中华传染病杂志
中華傳染病雜誌
중화전염병잡지
CHINESE JOURNAL OF INFECTIOUS DISEASES
2013年
10期
586-592
,共7页
邢同京%徐洪涛%余文庆%王变%张晶
邢同京%徐洪濤%餘文慶%王變%張晶
형동경%서홍도%여문경%왕변%장정
肝炎,乙型,慢性%微RNAs%免疫耐受%基因表达
肝炎,乙型,慢性%微RNAs%免疫耐受%基因錶達
간염,을형,만성%미RNAs%면역내수%기인표체
Hepatitis B,chronic%MicroRNAs%Immune tolerance%Gene expression
目的 探讨不同阶段慢性HBV感染者微小RNA(miRNA)的分子表达谱及其临床意义.方法 应用miRNA芯片筛选12例慢性乙型肝炎,12例慢性HBV携带者,12例非活动性HBsAg携带者和9名健康对照者的miRNA分子表达谱.实时荧光定量PCR方法验证21例慢性乙型肝炎,23例慢性HBV携带者,20例非活动性HBsAg携带者和21名健康对照者的miRNA分子表达谱.正态分布资料比较采用两样本均数t检验,多组间比较采用方差分析和SNK-q检验；偏态分布资料比较采用Mann-Whitney法、Kruskal Wallis H法分析；受试者工作特征曲线(ROC曲线)分析miRNA分子的诊断价值.结果 与健康对照组比较,慢性HBV携带者外周血单个核细胞中miRNA分子上调2个,下调18个；慢性乙型肝炎患者miRNA分子上调33个,下调19个；非活动性HBsAg携带者miRNA分子上调2个,下调3个.实时荧光定量PCR方法检测6个miRNA分子结果与芯片检测的表达趋势基本一致.hsa-miR-4711-3p、hsa-miR-3191-5p、hsa-miR-5704-5p、hsamiR-548ah-5p、hsa-miR-146a 5p和hsa miR-29b-3p区分慢性HBV携带者与慢性乙型肝炎的ROC曲线下面积分别为0.994、0.984、0.967、1.000、1.000和0.996.结论 应用差异表达的miRNA分子可以区分不同阶段的慢性HBV感染者,差异表达的miRNA与HBV感染的免疫耐受和清除有关.
目的 探討不同階段慢性HBV感染者微小RNA(miRNA)的分子錶達譜及其臨床意義.方法 應用miRNA芯片篩選12例慢性乙型肝炎,12例慢性HBV攜帶者,12例非活動性HBsAg攜帶者和9名健康對照者的miRNA分子錶達譜.實時熒光定量PCR方法驗證21例慢性乙型肝炎,23例慢性HBV攜帶者,20例非活動性HBsAg攜帶者和21名健康對照者的miRNA分子錶達譜.正態分佈資料比較採用兩樣本均數t檢驗,多組間比較採用方差分析和SNK-q檢驗；偏態分佈資料比較採用Mann-Whitney法、Kruskal Wallis H法分析；受試者工作特徵麯線(ROC麯線)分析miRNA分子的診斷價值.結果 與健康對照組比較,慢性HBV攜帶者外週血單箇覈細胞中miRNA分子上調2箇,下調18箇；慢性乙型肝炎患者miRNA分子上調33箇,下調19箇；非活動性HBsAg攜帶者miRNA分子上調2箇,下調3箇.實時熒光定量PCR方法檢測6箇miRNA分子結果與芯片檢測的錶達趨勢基本一緻.hsa-miR-4711-3p、hsa-miR-3191-5p、hsa-miR-5704-5p、hsamiR-548ah-5p、hsa-miR-146a 5p和hsa miR-29b-3p區分慢性HBV攜帶者與慢性乙型肝炎的ROC麯線下麵積分彆為0.994、0.984、0.967、1.000、1.000和0.996.結論 應用差異錶達的miRNA分子可以區分不同階段的慢性HBV感染者,差異錶達的miRNA與HBV感染的免疫耐受和清除有關.
목적 탐토불동계단만성HBV감염자미소RNA(miRNA)적분자표체보급기림상의의.방법 응용miRNA심편사선12례만성을형간염,12례만성HBV휴대자,12례비활동성HBsAg휴대자화9명건강대조자적miRNA분자표체보.실시형광정량PCR방법험증21례만성을형간염,23례만성HBV휴대자,20례비활동성HBsAg휴대자화21명건강대조자적miRNA분자표체보.정태분포자료비교채용량양본균수t검험,다조간비교채용방차분석화SNK-q검험；편태분포자료비교채용Mann-Whitney법、Kruskal Wallis H법분석；수시자공작특정곡선(ROC곡선)분석miRNA분자적진단개치.결과 여건강대조조비교,만성HBV휴대자외주혈단개핵세포중miRNA분자상조2개,하조18개；만성을형간염환자miRNA분자상조33개,하조19개；비활동성HBsAg휴대자miRNA분자상조2개,하조3개.실시형광정량PCR방법검측6개miRNA분자결과여심편검측적표체추세기본일치.hsa-miR-4711-3p、hsa-miR-3191-5p、hsa-miR-5704-5p、hsamiR-548ah-5p、hsa-miR-146a 5p화hsa miR-29b-3p구분만성HBV휴대자여만성을형간염적ROC곡선하면적분별위0.994、0.984、0.967、1.000、1.000화0.996.결론 응용차이표체적miRNA분자가이구분불동계단적만성HBV감염자,차이표체적miRNA여HBV감염적면역내수화청제유관.
Objective To explore the expression profiles and their clinical significance of microRNA (miRNA) molecules in different stages of chronic hepatitis B virus (HBV) infection.Methods The miRNA molecule expressions of 12 patients with chronic hepatitis B,12 chronic HBV carriers,12 inactive hepatitis B surface antigen (HBsAg) carriers,and 9 healthy controls were screened using miRNA microarray.The miRNA profiles were validated by the real time fluorescence quantitative polymerase chain reaction (PCR).The t-test was used for comparison between twogroups,whereas one-way ANOVA and SNK-q tests were used for multiple comparisons.Mann-Whitney and Kruskal Wallis H tests were used for comparison of two or more groups of data with skewed distribution.The receiver operation characteristic (ROC) curve was constructed to evaluate the diagnostic significance of miRNA molecules.Results Compared with the healthy controls,significant differences in the expression profiles of miRNA molecules were found in peripheral blood mononuclear cells of chronic HBV carriers (2 molecules up-regulated,and 18 down regulated) and chronic hepatitis B patients (33 molecules up-regulated and 19 down-regulated).No significant difference was found between inactive HBsAg carriers (2 molecules up regulated,and 3 down-regulated) and controls.The results of six miRNA molecules detected by real-time fluorescence quantitative PCR were basically consistent with the results detected by microarray.The area under ROC curve of the six miRNA molecules of hsa miR-4711-3p,hsa-miR-3191 5p,hsa-miR-5704-5p,hsa-miR 548ah-5p,hsa-miR-146a-5p and hsa-miR-29b-3p in distinguishing immune tolerance and clearance of chronic HBV infection were 0.994,0.984,0.967,1.000,1.000 and 0.996,respectively.Conclusions The different expression profiles of miRNA molecules could be used to distinguish the different stages of chronic HBV infection,and are closely related with the immune tolerance and activation in chronic HBV infection.
