2006 to 2010 in Beijing.The gag gene fragments were amplified from RNA template extracted from plasma using reverse transcriptation and nested polymerase chain reaction (PCR) methods and the sequences were analyzed by phylogenetic and Entropy analysis.Results A total of 167 sequences were successfully amplified from the gag genes.Phylogenetic analysis indicated that the subtype B sequences in Beijing could be grouped into three clades,which were named Europe and America B clade (10 sequences),B' clade (54 sequences) and China B clade (101 sequences) based on the origin of the reference sequences.China clade was pure Chinese B subtype clade which did not contain any foreign strains and it was segregated into four major distinct clusters,of which 71.1 ％ individuals was men who have sex with men (MSM).When compared with Europe and America clade,there were 55 and 94 significantly different nucleotides polymorphism composition sites in B'clade and China clade,respectively.Conclusion Three main epidemic clades exist in HIV1 B strains in Beijing,including one local China B clade.
